Recombinant Mouse M-CSF Protein, CF


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Recombinant Mouse M-CSF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5‑3 ng/mL.
E. coli-derived mouse M-CSF protein
Lys33-Glu262, with an N-terminal Met
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
26 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
29 kDa, reducing conditions
Read Publications using
416-ML/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse M-CSF Protein, CF

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930


M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M‑CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

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Publications for M-CSF (416-ML/CF)(271)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Feline.

We have publications tested in 3 applications: Bioassay, In Vivo, cell culture.

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Showing Publications 1 - 10 of 271. Show All 271 Publications.
Publications using 416-ML/CF Applications Species
M Ahn, DE Anderson, Q Zhang, CW Tan, BL Lim, K Luko, M Wen, WN Chia, S Mani, LC Wang, JHJ Ng, RM Sobota, CA Dutertre, F Ginhoux, ZL Shi, AT Irving, LF Wang Dampened NLRP3-mediated inflammation in bats and implications for a special viral reservoir host Nat Microbiol, 2019;0(0):. 2019 [PMID: 30804542] (Bioassay, Mouse) Bioassay Mouse
V Aguayo, BN Valdés Fer, M Rodríguez-, C Ruiz-Jimén, MJ Ramos-Bení, LB Méndez, AM Espino Fasciola hepatica GST downregulates NF-?B pathway effectors and inflammatory cytokines while promoting survival in a mouse septic shock model Sci Rep, 2019;9(1):2275. 2019 [PMID: 30783117] (Bioassay, Mouse) Bioassay Mouse
TS Kim, YB Jin, YS Kim, S Kim, JK Kim, HM Lee, HW Suh, JH Choe, YJ Kim, BS Koo, HN Kim, M Jung, SH Lee, DK Kim, C Chung, JW Son, JJ Min, JM Kim, CX Deng, HS Kim, SR Lee, EK Jo SIRT3 promotes antimycobacterial defenses by coordinating mitochondrial and autophagic functions Autophagy, 2019;0(0):20-Jan. 2019 [PMID: 30774023] (Bioassay, Mouse) Bioassay Mouse
X Rao, S Zhao, Z Braunstein, H Mao, M Razavi, L Duan, Y Wei, AC Toomey, S Rajagopala, J Zhong Oxidized LDL upregulates macrophage DPP4 expression via TLR4/TRIF/CD36 pathways EBioMedicine, 2019;0(0):. 2019 [PMID: 30738832] (Bioassay, Mouse) Bioassay Mouse
B Zhu, Y Yu, X Liu, Q Han, Y Kang, L Shi CD200 Modulates S. aureus-Induced Innate Immune Responses Through Suppressing p38 Signaling Int J Mol Sci, 2019;20(3):. 2019 [PMID: 30717437] (Bioassay, Mouse) Bioassay Mouse
Q Sun, B Zhang, W Zhu, W Wei, J Ma, FR Tay A potential therapeutic target for regulating osteoporosis via suppression of osteoclast differentiation J Dent, 2019;0(0):. 2019 [PMID: 30716449] (Bioassay, Mouse) Bioassay Mouse
CL Chan, JY Chen, MC Shih, CA Wang, YM Liou L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts J. Biomed. Sci., 2019;26(1):12. 2019 [PMID: 30678675] (Bioassay, Mouse) Bioassay Mouse
H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury JCI Insight, 2019;0(0):. 2019 [PMID: 30676324] (Bioassay, Mouse) Bioassay Mouse
S Murugan, P Jakka, S Namani, V Mujumdar, G Radhakrish The neurosteroid, pregnenolone promotes degradation of key proteins in the innate immune signalling to suppress inflammation J. Biol. Chem., 2019;0(0):. 2019 [PMID: 30647133] (Bioassay, Mouse) Bioassay Mouse
NKY Wee, BP Sinder, S Novak, X Wang, C Stoddard, BG Matthews, I Kalajzic Skeletal phenotype of the neuropeptide Y knockout mouse Neuropeptides, 2019;73(0):78-88. 2019 [PMID: 30522780] (Bioassay, Mouse) Bioassay Mouse
Show All 271 Publications.

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Gene Symbol Csf1