Recombinant Mouse IL-5 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-5 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.04-0.15 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-5 protein
Met21-Gly133
Accession #
N-terminal Sequence
Met21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il5
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
15-18 kDa, reducing conditions
Publications
Read Publications using
405-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-5 Protein

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Background

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for mouse IL‑5 encodes a signal peptide and a 113 amino acid (aa) mature protein. Mature mouse IL‑5 shares 70%, 94%, 58%, 66%, 59% and 63%, aa sequence identity with human, rat, canine, equine, feline and porcine IL-5, respectively, and shows cross-reactivity with human IL-5 receptor. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

  1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
  3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
  5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
  6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
  7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
  8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
  9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
  10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  11. Tavernier, J. et al. (1991) Cell 66:1175.
  12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
  14. Tavernier, J. et al. (2000) Blood 95:1600.
  15. Kopf, M. et al. (1996) Immunity 4:15.
  16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
  17. Denburg, J. A. et al. (1991) Blood 77:1462.

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Publications for IL-5 (405-ML)(33)

We have publications tested in 2 confirmed species: Mouse, N/A.

We have publications tested in 3 applications: Bioassay, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(24)
ELISA Standard
(5)
In Vivo
(4)
All Applications
Filter By Species
Mouse
(29)
N/A
(4)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using 405-ML Applications Species
BG Barwick, CD Scharer, RJ Martinez, MJ Price, AN Wein, RR Haines, APR Bally, JE Kohlmeier, JM Boss B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation Nat Commun, 2018;9(1):1900. 2018 [PMID: 29765016] (Bioassay, Mouse) Bioassay Mouse
C Zhu, L Xia, F Li, L Zhou, Q Weng, Z Li, Y Wu, Y Mao, C Zhang, Y Wu, M Li, S Ying, Z Chen, H Shen, W Li mTOR complexes differentially orchestrates eosinophil development in allergy Sci Rep, 2018;8(1):6883. 2018 [PMID: 29720621] (Bioassay, Mouse) Bioassay Mouse
CD Scharer, BG Barwick, M Guo, APR Bally, JM Boss Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs Nat Commun, 2018;9(1):1698. 2018 [PMID: 29703886] (Bioassay, Mouse) Bioassay Mouse
Y Kasagi, PM Chandramou, KA Whelan, K Tanaka, V Giroux, M Sharma, J Wang, AJ Benitez, M DeMarshall, JW Tobias, KE Hamilton, GW Falk, JM Spergel, AJ Klein-Szan, AK Rustgi, AB Muir, H Nakagawa The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial�Changes Cell Mol Gastroenterol Hepatol, 2018;5(3):333-352. 2018 [PMID: 29552622] (Bioassay, Mouse) Bioassay Mouse
WR Bolus, KR Peterson, MJ Hubler, AJ Kennedy, ML Gruen, AH Hasty Elevating adipose eosinophils in obese mice to physiologically normal levels does not rescue metabolic impairments Mol Metab, 2017;0(0):. 2017 [PMID: 29306658] (In Vivo, Mouse) In Vivo Mouse
M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista The Small Rho GTPase TC10 Modulates B Cell Immune Responses J. Immunol., 2017;0(0):. 2017 [PMID: 28747344] (Bioassay, Mouse) Bioassay Mouse
J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model PLoS Med., 2017;14(7):e1002365. 2017 [PMID: 28742815] (Bioassay, Mouse) Bioassay Mouse
W Dawicki, C Li, J Town, X Zhang, JR Gordon Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3. 2017 [PMID: 28277274] (ELISA Standard, Mouse) ELISA Standard Mouse
LX Xia, W Hua, Y Jin, BP Tian, ZW Qiu, C Zhang, LQ Che, HB Zhou, YF Wu, HQ Huang, F Lan, YH Ke, JJ Lee, W Li, SM Ying, ZH Chen, HH Shen Eosinophil differentiation in the bone marrow is promoted by protein tyrosine phosphatase SHP2 Cell Death Dis, 2016;7(0):e2175. 2016 [PMID: 27054330] (Bioassay, Mouse) Bioassay Mouse
Schwartz C, Willebrand R, Huber S, Rupec R, Wu D, Locksley R, Voehringer D Eosinophil-specific deletion of IkappaBalpha in mice reveals a critical role of NF-kappaB-induced Bcl-xL for inhibition of apoptosis. Blood, 2015;125(25):3896-904. 2015 [PMID: 25862560] (Bioassay, Mouse) Bioassay Mouse
Show All 33 Publications.

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Bioinformatics

Gene Symbol Il5
Entrez
Uniprot