Kirrel2/NEPH3 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kirrel2/NEPH3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Kirrel2/NEPH3
  Ser19-Asp501
  Accession # Q7TSU7
• Specificity: Detects mouse Kirrel2/NEPH3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human Kirrel2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KIRREL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kirrel2/NEPH3 Antibody [Unconjugated]

• DKFZp564A1164
• Filtrin
• FILTRINkin of IRRE-like protein 2
• kin of IRRE like 2 (Drosophila)
• Kin of irregular chiasm-like protein 2
• Kirrel2
• MGC15718
• NEPH3
• NEPH3Nephrin-like protein 3
• nephrin-like 3
• nephrin-like gene 1
• NLG1
• NLG1kin of irregular chiasm-like 2

Background

Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin superfamily (1, 2). Kirrel2 is one of several proteins expressed both in pancreatic islet beta cells and kidney glomerulus, and is also found in brain and lymph nodes (1‑9). Mouse Kirrel2 cDNA encodes a 700 amino acid (aa) protein that contains a 16 aa signal sequence, a 491 aa extracellular domain with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 172 aa intracellular domain with a nephrin-like, conserved podocin binding site (mouse aa 589‑598) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of mouse Kirrel2 shares 96%, 86%, 85% and 85% aa identity with rat, human, equine and bovine Kirrel2, respectively. It shares 35‑37% aa identity with other mouse Kirrel proteins. One mouse isoform that has been described is missing the transmembrane domain (aa 501‑533), while another encodes only portions of Ig domains 1‑4 (5). Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (6). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet beta cell function (7). In the brain, Kirrel2 is present in olfactory neurons; but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (8). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (9).

1. Sun C. et al. (2003) Genomics 82:130.
2. Ihalmo, P. et al. (2003) Biochem. Biophys. Res. Commun. 300:364.
3. Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
4. Sellin, L. et al. (2003) FASEB J. 17:115.
5. SwissProt Accession # Q7TSU7.
6. Ihalmo, P. et al. (2007) Nephrol. Dial. Transplant 22:1903.
7. Rinta-Valkama, J. et al. (2007) Diabetes Metab. Res. Rev. 23:119.
8. Serizawa, S. et al. (2006) Cell 127:1057.
9. Minaki, Y. et al. (2005) Neurosci. Res. 52:250.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kirrel2/NEPH3 Antibody (AF2930)(8)

Publications using AF2930

• Jennifer M Lin, Tyler A Mitchell, Megan Rothstein, Alison Pehl, Ed Zandro M Taroc, Raghu R Katreddi et al. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2 epsilon in vomeronasal sensory neurons eLife 9/16/2022 [PMID: 36111787]
• Miguel-Escalada I, Maestro MA, Balboa D et al. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster Developmental cell 8/22/2022 [PMID: 35998583]
  
  Details:
  Supplementary figure 5
• Neelima Vaddadi, Katrine Iversen, Reesha Raja, Alina Phen, Alexandra Brignall, Emilie Dumontier et al. Kirrel2 is differentially required in populations of olfactory sensory neurons for the targeting of axons in the olfactory bulb Development 1/1/2019 [PMID: 31142543]
• Neelima Vaddadi, Katrine Iversen, Reesha Raja, Alina Phen, Alexandra Brignall, Emilie Dumontier et al. Kirrel2 is differentially required in populations of olfactory sensory neurons for the targeting of axons in the olfactory bulb Development 2019-01-01 [PMID: 31142543] (Immunohistochemistry, Transgenic Mouse)
• Aya Murai, Ryo Iwata, Satoshi Fujimoto, Shuhei Aihara, Akio Tsuboi, Yuko Muroyama et al. Distorted Coarse Axon Targeting and Reduced Dendrite Connectivity Underlie Dysosmia after Olfactory Axon Injury eNeuro 10/5/2016 [PMID: 27785463]
• Manjana Saleh, Karsten Jürchott, Sonja Oberland, Eva M. Neuhaus, Achim Kramer, Ute Abraham Genome-Wide Screen Reveals Rhythmic Regulation of Genes Involved in Odor Processing in the Olfactory Epithelium Journal of Biological Rhythms 12/1/2015 [PMID: 26482709]
• Henion T, Madany P, Faden A, Schwarting G beta3GnT2 null mice exhibit defective accessory olfactory bulb innervation. Mol Cell Neurosci, 2012-09-21;52(0):73-86. 2012-09-21 [PMID: 23006775] (IHC-Fr, Mouse)
• Henion TR, Faden AA, Knott TK, Schwarting GA beta3GnT2 maintains adenylyl cyclase-3 signaling and axon guidance molecule expression in the olfactory epithelium. J. Neurosci., 2011-04-27;31(17):6576-86. 2011-04-27 [PMID: 21525298] (IHC-Fr, Western Blot, Mouse)

Review for Kirrel2/NEPH3 Antibody (AF2930) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 12/19/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Olfactory epithelium sections
• Species: Mouse

Bioinformatics

• Gene Symbol: KIRREL2
• Uniprot:
  • Q7TSU7()