Recombinant Mouse M-CSF Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse M-CSF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5‑3 ng/mL.
Source
E. coli-derived mouse M-CSF protein
Lys33-Glu262, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Csf1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
26 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
29 kDa, reducing conditions
Publications
Read Publications using
416-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse M-CSF Protein, CF

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M‑CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

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Publications for M-CSF (416-ML/CF)(271)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Feline.

We have publications tested in 3 applications: Bioassay, In Vivo, cell culture.


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Bioassay
(261)
In Vivo
(1)
cell culture
(3)
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Human
(2)
Mouse
(262)
Rat
(1)
Feline
(1)
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Showing Publications 1 - 10 of 271. Show All 271 Publications.
Publications using 416-ML/CF Applications Species
M Ahn, DE Anderson, Q Zhang, CW Tan, BL Lim, K Luko, M Wen, WN Chia, S Mani, LC Wang, JHJ Ng, RM Sobota, CA Dutertre, F Ginhoux, ZL Shi, AT Irving, LF Wang Dampened NLRP3-mediated inflammation in bats and implications for a special viral reservoir host Nat Microbiol, 2019;0(0):. 2019 [PMID: 30804542] (Bioassay, Mouse) Bioassay Mouse
V Aguayo, BN Valdés Fer, M Rodríguez-, C Ruiz-Jimén, MJ Ramos-Bení, LB Méndez, AM Espino Fasciola hepatica GST downregulates NF-?B pathway effectors and inflammatory cytokines while promoting survival in a mouse septic shock model Sci Rep, 2019;9(1):2275. 2019 [PMID: 30783117] (Bioassay, Mouse) Bioassay Mouse
TS Kim, YB Jin, YS Kim, S Kim, JK Kim, HM Lee, HW Suh, JH Choe, YJ Kim, BS Koo, HN Kim, M Jung, SH Lee, DK Kim, C Chung, JW Son, JJ Min, JM Kim, CX Deng, HS Kim, SR Lee, EK Jo SIRT3 promotes antimycobacterial defenses by coordinating mitochondrial and autophagic functions Autophagy, 2019;0(0):20-Jan. 2019 [PMID: 30774023] (Bioassay, Mouse) Bioassay Mouse
X Rao, S Zhao, Z Braunstein, H Mao, M Razavi, L Duan, Y Wei, AC Toomey, S Rajagopala, J Zhong Oxidized LDL upregulates macrophage DPP4 expression via TLR4/TRIF/CD36 pathways EBioMedicine, 2019;0(0):. 2019 [PMID: 30738832] (Bioassay, Mouse) Bioassay Mouse
B Zhu, Y Yu, X Liu, Q Han, Y Kang, L Shi CD200 Modulates S. aureus-Induced Innate Immune Responses Through Suppressing p38 Signaling Int J Mol Sci, 2019;20(3):. 2019 [PMID: 30717437] (Bioassay, Mouse) Bioassay Mouse
Q Sun, B Zhang, W Zhu, W Wei, J Ma, FR Tay A potential therapeutic target for regulating osteoporosis via suppression of osteoclast differentiation J Dent, 2019;0(0):. 2019 [PMID: 30716449] (Bioassay, Mouse) Bioassay Mouse
CL Chan, JY Chen, MC Shih, CA Wang, YM Liou L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts J. Biomed. Sci., 2019;26(1):12. 2019 [PMID: 30678675] (Bioassay, Mouse) Bioassay Mouse
H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury JCI Insight, 2019;0(0):. 2019 [PMID: 30676324] (Bioassay, Mouse) Bioassay Mouse
S Murugan, P Jakka, S Namani, V Mujumdar, G Radhakrish The neurosteroid, pregnenolone promotes degradation of key proteins in the innate immune signalling to suppress inflammation J. Biol. Chem., 2019;0(0):. 2019 [PMID: 30647133] (Bioassay, Mouse) Bioassay Mouse
NKY Wee, BP Sinder, S Novak, X Wang, C Stoddard, BG Matthews, I Kalajzic Skeletal phenotype of the neuropeptide Y knockout mouse Neuropeptides, 2019;73(0):78-88. 2019 [PMID: 30522780] (Bioassay, Mouse) Bioassay Mouse
Show All 271 Publications.

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Blogs on M-CSF.

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Bioinformatics

Gene Symbol Csf1
Uniprot