Recombinant Mouse M-CSF Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse M-CSF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5‑3 ng/mL.
Source
E. coli-derived mouse M-CSF protein
Lys33-Glu262, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Csf1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
26 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
29 kDa, reducing conditions
Publications
Read Publications using
416-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse M-CSF Protein, CF

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M‑CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

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Publications for M-CSF (416-ML/CF)(244)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Feline.

We have publications tested in 3 applications: Bioassay, In Vivo, cell culture.


Filter By Application
Bioassay
(235)
In Vivo
(1)
cell culture
(3)
All Applications
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Human
(1)
Mouse
(237)
Rat
(1)
Feline
(1)
All Species
Showing Publications 1 - 10 of 244. Show All 244 Publications.
Publications using 416-ML/CF Applications Species
Subramanian K, Neill D, Malak H, Spelmink L, Khandaker S, Dalla Libera Marchiori G, Dearing E, Kirby A, Yang M, Achour A, Nilvebrant J, Nygren P, Plant L, Kadioglu A, Henriques-Normark B Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nat Microbiol, 0;0(0):. 0 [PMID: 30420782] (Bioassay, Mouse) Bioassay Mouse
X Su, L Yang, Y Yin, J Huang, F Qiao, Y Fang, L Yu, Y Wang, K Zhou, J Wang Bone marrow mesenchymal stem cells tune the differentiation of myeloid-derived suppressor cells in bleomycin-induced lung injury Stem Cell Res Ther, 2018;9(1):253. 2018 [PMID: 30257700] (Bioassay, Mouse) Bioassay Mouse
E Mobarak, L Håversen, M Manna, M Rutberg, M Levin, R Perkins, T Rog, I Vattulaine, J Borén Glucosylceramide modifies the LPS-induced inflammatory response in macrophages and the orientation of the LPS/TLR4 complex in silico Sci Rep, 2018;8(1):13600. 2018 [PMID: 30206272] (Bioassay, Mouse) Bioassay Mouse
Y Ikebuchi, S Aoki, M Honma, M Hayashi, Y Sugamori, M Khan, Y Kariya, G Kato, Y Tabata, JM Penninger, N Udagawa, K Aoki, H Suzuki Coupling of bone resorption and formation by RANKL reverse signalling Nature, 2018;0(0):. 2018 [PMID: 30185903] (Bioassay, Mouse) Bioassay Mouse
X Yi, J Zhang, R Zhuang, S Wang, S Cheng, D Zhang, J Xie, W Hu, X Liu, Y Zhang, Y Ding, Y Zhang Silencing LAIR-1 in human THP-1 macrophage increases foam cell formation by modulating PPAR? and M2 polarization Cytokine, 2018;111(0):194-205. 2018 [PMID: 30176557] (Bioassay, Mouse) Bioassay Mouse
Y Zur, L Rosenfeld, CA Keshelman, N Dalal, G Guterman-R, A Orenbuch, Y Einav, N Levaot, N Papo A dual-specific macrophage colony-stimulating factor antagonist of c-FMS and ?v?3 integrin for osteoporosis therapy PLoS Biol., 2018;16(8):e2002979. 2018 [PMID: 30142160] (Bioassay, Mouse) Bioassay Mouse
MA Ferko, I Catelas Effects of metal ions on caspase-1 activation and interleukin-1? release in murine bone marrow-derived macrophages PLoS ONE, 2018;13(8):e0199936. 2018 [PMID: 30138321] (Bioassay, Mouse) Bioassay Mouse
G Locatelli, D Theodorou, A Kendirli, MJC Jordão, O Staszewski, K Phulphagar, L Cantuti-Ca, A Dagkalis, A Bessis, M Simons, F Meissner, M Prinz, M Kerschenst Mononuclear phagocytes locally specify and adapt their phenotype in a multiple sclerosis model Nat. Neurosci., 2018;21(9):1196-1208. 2018 [PMID: 30127427] (Bioassay, Mouse) Bioassay Mouse
G Melagraki, E Ntougkos, D Papadopoul, V Rinotas, G Leonis, E Douni, A Afantitis, G Kollias In Silico Discovery of Plant-Origin Natural Product Inhibitors of Tumor Necrosis Factor (TNF) and Receptor Activator of NF-?B Ligand (RANKL) Front Pharmacol, 2018;9(0):800. 2018 [PMID: 30090063] (Bioassay, Mouse) Bioassay Mouse
C McCarthy, E Lee, JP Bridges, A Sallese, T Suzuki, JC Woods, BJ Bartholmai, T Wang, C Chalk, BC Carey, P Arumugam, K Shima, EJ Tarling, BC Trapnell Statin as a novel pharmacotherapy of pulmonary alveolar proteinosis Nat Commun, 2018;9(1):3127. 2018 [PMID: 30087322] (Bioassay, Mouse) Bioassay Mouse
Show All 244 Publications.

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Bioinformatics

Gene Symbol Csf1
Uniprot