Recombinant Mouse Integrin alpha V beta 6 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Integrin alpha V beta 6 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Integrin alpha V beta 6 at 2.0 µg/mL can bind Recombinant Human LAP TGF‑ beta 1 (Catalog # 246-LP) with an apparent K_D <0.1 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha V beta 6 protein
  

  Mouse Integrin alpha V (Phe31-Val988) Accession # P43406	HP	GGGSGGGSGGGS	Acidic Tail	HHHHHH
  Mouse Integrin beta 6 (Gly22-Asn706) Accession # Q9Z0T9		GGGSGGGSGGGS	Basic Tail
  N-terminus				C-terminus

• Accession #: P43406 (Integrin alpha V) & Q9Z0T9 (Integrin beta 6)
• N-terminal Sequence: Phe31 ( alpha V subunit) & Gly22 ( beta 6 subunit)
• Structure / Form: Non-covalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ITGAV
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 115 kDa ( alpha V subunit) & 82.6 kDa ( beta 6 subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 135-150 kDa & 110-125 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha V beta 6 Protein, CF

• CD51
• Integrin alpha V beta 6
• integrin subunit alpha V
• ITGAV
• MSK8
• VNRA
• VTNR

Background

Integrin alpha V beta 6 is one of five alpha V integrins and the sole beta 6 integrin (1, 2). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 95 kDa beta 6 integrin subunits is expressed exclusively on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas (2‑5). The ligand interaction site of alpha V beta 6 is in the N‑terminal head region formed by an interaction of the beta 6 vWFA domain with the alpha V beta‑propeller structure (2). The alpha V subunit contains domains termed thigh, calf, and calf‑2 with a divalent cation‑binding site found at a position equivalent to the “knee”. The 958 aa mouse  alpha V ECD (4), which is cleaved at aa 886 but remains associated, shares 92‑95% aa sequence identity with human and bovine alpha V, while the 687 aa mouse beta 6 ECD (5) shares 90‑96% aa sequence identity with human, rat, bovine, ovine, and porcine  beta 6. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. The beta 6 C‑terminal 11 amino acid (aa) cytoplasmic sequence transduces a signal, enhancing proliferation and inducing MMP‑9 expression (6). Either “inside‑out” signaling or Mg²⁺ or Mn²⁺ binding unfolds and activates the integrin (1). Active alpha V beta 6 binds matrix proteins fibronectin and tenascin C (2). It also binds the TGF‑ beta latency‑associated peptide (LAP) and activates TGF‑ beta 1 or TGF‑ beta 3 from large latent complexes (7). This activation requires interaction with LTBP‑1 and fibronectin, and is enhanced by PAR-1 (8, 9). Deletion of beta 6 ablates tonic inhibition of alveolar macrophages by TGF‑ beta , inhibits intestinal regulatory T cell production, and predisposes mice to inflammatory reactions in the skin, lungs, and intestines where irritations and microbial challenges are frequent (10‑12). High alpha V beta 6 expression in carcinomas may contribute to progression through its effects on TGF‑ beta and MMP activity (3). The foot-and-mouth disease virus and several other viruses can use alpha V beta 6 as a receptor, and soluble alpha V beta 6 may block virus infectivity in vitro (13, 14).

1. Hynes, R.O. (2002) Cell 110:673.
2. Sheppard, D. (2004) Curr. Opin. Cell Biol. 16:552.
3. Bandyopadhyay, A. and S. Raghavan (2009) Curr. Drug Targets 10:645.
4. Wada, J. et al. (1996) J. Cell Biol. 132:1161.
5. Arend, L.J. et al. (2000) J. Am. Soc. Nephrol. 11:2297.
6. Dixit, R.B. et al. (1996) J. Biol. Chem. 271:25976.
7. Munger, J.S. et al. (1999) Cell 96:319.
8. Fontana, L. et al. (2005) FASEB J. 19:1798.
9. Jenkins, R.G. et al. (2006) J. Clin. Invest. 116:1606.
10. Huang, X.Z. et al. (1996) J. Cell Biol. 133:921.
11. Morris, D.G. et al. (2003) Nature 422:169.
12. Chen, X. et al. (2011) J. Leukoc. Biol. 90:751.
13. Berryman, S. et al. (2005) J. Virol. 79:8519.
14. Heikkila, O. et al. (2009) J. Gen. Virol. 90:197.

Publications for Integrin alpha V beta 6 (7480-AV)(1)

Publication using 7480-AV

• M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection PloS Pathogens, 2021-12-15;17(12):e1010083. 2021-12-15 [PMID: 34910784] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: ITGAV
• Uniprot:
  • P43406 (Integrin alpha V) & Q9Z0T9 (Integrin beta 6)()