Recombinant Mouse Neurocan Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Neurocan Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.

  The ED₅₀ for this effect is ≤ 0.400 μg/mL.

• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Neurocan protein
  Asp23-Asp637, with a C-terminal 10-His tag
• Accession #: P55066
• N-terminal Sequence: Asp23
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ncan
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 67.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-200 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Neurocan Protein, CF

• chondroitin sulfate proteoglycan 3 (neurocan)
• Chondroitin sulfate proteoglycan 3
• CSPG3
• CSPG3neurocan core protein
• FLJ44681
• NCAN
• NEUR
• neurocan proteoglycan
• Neurocan

Background

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37 - 157), two Link domains (aa 159 - 254 and 258 - 356), two EGF-like domains (aa 960 - 996 and 998 - 1034), one C-type lectin-like domain (aa 1036 - 1165), one Sushi domain (aa 1165 - 1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2 - 3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).

1. Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350.
2. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
3. Retzler, C. et al. (1996) J. Biol. Chem. 271:27304.
4. Quaglia, X. et al. (2008) Brain 131:240.

Publications for Neurocan (5800-NC)(4)

Publications using 5800-NC

• Nakajima, C;Sawada, M;Umeda, E;Takagi, Y;Nakashima, N;Kuboyama, K;Kaneko, N;Yamamoto, S;Nakamura, H;Shimada, N;Nakamura, K;Matsuno, K;Uesugi, S;Vep?ek, NA;Küllmer, F;Nasufovi?, V;Uchiyama, H;Nakada, M;Otsuka, Y;Ito, Y;Herranz-Pérez, V;García-Verdugo, JM;Ohno, N;Arndt, HD;Trauner, D;Tabata, Y;Igarashi, M;Sawamoto, K; Identification of the growth cone as a probe and driver of neuronal migration in the injured brain Nature communications 2024-03-09 [PMID: 38461182] (Bioassay, Mouse)
• T Fontanil, Y Mohamedi, A Moncada-Pa, T Cobo, JA Vega, JL Cobo, O García-Suá, J Cobo, ÁJ Obaya, S Cal Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies Cell. Physiol. Biochem., 2019-01-01;52(5):1003-1016. 2019-01-01 [PMID: 30977985] (Bioassay, Mouse)
• CS Sullivan, I Gotthard, EV Wyatt, S Bongu, V Mohan, RJ Weinberg, PF Maness Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons Sci Rep, 2018-04-18;8(1):6143. 2018-04-18 [PMID: 29670169] (Bioassay, Human)
• Sengottuvel V, Leibinger M, Pfreimer M, Andreadaki A, Fischer D Taxol facilitates axon regeneration in the mature CNS. J. Neurosci., 2011-02-16;31(7):2688-99. 2011-02-16 [PMID: 21325537] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Ncan
• Uniprot:
  • P55066()