Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. When immobilized as a 3 μL droplet containing 200 ng on a nitrocellulose coated microplate, recombinant mouse MAF Fc Chimera is able to significantly inhibit neurite outgrowth.
Source
Mouse myeloma cell line, NS0-derived mouse MAG/Siglec-4a protein
Mouse MAG/Siglec-4a
(Gly20-Pro516)
Accession # P20917
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Mag
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
82 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
104-124 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein, CF

  • GMAsialic acid binding Ig-like lectin 4A
  • MAG
  • myelin associated glycoprotein
  • myelin-associated glycoprotein
  • sialic acid-binding immunoglobulin-like lectin 4A
  • Siglec4a
  • Siglec-4a
  • S-MAG

Background

Myelin-Associated Glycoprotein (MAG), also known as Siglec-4a, is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily (1). It is composed of an extracellular segment containing five Ig-like domains, a single transmembrane segment, and a cytoplasmic domain. Mature mouse MAG exists as two isoforms, termed S-MAG (short) and L-MAG (long), due to alternative splicing of the cytoplasmic domain (1, 2). S-MAG has a predicted molecular weight of 67 kDa while L-MAG has a predicted molecular weight of 71 kDa (1, 2). Additionally, proteolytic cleavage of the extracellular domain produces a soluble MAG (3). Within shared regions in the extracellular domain, mouse MAG shares 95% and 99% aa sequence identity with human and rat MAG, respectively.  

MAG functions as an adhesion molecule during neural development. It preferentially binds to alpha -2,3-linked sialic acid terminal structures found on cell surface molecules (1, 4, 5). MAG is selectively expressed by myelinating oligodendrocytes and Schwann cells and plays an important role in axon-myelin stability (1, 4). Specifically, L-MAG is involved in myelination in the central nervous system (CNS) while S-MAG is the predominant isoform expressed during myelination in the peripheral nervous system (1). MAG is also reported to regulate the axon cytoskeleton and support the distribution of axon molecules at the nodes of Ranvier (1, 4). In addition, it has been identified as a major inhibitor of neurite outgrowth (1, 4, 6). However, MAG has also been reported to protect neurons from excitotoxicity (1, 7). MAG is believed to utilize the gangliosides GD1a and GT1b, the Nogo receptors NgR1 and NgR2/NgRH1, Integrin beta 1/CD29, and PIR-B to mediate its effects (1, 4, 5, 8, 9). Soluble MAG, which is released from myelin in large quantities, has been identified in normal human tissues and in tissues from patients with neurological disorders (3). It is believed that this soluble MAG might contribute to the lack of CNS neuron regeneration after injury (3).

  1. Lopez, P.H. (2014) Adv. Neurobiol. 9:245.
  2. Salzer, J.L. et al. (1987) J. Cell Biol. 104:957.
  3. Tang, S. et al. (1997) Mol. Cell. Neurosci. 9:333.
  4. Schnaar, R.L. and P.H. Lopez (2009) J. Neurosci. Res. 87:3267.
  5. Schnaar, R.L. (2010) FEBS Lett. 584:1741.
  6. Akbik, F. et al. (2012) Exp. Neurol. 235:43.
  7. Lopez, P.H. et al. (2011) J. Neurochem. 116:900.
  8. Atwal, J.K. et al. (2008) Science 322:967.
  9. Goh, E.L. et al. (2008) Mol. Brain 1:10.

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Bioinformatics

Gene Symbol Mag
Uniprot