Recombinant Mouse NCAM-1/CD56 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse NCAM-1/CD56 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. When 5 x 10⁴ cells/well are added to mouse NCAM-1 coated plates (25 µg/mL with 100 µL/well), >20% will adhere after 1 hour incubation at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse NCAM-1/CD56 protein
  Met1-Thr711, with a C-terminal 6-His tag
• Accession #: NP_001106675
• N-terminal Sequence: Leu20
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ncam1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 77.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-140 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NCAM-1/CD56 Protein, CF

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

Neural cell adhesion molecule 1 (NCAM-1; also CD56) is a membrane-bound glycoprotein that plays an important role in nervous system development and function (1, 2). Mature mouse NCAM-1 consists of a 692 amino acid (aa) extracellular domain (ECD) with five tandem Ig-like domains and two fibronectin type III domains, an 18 aa transmembrane segment, and a 386 aa cytoplasmic domain (3). Three major splice variants of NCAM-1 are expressed: the 180 kDa full length NCAM-180 isoform, the 140 kDa NCAM-140 isoform which lacks most of the cytoplasmic domain, and the 120 kDa GPI-anchored NCAM-120 isoform that includes the ECD only. Splicing is tissue specific and developmentally regulated (4 - 6). Within the ECD, mouse NCAM-1 shares 94% and 95% aa sequence identity with human and rat NCAM-1, respectively. It is expressed on neurons and glial cells, skeletal muscle, and immune NK cells (5, 7 - 9). NCAM-1 is extensively modified with polysialic acid (PSA) during development, but this addition is decreased in adult tissues (5, 6, 8). Polysialylation of NCAM-1 is retained in the adult hippocampus where it is important for synaptic plasticity and memory formation (10). The PSA moiety also participates in the binding of NCAM-1 to heparan sulfate proteoglycans and NCAM-1 mediated migration of olfactory neurons (11, 12). Proteolytic shedding of NCAM-1 liberates a soluble ECD fragment that can inhibit cortical neurite branching and growth (6). The NCAM-140 isoform is preferentially expressed on NK cells that robustly secrete cytokines upon activation (9, 13). Selective up‑regulation of the NCAM-140 isoform in a variety of tumors initiates epithelial-mesenchymal transition (EMT) and promotes tumor cell invasion (14, 15). Finally, NCAM-1 is known to interact with a number of transmembrane and extracellular molecules. NK cell NCAM-1 binds to T cell FGF R1, co-stimulating IL-2 production by T cells (16). NCAM-1 also forms a noncovalent membrane complex with GFR alpha 1, 2 and 4, generating a receptor for GDNF, NTN and PSP, respectively (17). And NCAM-1 is reported to form homophilic trans-interactions, and to interact with L1 CAM in cis, and with HSPGs (agrin and collagen XVIII) in trans. In general, these interactions are involved in cell adhesion, migration, and/or process extension (18).

1. Schmid, R.S. and P.F. Maness (2008) Curr. Opin. Neurobiol. 18:245.
2. Hansen, S.M. et al. (2008) Cell. Mol. Life Sci. 65:3809.
3. Barthels, D. et al. (1987) EMBO J. 6:907.
4. Gennarini, G. et al. (1986) J. Neurosci. 6:1983.
5. Covault, J. et al. (1986) J. Cell Biol. 102:731.
6. Brennaman, L.H. and P.F. Maness (2008) Mol. Cell. Neurosci. 37:781.
7. Noble, M. et al. (1985) Nature 316:725.
8. Chuong, C.M. and G.M. Edelman (1984) J. Neurosci. 4:2354.
9. Lanier, L.L. et al. (1989) J. Exp. Med. 169:2233.
10. Muller, D. et al. (1996) Neuron 17:413.
11. Storms, S.D. and U. Rutishauser (1998) J. Biol. Chem. 273:27124.
12. Tomasiewicz, H. et al. (1993) Neuron 11:1163.
13. Cooper, M.A et al. (2001) Blood 97:3146.
14. Gattenlohner, S. et al. (2009) Am. J. Pathol. 174:1160.
15. Lehembre, F. et al. (2008) EMBO J. 27:2603.
16. Kos, F.J. & Chin, C.S. (2002) Immunol. Cell Biol. 80:364.
17. Paratcha, G. et al. (2003) Cell 113:867.
18. Nielsen, J. et al. (2010) Adv. Exp. Med. Biol. 663:23.

Publications for NCAM-1/CD56 (6070-NC)(1)

Publication using 6070-NC

• Wiertz RW, Marani E, Rutten WL Neural cell-cell and cell-substrate adhesion through N-cadherin, N-CAM and L1. J Neural Eng, 2011-05-31;8(4):046004. 2011-05-31 [PMID: 21628769] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Ncam1
• Uniprot:
  • NP_001106675()