Recombinant Mouse Kirrel1/NEPH1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Kirrel1/NEPH1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of MS‑1 mouse pancreatic islet endothelial cells. When 5 x 10⁴ cells/well are added to rmKirrel1 coated plates (30 µg/mL, 100 µg/well), approximately 60%-80% will adhere after 90 minutes at 37° C.
• Source: Mouse myeloma cell line, NS0-derived mouse Kirrel1/NEPH1 protein
  Leu48-Leu525, with a C-terminal 6-His tag
• Accession #: Q80W68
• N-terminal Sequence: Leu48
• Protein/Peptide Type: Recombinant Proteins
• Gene: Kirrel
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 53.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-77 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Kirrel1/NEPH1 Protein, CF

• FLJ10845
• kin of IRRE like (Drosophila)
• KIRREL
• Kirrel1
• MGC129542
• MGC129543
• NEPH1
• nephrin-like protein 1

Background

Kirrel1, also called neph1, is a 90 - 110 kDa type I transmembrane glycoprotein that belongs to the NEPH family of the immunoglobulin superfamily (1 - 4). The 789 amino acid (aa) mouse Kirrel1 contains a 47 aa signal sequence, a 484 aa extracellular domain (ECD) with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 237 aa cytoplasmic domain (5). An isoform diverges in the cytoplasmic domain and ends at aa 634, prior to four tyrosine phosphorylation sites, a podocin interaction motif, and a C-terminal PDZ motif that are all important for the signaling functions of Kirrel1 (1, 6). If expressed, this isoform would be expected to block homo- and hetero-multimer formation, thus acting as an inhibitor. The ECD also contains a site for FGF/FGF R interaction, and an RGD site that may allow integrin-mediated cell attachment (5). Mouse Kirrel1 shares 99% and 97% aa identity with rat and human Kirrel1, respectively, within the ECD. Kirrel1 expression has been mainly studied in the kidney glomerular slit diaphragm, but its expression with nephrin or other family members has also been reported in central nervous system neurons, pancreas and placenta (3, 4, 7 - 9). Kirrel1 forms cis hetero-oligomers with nephrin, which brings together signaling molecules that direct actin polymerization (3, 4, 10). This interaction is essential for barrier function in the slit diaphragm, and mice deleted for Kirrel1 die perinatally due to proteinuria and failure to thrive (2, 3).

1. Sellin, L. et al. (2003) FASEB J.17:115.
2. Donoviel, D.B. et al. (2001) Mol. Cell. Biol. 21:4829.
3. Liu, G. et al. (2003) J. Clin. Invest. 112:209.
4. Barletta, G-M. et al. (2003) J. Biol. Chem. 278:19266.
5. Swissprot Accession # Q96J84.
6. Huber T.B. et al. (2003) J. Biol. Chem. 278:13417.
7. Gerke, P. et al. (2006) J. Comp. Neurol. 498:466.
8. Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
9. Beall, M.H. et al. (2005) J. Soc. Gynecol. Investig. 12:298.
10. Garg, P. et al. (2007) Mol. Cell. Biol. 27:8698.

Bioinformatics

• Gene Symbol: Kirrel
• Uniprot:
  • Q80W68()