Recombinant Mouse Mimecan Protein, CF

Images

 
There are currently no images for Mimecan/OIF (2949-MC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Mimecan Protein, CF Summary

Details of Functionality
Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. At 15 µg/mL, rmMimecan can significantly retard the rate of type I collagen fibrillogenesis.
Source
Mouse myeloma cell line, NS0-derived mouse Mimecan protein
Ala20-Phe298, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala20
Protein/Peptide Type
Recombinant Proteins
Gene
Ogn
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50 kDa, reducing conditions
Publications
Read Publications using
2949-MC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Mimecan Protein, CF

  • DKFZp586P2421
  • KSPG25
  • mimecan proteoglycan
  • Mimecan
  • OGN
  • OIF
  • OIFOG
  • osteoglycin (osteoinductive factor)
  • osteoglycin
  • Osteoinductive factor
  • SLRR3A
  • SLRR3Acorneal keratan sulfate proteoglycan

Background

Mimecan is a 34 kDa secreted monomeric glycoprotein that belongs to the SLRP, or small leucine-rich proteoglycan family of matrix molecules (1, 2). All SLRP family members contain multiple leucine rich repeats, of which there are two types; a 21 amino acid (aa) proline-containing S-type, and a 26 aa phenylalanine-containing T-type (2, 3). Mimecan is a class III SLRP subfamily member, which means it contains an S-T (vs. S-T-T) repeating motif. Mouse Mimecan is synthesized as a 298 aa precursor that contains a 19 aa signal sequence and a 279 aa mature region (3, 4). The mature region contains a 75 aa N-terminus followed by a 13 aa cysteine-rich region and seven consecutive LRR repeats. There is one (presumably occupied) C-terminal N-linked glycosylation site and a potential keratan sulfate attachment point in LRR #4 (aa 161 - 180) (3). Nomenclature for the mimecan molecule is confusing. Mimecan is best thought of as being the full-length, 279 aa glycoprotein (5). Proteolytic removal of the first 56 aa by BMP-1/Tolloid-like proteinases generates the 25 kDa KSPG form (aa 57 - 279) (3). The 17 kDa, 56 aa mature N-terminus has been referred to as the minecam prosegment (3). When the prosegment is removed, mature mouse KSPG (aa 57 - 279) is 98%, 91%, 90% and 91% aa identical to rat, bovine, human and canine KSPG, respectively. A 12 kDa form (aa 175 - 279) consisting of the 105 aa of the C-terminus (OIF/osteoglycan) has also been observed (6). Noncoding alternate splice forms for mouse are believed to exist and should be expressed in a tissue specific fashion (7, 8). No splice forms are known that impact the coding region. Mimecan seems to exist as proteoglycan (PG) and non-PG forms. In cornea, keratan sulfate is present, although the exact function of the PG is unknown. The corneal extracellular matrix is normally transparent. While mimecan is reported to inhibit fibrillogenesis, possibly by limiting lateral accretion of collagen monomers, it is unclear if the mimican PG actually contributes to transparency (3, 9). In other tissues, mimecan and its derivatives can exist as simple glycoproteins, absent of any PG adduct (6). The uncertain nature of mimecan’s structure and function gives rise to its name: Mime is a Norse-legend dwarf that was notoriously deceitful and misleading (6).

  1. Iozzo, R.V. (1999) J. Biol. Chem. 275:18843.
  2. Matsushima, N. et al. (2000) Proteins 38:210.
  3. Ge, G. et al. (2004) J. Biol. Chem. 279:41626.
  4. Ujita, M. et al. (1995) Gene 158:237.
  5. Hu, S-M. et al. (2005) J. Clin. Endocrinol. Metab. 90:6657
  6. Funderburgh, J.L. et al. (1997) J. Biol. Chem. 272:28089.
  7. Tasheva, E.S. et al. (1999) J. Biol. Chem. 274:18693.
  8. Tasheva, E.S. et al. (1997) J. Biol. Chem. 272:32551.
  9. Tasheva, E.S. et al. (2002) Mol. Vis. 8:407.

Customers Who Viewed This Item Also Viewed...

AF2846
Species: Hu
Applications: WB, IHC
NBP1-84425
Species: Hu, Mu
Applications: IHC, IHC-P
AF143
Species: Hu
Applications: WB, Simple Western, IHC
113-BP/CF
Species: Hu
MAB3110
Species: Hu
Applications: WB, ICC
355-BM/CF
Species: Hu, Mu, Rt
355-BM/CF
Species: Hu, Mu, Rt
AF3308
Species: Mu
Applications: WB
MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
AF5945
Species: Hu, Mu, Rt
Applications: WB
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-38770
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-77461
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-32550
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF1067
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-1514
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, PEP-ELISA, ICC
2949-MC
Species: Mu
Applications: Bioactivity

Publications for Mimecan/OIF (2949-MC)(3)

Reviews for Mimecan/OIF (2949-MC) (0)

There are no reviews for Mimecan/OIF (2949-MC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Mimecan/OIF (2949-MC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Albumin Antibody
NB600-41532
BMP-2 [Unconjugated]
355-BM-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Mimecan Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Ogn
Uniprot