Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein, CF


There are currently no images for MAG/Siglec-4a (8580-MG).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. When immobilized as a 3 μL droplet containing 200 ng on a nitrocellulose coated microplate, recombinant mouse MAF Fc Chimera is able to significantly inhibit neurite outgrowth.
Mouse myeloma cell line, NS0-derived mouse MAG/Siglec-4a protein
Mouse MAG/Siglec-4a
Accession # P20917
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
82 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
104-124 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein, CF

  • GMAsialic acid binding Ig-like lectin 4A
  • MAG
  • myelin associated glycoprotein
  • myelin-associated glycoprotein
  • sialic acid-binding immunoglobulin-like lectin 4A
  • Siglec4a
  • Siglec-4a
  • S-MAG


Myelin-Associated Glycoprotein (MAG), also known as Siglec-4a, is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily (1). It is composed of an extracellular segment containing five Ig-like domains, a single transmembrane segment, and a cytoplasmic domain. Mature mouse MAG exists as two isoforms, termed S-MAG (short) and L-MAG (long), due to alternative splicing of the cytoplasmic domain (1, 2). S-MAG has a predicted molecular weight of 67 kDa while L-MAG has a predicted molecular weight of 71 kDa (1, 2). Additionally, proteolytic cleavage of the extracellular domain produces a soluble MAG (3). Within shared regions in the extracellular domain, mouse MAG shares 95% and 99% aa sequence identity with human and rat MAG, respectively.  

MAG functions as an adhesion molecule during neural development. It preferentially binds to alpha -2,3-linked sialic acid terminal structures found on cell surface molecules (1, 4, 5). MAG is selectively expressed by myelinating oligodendrocytes and Schwann cells and plays an important role in axon-myelin stability (1, 4). Specifically, L-MAG is involved in myelination in the central nervous system (CNS) while S-MAG is the predominant isoform expressed during myelination in the peripheral nervous system (1). MAG is also reported to regulate the axon cytoskeleton and support the distribution of axon molecules at the nodes of Ranvier (1, 4). In addition, it has been identified as a major inhibitor of neurite outgrowth (1, 4, 6). However, MAG has also been reported to protect neurons from excitotoxicity (1, 7). MAG is believed to utilize the gangliosides GD1a and GT1b, the Nogo receptors NgR1 and NgR2/NgRH1, Integrin beta 1/CD29, and PIR-B to mediate its effects (1, 4, 5, 8, 9). Soluble MAG, which is released from myelin in large quantities, has been identified in normal human tissues and in tissues from patients with neurological disorders (3). It is believed that this soluble MAG might contribute to the lack of CNS neuron regeneration after injury (3).

  1. Lopez, P.H. (2014) Adv. Neurobiol. 9:245.
  2. Salzer, J.L. et al. (1987) J. Cell Biol. 104:957.
  3. Tang, S. et al. (1997) Mol. Cell. Neurosci. 9:333.
  4. Schnaar, R.L. and P.H. Lopez (2009) J. Neurosci. Res. 87:3267.
  5. Schnaar, R.L. (2010) FEBS Lett. 584:1741.
  6. Akbik, F. et al. (2012) Exp. Neurol. 235:43.
  7. Lopez, P.H. et al. (2011) J. Neurochem. 116:900.
  8. Atwal, J.K. et al. (2008) Science 322:967.
  9. Goh, E.L. et al. (2008) Mol. Brain 1:10.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Rt
Applications: WB
Species: Hu, Mu, Rt, Po, Bv, Ch, GP, Ma, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
Species: Hu
Applications: IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Mu
Applications: WB
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Sh
Applications: WB, Simple Western, ICC/IF, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MAG/Siglec-4a (8580-MG) (0)

There are no publications for MAG/Siglec-4a (8580-MG).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MAG/Siglec-4a (8580-MG) (0)

There are no reviews for MAG/Siglec-4a (8580-MG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for MAG/Siglec-4a (8580-MG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for MAG/Siglec-4a (8580-MG)

Discover related pathways, diseases and genes to MAG/Siglec-4a (8580-MG). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MAG/Siglec-4a (8580-MG)

Discover more about diseases related to MAG/Siglec-4a (8580-MG).

Pathways for MAG/Siglec-4a (8580-MG)

View related products by pathway.

PTMs for MAG/Siglec-4a (8580-MG)

Learn more about PTMs related to MAG/Siglec-4a (8580-MG).

Blogs on MAG/Siglec-4a

There are no specific blogs for MAG/Siglec-4a, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GFAP Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein, CF and receive a gift card or discount.


Gene Symbol Mag