Recombinant Mouse IL-34 His-tag Protein, CF

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 10.0-100 ng/mL.

Equivalent Bioactivity of Recombinant Mouse IL‑34 His-tag Protein (Catalog # 11653-ML) and Recombinant Mouse IL-34 Protein (5195-ML) as measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells (orange and green, respectively).

2 μg/lane of Recombinant Mouse IL‑34 His-tag Protein (Catalog # 11653-ML) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 21-32 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-34 His-tag Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED₅₀ for this effect is 10.0‑100 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse IL-34 protein
  Asn21-Leu193, with a C-terminal 6-His tag
• Accession #: Q8R1R4.1
• N-terminal Sequence: Asn21
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 21-32 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-34 His-tag Protein, CF

• C16orf77
• IL34
• IL-34
• interleukin 34

Background

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77 homolog) is a 39 kDa, secreted cytokine that belongs to no known cytokine family (1). Mouse IL-34 is synthesized as a 235 amino acid (aa) precursor with a 20 aa signal sequence and a 215 aa mature chain (SwissProt # Q8R1R4). The mature chain contains two potential sites for N-linked glycosylation. There are three isoforms for IL-34. Isoform 1 (Q8R1R4-1) is the recombinant mouse IL-34 described in this insert. Isoform 2 (Q8R1R4-2) has an 85 aa substitution for the final 101 aa’s in isoform 1, and isoform 3 (Q8R1R4-3) lacks Q81 in isoform 1. Mouse IL-34 is 71% identical to human IL-34 on the amino acid level. IL-34 is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and it is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte viability (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

1. Lin, H. et al. (2008) Science 320:807.

Bioinformatics

• Uniprot:
  • Q8R1R4.1()