Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Collagen I is immobilized at 5 μg/mL, Recombinant Mouse Integrin alpha 1 beta 1 can bind with an apparent K_d <5 nM.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 1 beta 1 protein
  

  Mouse Integrin alpha 1 (Phe29-Pro1141) Accession # Q3V3R4	His-Pro	GGGSGGGS	Acidic Tail	6-His tag
  Mouse Integrin beta 1 (Gln21-Asp728) Accession # P09055	His-Pro	GGGSGGGS	Basic Tail
  N-terminus				C-terminus

• Accession #: Q3V3R4(Integrin alpha 1) & P09055 (Integrin beta 1)
• N-terminal Sequence: Phe29 (Integrin alpha 1) & No results obtained: Gln21 predicted (Integrin beta 1), sequencing might be blocked
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 132 kDa (Integrin alpha 1) & 86.5 kDa (Integrin beta 1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-135 kDa & 180-215 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

• Integrin alpha 1 beta 1

Background

Integrin alpha 1 beta 1, also called VLA1, is the only alpha 1 integrin family adhesion receptor, one of twelve integrins that share the beta 1 subunit, and one of four collagen-binding integrins (1-6). It is the non-covalent heterodimer of 190-210 kDa alpha 1 (CD49a) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits. It is found on cells including activated T cells, B cells, monocytes, vascular smooth muscle cells, osteoblasts and adipocytes (2, 7-9). The alpha 1 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 4). The beta 1 ECD contains a vWFA domain, which participates in binding (3). Each subunit then has a transmembrane sequence and a short cytoplasmic tail. Divalent cations and intracellular (inside-out) signaling convert the dimer from the folded, inactive form to its most active, extended conformation (1, 2). The 1113 amino acid (aa) mouse alpha 1 extracellular domain (ECD) shares 88% and 96% aa sequence identity with human and rat alpha 1, respectively, while the 708 aa mouse beta 1 ECD shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. alpha 1 beta 1 preferentially binds collagens I, IV, VI, XIII and XVI, but also binds laminin (4-11). alpha 1 beta 1 is reported to down-regulate EGF R signaling, increase expression of caveolin-1, reduce production of reactive oxygen species, regulate collagen expression, control MMP collagenase and gelatinase activity, and mediate the renal basement membrane disorder Alport syndrome (11-13). These effects may begin by alpha 1 beta 1 binding of caveolin-1, initiating signaling pathways that involve the phosphatase TC-PTP, kinases ERK and p38, and the transcription factor PPAR-gamma (11-14). alpha 1 beta 1 down-regulates MMP-mediated angiostatin formation, enhancing tumor vascularization (9). alpha 1 beta 1-null mice are deficient in fibroblast collagen IV and laminin-mediated cell spreading and migration, show defects in bone healing, and are resistant to Alport renal fibrosis (10-12, 15). When expressed in the same epithelial cells, alpha 1 beta 1 negatively regulates integrin alpha 2 beta 1-mediated cell adhesion and migration (16).

1. Takada, Y. et al. (2007) Genome Biol. 8:215.
2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
4. Briesewitz, R. et al. (1993) J. Biol. Chem. 268:2989.
5. Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.
6. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
7. Eble, J.A. et al. (2006) J. Biol. Chem. 281:25745.
8. Hall, D.E. et al. (1990) J. Cell Biol. 110:2175.
9. Pozzi, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2202.
10. Gardner, H. et al. (1999) J. Cell Sci. 112:263.
11. Dennis, J. et al. (2010) Am. J. Pathol. 177:2527.
12. Cosgrove, D. et al. (2008) Am. J. Pathol. 172:761.
13. Chen, X. et al. (2010) Mol. Cell. Biol. 30:3048.
14. Borza, C.M. et al. (2010) J. Biol. Chem. 285:40114.
15. Gardner, H. et al. (1996) Dev. Biol. 175:301.
16. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.

Publications for Integrin alpha 1 beta 1 (8188-AB)(3)

Publications using 8188-AB

• Takeda, K;Kaifu, T;Michihata, R;Kinugawa, N;Fujioka, A;Tateno, A;Toshima, K;Kanoh, H;Inamori, KI;Kamijo, K;Himeda, T;Ohara, Y;Inokuchi, JI;Nakamura, A; Chronic encephalomyelitis virus exhibits cellular tropism and evades pDCs by binding to sialylated integrins as the cell surface receptors European journal of immunology 2023-08-11 [PMID: 37565654] (Bioassay, Mouse)
• J Vornhagen, B Armistead, V Santana-Uf, C Gendrin, S Merillat, M Coleman, P Quach, E Boldenow, V Alishetti, C Leonhard-M, LY Ngo, C Whidbey, KS Doran, C Curtis, KMA Waldorf, E Nance, L Rajagopal Group B streptococcus exploits vaginal epithelial exfoliation for ascending infection J. Clin. Invest., 2018-04-09;0(0):. 2018-04-09 [PMID: 29629904] (In Vivo, Mouse)
• Tseng C, Kolonin M Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity. Stem Cells, 2015-09-22;34(1):174-90. 2015-09-22 [PMID: 26381424] (Bioassay)

Bioinformatics

• Uniprot:
  • Q3V3R4(Integrin alpha 1) & P09055 (Integrin beta 1)()