Recombinant Mouse Integrin alpha X beta 2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Integrin alpha X beta 2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes. The ED₅₀ for this effect is 0.750-7.50 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha X beta 2 protein
  

  Mouse Integrin alpha X (Phe20-Pro1116) Accession # Q9QXH4	His-Pro	GGGSGGGS	Acidic Tail	6-His tag
  Mouse Integrin beta 2 (Gln24-Asn702) Accession # P11835	His-Pro	GGGSGGGS	Basic Tail
  N-terminus				C-terminus

• Accession #: Q9QXH4 (Integrin alpha X) & P11835 (Integrin beta 2)
• N-terminal Sequence: Phe20 (Integrin alpha X) & Gln24 predicted, No results obtained: sequencing might be blocked (Integrin beta 2)
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 131 kDa (Integrin alpha X) & 83 kDa (Integrin beta 2).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 135-150 kDa & 95-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha X beta 2 Protein, CF

• Integrin alpha X beta 2

Background

Integrin alpha X beta 2, also called CD11c/CD18, p150/95 or complement receptor type 4 (CR4), is one of four beta 2 integrins. The non-covalent heterodimer of 150 kDa alpha X/CD11c and 95 kDa beta 2/CD18 integrin subunits is commonly used as a marker for dendritic cells (DC) and classically activated macrophages (M1), but is also expressed on hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells (1‑7). Inflammation enhances surface expression of alpha X beta 2 in states such as obesity (adipose DC), asthma (lung DC) and hypertriglyceridemia (circulating monocytes), increasing the adhesive capacity of the cells and contributing to pathology (8‑10). The alpha X vWFA or I‑domain, which contains the adhesion sites, forms the N‑terminal head region with the alpha X beta-propeller and the beta 2 vWFA domain (1, 11). Like other integrins, alpha X beta 2 has multiple activation states (3). In the presence of divalent cations and "inside-out" signaling, alpha X beta 2 is fully active and extended. In the inactive state, the heterodimer flexes in the center at the alpha X thigh and calf domains and beta 2 I‑EGF domains, impeding access to adhesion sites (1). The 1097 aa mouse alpha X/CD11c ECD shares 87% aa sequence identity with rat, and 71‑73% with human, canine and equine alpha X, while the 679 aa mouse beta 2/CD18 ECD shares 91% aa sequence identity with rat, and 80-82% with human, bovine, canine, and porcine beta 2 ECD. Active alpha X beta 2 shares some adhesion partners with alpha M beta 2/CD11b/CD18, including complement opsonin fragment iC3b, ICAMs, vWF and fibrinogen, and is expressed on many of the same cells (4-14). However, alpha M beta 2 activity is often constitutive, while alpha X beta 2 activity requires cell activation (4-7). alpha X beta 2 also binds osteopontin, Thy-1, plasminogen, heparin, and proteins with abnormally exposed acidic residues (14-18). The adhesion events are important for proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles (5, 6, 12, 14, 15). Mutations of beta 2, especially in the vWFA domain, cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections (19).

1. Corbi, A.L. et al. (1987) EMBO J. 6:4023.
2. Kishimoto, T.K. et al. (1987) Cell 48:681.
3. Hynes, R.O. (2002) Cell 110:673.
4. Arnaout, M.A. (1990) Blood 75:1037.
5. Postigo, A.A. et al. (1991) J. Exp. Med. 174:1313.
6. Beyer, M. et al. (2005) Respir. Res. 6:70.
7. Nicolaou, F. et al. (2003) Blood 101:4033.
8. Stefanovic-Racic, M. et al. (2012) Diabetes 61:2330.
9. Gower, R.M. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:160.
10. van Rijt, L.S. et al. (2005) J. Exp. Med. 201:981.
11. Vorup-Jensen, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1873.
12. Bilsland, C.A.G. et al. (1994) J. Immunol. 152:4582.
13. Pendu, R. et al. (2006) Blood 108:3746.
14. Sadhu, C. et al. (2007) J. Leukoc. Biol. 81:1395.
15. Schack, L. et al. (2009) J. Immunol. 182:6943.
16. Choi, J. et al. (2005) Biochem. Biophys. Res. Commun. 331:557.
17. Gang, J. et al. (2007) Mol. Cells 24:240.
18. Vorup-Jensen, T. et al. (2007) J. Biol. Chem. 282:30869.
19. Kishimoto, T.K. et al. (1987) Cell 50:193.

Publications for Integrin alpha X beta 2 (7987-AX)(2)

Publications using 7987-AX

• Takeda, K;Kaifu, T;Michihata, R;Kinugawa, N;Fujioka, A;Tateno, A;Toshima, K;Kanoh, H;Inamori, KI;Kamijo, K;Himeda, T;Ohara, Y;Inokuchi, JI;Nakamura, A; Chronic encephalomyelitis virus exhibits cellular tropism and evades pDCs by binding to sialylated integrins as the cell surface receptors European journal of immunology 2023-08-11 [PMID: 37565654] (In Vivo, Mouse)
• D Heinzmann, M Noethel, SV Ungern-Ste, I Mitroulis, M Gawaz, T Chavakis, AE May, P Seizer CD147 is a Novel Interaction Partner of Integrin &amp;alphaM&amp;beta2 Mediating Leukocyte and Platelet Adhesion Biomolecules, 2020-04-02;10(4):. 2020-04-02 [PMID: 32252487] (ELISA Capture, Mouse)

Bioinformatics

• Uniprot:
  • Q9QXH4 (Integrin alpha X) & P11835 (Integrin beta 2)()