Kirrel3/NEPH2 Antibody - BSA Free

Western Blot: Kirrel3/NEPH2 Antibody [NBP2-82000] - Western blot analysis of Kirrel3/NEPH2 in mouse kidney tissue lysate with Kirrel3/NEPH2 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: Kirrel3/NEPH2 Antibody [NBP2-82000] - Immunohistochemistry of Kirrel3/NEPH2 in mouse kidney tissue with Kirrel3/NEPH2 antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Kirrel3/NEPH2 Antibody - BSA Free [NBP2-82000] - Immunofluorescence of Kirrel3/NEPH2 in mouse kidney tissue with Kirrel3/NEPH2 antibody at 20 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Kirrel3/NEPH2 Antibody - BSA Free Summary

• Immunogen: Kirrel3 antibody was raised against a 19 amino acid peptide near the amino terminus of human Kirrel3. The immunogen is located within amino acids 20 - 70 of Kirrel3. Amino Acid Squence: MAKDKFRRMNEGQVYSFSQ
• Specificity: Kirrel3/NEPH2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. Kirrel3/NEPH2 antibody is predicted to not cross-react with other members of the KIRREL protein family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIRREL3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Kirrel3/NEPH2 Antibody - BSA Free

• KIAA1867MGC126824
• kin of IRRE like 3 (Drosophila)
• Kin of irregular chiasm-like protein 3
• kin of IRRE-like protein 3
• KIRRE
• Kirrel3
• KIRREPRO4502
• mKIRRE
• MRD4
• NEPH2
• NEPH2MGC126850
• nephrin-like 2
• Nephrin-like protein 2

Background

NEPH2 is a member of the nephrin-like protein family, which includes NEPH1 (KIRREL; MIM 607428) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIRREL3