Recombinant Mouse NKG2D Fc Chimera Protein, CF Summary
Details of Functionality |
Measured by its binding ability in a functional ELISA. Immobilized rmNKG2D/Fc Chimera at 4 µg/mL (100 µL/well) can bind biotinylated rmRae-1 gamma /Fc Chimera with a linear range of 1.56-100 ng/mL. |
Source |
Mouse myeloma cell line, NS0-derived mouse NKG2D/CD314 protein MD | Human IgG 1 (Pro100 - Lys330) | IEGR | Mouse NKG2D (Phe94 - Val232) Accession # Q4FJM0 | N-terminus | | | C-terminus | |
Accession # |
|
N-terminal Sequence |
Met |
Structure / Form |
Disulfide-linked homodimer |
Protein/Peptide Type |
Recombinant Proteins |
Gene |
Klrk1 |
Purity |
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
42.7 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
55-60 kDa, reducing conditions |
Publications |
Read Publications using 139-NK in the following applications:
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Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions |
Reconstitute at 100 μg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse NKG2D Fc Chimera Protein, CF
Background
NKG2D is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co-stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha , beta , gamma , δ and epsilon . All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta -2 microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection.
In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1 - 5).
- Bauer, S. et al. (1999) Science 285:727.
- Wu, J. et al. (1999) Science 285:730.
- Diefenbach, A. et al. (2001) Nature 413:165.
- Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
- NKG2D and its Ligands, www.RnDSystems.com.
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FAQs for NKG2D/CD314 (139-NK) (0)