Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF

Images

 
There are currently no images for IL-4R alpha (530-MR).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.05‑0.2 µg/mL in the presence of 3 ng/mL of recombinant mouse IL-4.
Source
Mouse myeloma cell line, NS0-derived mouse IL-4 R alpha protein
Mouse IL-4 R alpha
(Ile26 - Arg233)
Accession # Q544A9
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus


Accession #
N-terminal Sequence
Ile26
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il4ra
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
65-85 kDa, reducing conditions
Publications
Read Publications using
530-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF

  • CD124 antigen
  • CD124
  • IL-4 R alpha
  • IL-4 receptor subunit alpha
  • IL4R alpha
  • IL-4R alpha
  • IL-4R subunit alpha
  • IL4R
  • IL-4Ra
  • IL4RACD124
  • IL-4R-alpha
  • interleukin 4 receptor
  • interleukin-4 receptor alpha chain
  • interleukin-4 receptor subunit alpha

Background

Interleukin 4 Receptor alpha (IL-4 R alpha ), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1 ‑ 5). Mature mouse IL-4 R alpha consists of a 208 amino acid (aa) extracellular domain (ECD) that contains a ligand binding region and one fibronectin type-III domain, a 24 aa transmembrane segment, and a 553 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6). Within the ECD, mouse IL-4 R alpha shares 51% and 76% aa sequence identity with human and rat IL-4 R alpha, respectively. Alternate splicing of mouse IL-4 R alpha generates a secreted isoform and an isoform that lacks the cytoplasmic region (6 - 8). Proteolytic cleavage can also release soluble IL-4 R alpha, and both these mechanisms produce a molecule which retains ligand binding properties and inhibits IL-4 bioactivity (6, 7, 9). IL-4 R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (10). It can associate with the common gamma chain ( gamma c) to form the IL-4 responsive type I receptor in which gamma c increases the affinity for IL-4 and enables signaling (11, 12). It can alternatively associate with IL-13 R alpha 1 to form the type II receptor which is responsive to both IL-4 and IL-13 (13, 14). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize gamma c (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (15, 16).
  1. Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55.
  2. Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
  3. Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
  4. Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
  5. Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
  6. Mosley, B. et al. (1989) Cell 59:335.
  7. Blum, H. et al. (1996) J. Immunol. 157:1846.
  8. Wrighton, N. et al. (1992) Growth Factors 6:103.
  9. Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
  10. Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
  11. Kondo, M. et al. (1993) Science 262:1874.
  12. Russell, S.M. et al. (1993) Science 262:1880.
  13. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
  14. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  15. Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
  16. Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.

Customers Who Viewed This Item Also Viewed...

NBP2-25241
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NB7343
Species: Ca
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB100-64358
Species: Hu
Applications: Flow, IHC, IHC-P
AF152
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF146
Species: Hu
Applications: WB, Simple Western, Flow, IHC, Block, CyTOF-ready
NBP1-88968
Species: Hu
Applications: WB, IHC, IHC-P

Publications for IL-4R alpha (530-MR)(2)

Reviews for IL-4R alpha (530-MR) (0)

There are no reviews for IL-4R alpha (530-MR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-4R alpha (530-MR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse IL-4R alpha Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Il4ra
Uniprot