PATJ Antibody

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Immunohistochemistry-Paraffin: PATJ Antibody [NB100-1354] - 3.8ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PATJ Antibody Summary

Immunogen
Peptide with sequence PENPATDKLQVLQ-C corresponding to N-Terminus according to NP_795352.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
INADL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments in human brain and kidney lysates consistently showed a strong band at 100 kDa band followed by bands at ~55 kDa, 45 kDa band and 40 kDa band at 1 ug/ml. Please note that currently we cannot find an explanation in scientific literature for the bands we observe given the predicted MW of approx. 196 kDa band according to NP_795352.2. IHC-P: Human testis shows strong staining of spermatids.
Positive Control
Kidney Lysate (NB820-59231)
Brain Lysate (NB820-59177)
Publications
Read Publication using NB100-1354.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PATJ Antibody

  • channel-interacting PDZ domain protein
  • FLJ26982
  • hINADL
  • inactivation no after-potential D-like protein
  • Inadl protein
  • InaD-like (Drosophila)
  • inaD-like protein
  • InaD-like
  • Pals1-associated tight junction protein
  • PATJCipp
  • PDZ domain protein
  • Protein associated to tight junctions

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PATJ Antibody (NB100-1354)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol INADL
Entrez
Uniprot