Recombinant Mouse IL-5 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-5 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.04-0.15 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-5 protein
Met21-Gly133
Accession #
N-terminal Sequence
Met21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il5
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12-20 kDa, reducing conditions
Publications
Read Publications using
405-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-5 Protein

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Background

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for mouse IL‑5 encodes a signal peptide and a 113 amino acid (aa) mature protein. Mature mouse IL‑5 shares 70%, 94%, 58%, 66%, 59% and 63%, aa sequence identity with human, rat, canine, equine, feline and porcine IL-5, respectively, and shows cross-reactivity with human IL-5 receptor. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

  1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
  3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
  5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
  6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
  7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
  8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
  9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
  10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  11. Tavernier, J. et al. (1991) Cell 66:1175.
  12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
  14. Tavernier, J. et al. (2000) Blood 95:1600.
  15. Kopf, M. et al. (1996) Immunity 4:15.
  16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
  17. Denburg, J. A. et al. (1991) Blood 77:1462.

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405-ML
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Publications for IL-5 (405-ML)(41)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Transgenic Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(31)
Cell Culture
(1)
ELISA (Standard)
(5)
In Vivo
(4)
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Human
(1)
Mouse
(35)
N/A
(4)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using 405-ML Applications Species
A Di Pietro, J Polmear, L Cooper, T Damelang, T Hussain, L Hailes, K O'Donnell, V Udupa, T Mi, S Preston, A Shtewe, U Hershberg, SJ Turner, NL La Gruta, AW Chung, DM Tarlinton, CD Scharer, KL Good-Jacob Targeting BMI-1 in B cells restores effective humoral immune responses and controls chronic viral infection Nature Immunology, 2021;0(0):. 2021 [PMID: 34845392] (Bioassay, Mouse) Bioassay Mouse
M Rogier, J Moritz, I Robert, C Lescale, V Heyer, A Abello, O Martin, K Capitani, M Thomas, AS Thomas-Cla, B Laffleur, F Jouan, E Pinaud, K Tarte, M Cogné, SG Conticello, E Soutoglou, L Deriano, B Reina-San- Fam72a enforces error-prone DNA repair during antibody diversification Nature, 2021;0(0):. 2021 [PMID: 34819671] (Bioassay, Mouse) Bioassay Mouse
K Ochiai, M Yamaoka, A Swaminatha, H Shima, H Hiura, M Matsumoto, D Kurotaki, J Nakabayash, R Funayama, K Nakayama, T Arima, T Ikawa, T Tamura, R Sciammas, P Bouvet, TK Kundu, K Igarashi Chromatin Protein PC4 Orchestrates B Cell Differentiation by Collaborating with IKAROS and IRF4 Cell Reports, 2020;33(12):108517. 2020 [PMID: 33357426] (Bioassay, Mouse) Bioassay Mouse
L Kealy, A Di Pietro, L Hailes, S Scheer, L Dalit, JR Groom, C Zaph, KL Good-Jacob The Histone Methyltransferase DOT1L Is Essential for Humoral Immune Responses Cell Reports, 2020;33(11):108504. 2020 [PMID: 33326791] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
H Gan, T Shen, DP Chupp, JR Taylor, HN Sanchez, X Li, Z Xu, H Zan, P Casali B cell Sirt1 deacetylates histone and non-histone proteins for epigenetic modulation of AID expression and the antibody response Sci Adv, 2020;6(14):eaay2793. 2020 [PMID: 32270032] (Cell Culture, Human) Cell Culture Human
A Calcinotto, A Brevi, M Chesi, R Ferrarese, L Garcia Per, M Grioni, S Kumar, VM Garbitt, ME Sharik, KJ Henderson, G Tonon, M Tomura, Y Miwa, E Esplugues, RA Flavell, S Huber, F Canducci, VS Rajkumar, PL Bergsagel, M Bellone Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression Nat Commun, 2018;9(1):4832. 2018 [PMID: 30510245] (Bioassay, Mouse) Bioassay Mouse
R Willebrand, A Dietschman, L Nitschke, S Krappmann, D Voehringer Murine eosinophil development and allergic lung eosinophilia are largely dependent on the signaling adaptor GRB2 Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30183074] (Bioassay, Mouse) Bioassay Mouse
S Trezise, A Karnowski, PL Fedele, S Mithraprab, Y Liao, K D'Costa, AJ Kueh, MP Hardy, CM Owczarek, MJ Herold, A Spencer, W Shi, SN Willis, SL Nutt, LM Corcoran Mining the Plasma Cell Transcriptome for Novel Cell Surface Proteins Int J Mol Sci, 2018;19(8):. 2018 [PMID: 30042348] (Bioassay, Mouse) Bioassay Mouse
BG Barwick, CD Scharer, RJ Martinez, MJ Price, AN Wein, RR Haines, APR Bally, JE Kohlmeier, JM Boss B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation Nat Commun, 2018;9(1):1900. 2018 [PMID: 29765016] (Bioassay, Mouse) Bioassay Mouse
C Zhu, L Xia, F Li, L Zhou, Q Weng, Z Li, Y Wu, Y Mao, C Zhang, Y Wu, M Li, S Ying, Z Chen, H Shen, W Li mTOR complexes differentially orchestrates eosinophil development in allergy Sci Rep, 2018;8(1):6883. 2018 [PMID: 29720621] (Bioassay, Mouse) Bioassay Mouse
Show All 41 Publications.

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Bioinformatics

Gene Symbol Il5
Uniprot