Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated Recombinant Mouse NTB-A in a functional ELISA. Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
• Source: Mouse myeloma cell line, NS0-derived mouse NTB-A/SLAMF6 protein
  

  Mouse NTB-A (Glu31 - Asn239) Accession # Q9ET39	IEGRMD	Mouse IgG2a (Glu98 - Lys330)
  N-terminus		C-terminus

• Accession #: Q9ET39
• N-terminal Sequence: Glu31
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 50.0 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 66-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF

• Activating NK receptor
• CD352 antigen
• CD352
• KALIFLJ50657
• Ly108
• NK-T-B-antigen
• NTBA receptor
• NTBA
• NTB-A
• NTB-AMGC104953
• NTBAT- and B-cell antigen
• SF2000
• SLAM family member 6
• SLAMF6

Background

NTB-A, also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature mouse NTB-A consists of a 209 amino acid (aa) ECD with one Ig-like V-type and one Ig-like C2-type domain, a 23 aa transmembrane segment, and an 89 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2). Within the ECD, mouse NTB-A shares 48% and 70% aa sequence identity with human and rat NTB-A, respectively. The ECD of mouse NTB-A shares 20% - 34% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. An alternatively spliced isoform diverges after the second ITSM (2). NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (3 - 5). It interacts homophilically through weak associations between the Ig-V type domains (5 - 7). NTB-A functions as an activating coreceptor on NK and T cells (3, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of NK cell IFN-gamma (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11). The isoform with the divergent C-terminal tail is overexpressed in B cells from lupus-prone mice (11).

1. Veillette, A. (2006) Immunol. Rev. 214:22.
2. Peck, S.R. and H.E. Ruley (2000) Immunogenetics 52:63.
3. Bottino, C. et al. (2001) J. Exp. Med. 194:235.
4. Munitz, A. et al. (2005) J. Immunol. 174:110.
5. Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
6. Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
7. Cao, E. et al. (2006) Immunity 25:559.
8. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
9. Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
10. Howie, D. et al. (2005) J. Immunol. 174:5931.
11. Kumar, K.R. et al. (2006) Science 312:1665.

Publications for NTB-A/SLAMF6/CD352 (3986-NT)(2)

Publications using 3986-NT

• Y Baglaenko, M Cruz Tleug, E Gracey, N Talaei, KP Manion, NH Chang, DM Ferri, T Mallevaey, JE Wither Invariant NKT Cell Activation Is Potentiated by Homotypic trans-Ly108 Interactions J. Immunol., 2017-04-03;0(0):. 2017-04-03 [PMID: 28373584] (Bioassay, Mouse)
• Dutta , Mala, Kraus , Zachary, Gomez-Rodriguez , Julio, Hwang , Sun-Hee, Cannons , Jennifer, Cheng , Jun, Lee , Sang-Yun, Wiest , David L, Wakeland , Edward K, Schwartzberg , Pamela L A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes. J Immunol, 2013-01-25;190(5):2121-8. 2013-01-25 [PMID: 23355739] (Neutralization, Mouse)

Bioinformatics

• Uniprot:
  • Q9ET39()