Recombinant Mouse P-Selectin/CD62P Fc Chimera Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse P-Selectin/CD62P Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. P-Selectin/Fc Chimera immobilized at 10 µg/mL will induce greater than 50% adhesion on U937 cells.
Optimal dilutions should be determined be each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse P-Selectin/CD62P protein
Mouse P-Selectin
(Trp42 - Ala709)
Accession # Q01102
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Trp42
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Selp
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
99.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
190 ‑ 200 kDa, reducing conditions
Publications
Read Publications using
737-PS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse P-Selectin/CD62P Fc Chimera Protein, CF

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

  1. Kansas, G.S. (1996) Blood 88:3259.
  2. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.

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Publications for P-Selectin/CD62P (737-PS)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Adhesion Assay, Binding Assay, Bioassay, Flow, In Vivo.


Filter By Application
Adhesion Assay
(1)
Binding Assay
(1)
Bioassay
(13)
Flow
(4)
In Vivo
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(17)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using 737-PS Applications Species
FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus Elife, 2018;7(0):. 2018 [PMID: 30187863]
Y Pan, T Tian, CO Park, SY Lofftus, S Mei, X Liu, C Luo, JT O'Malley, A Gehad, JE Teague, SJ Divito, R Fuhlbrigge, P Puigserver, JG Krueger, GS Hotamislig, RA Clark, TS Kupper Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism Nature, 2017;0(0):. 2017 [PMID: 28219080] (Bioassay, Human) Bioassay Human
X Jiang, CO Park, J Geddes Swe, MJ Yoo, O Gaide, TS Kupper Dermal ?� T Cells Do Not Freely Re-Circulate Out of Skin and Produce IL-17 to Promote Neutrophil Infiltration during Primary Contact Hypersensitivity PLoS ONE, 2017;12(1):e0169397. 2017 [PMID: 28081153] (Flow,, Mouse) Flow Mouse
Sepsis-induced thymic atrophy is associated with defects in early lymphopoiesis Stem Cells, 2016;0(0):. 2016 [PMID: 27422171] (Bioassay, Mouse) Bioassay Mouse
Platelets Guide Leukocytes to Their Sites of Extravasation PLoS Biol, 2016;14(5):e1002459. 2016 [PMID: 27152726] (Bioassay, Mouse) Bioassay Mouse
Velazquez F, Grodecki-Pena A, Knapp A, Salvador A, Nevers T, Croce K, Alcaide P CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo. J Immunol, 2016;196(3):1305-16. 2016 [PMID: 26700769] (Bioassay, Mouse) Bioassay Mouse
Nussbaum C, Bannenberg S, Keul P, Graler M, Goncalves-de-Albuquerque C, Korhonen H, von Wnuck Lipinski K, Heusch G, de Castro Faria Neto H, Rohwedder I, Gothert J, Prasad V, Haufe G, Lange-Sperandio B, Offermanns S, Sperandio M, Levkau B Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin. Nat Commun, 2015;6(0):6416. 2015 [PMID: 25832730] (Bioassay, Mouse) Bioassay Mouse
Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo. Nat Commun, 2015;6(0):6387. 2015 [PMID: 25824568] (Bioassay, Mouse) Bioassay Mouse
Woollard K, Lumsden N, Andrews K, Aprico A, Harris E, Irvine J, Jefferis A, Fang L, Kanellakis P, Bobik A, Chin-Dusting J Raised soluble P-selectin moderately accelerates atherosclerotic plaque progression. PLoS ONE, 2014;9(5):e97422. 2014 [PMID: 24846287] (In Vivo, Mouse) In Vivo Mouse
Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S Endothelial cell-derived chemerin promotes dendritic cell transmigration. J Immunol, 2014;192(5):2366-73. 2014 [PMID: 24470498] (Bioassay, Mouse) Bioassay Mouse
Show All 21 Publications.

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Bioinformatics

Gene Symbol Selp
Uniprot