Recombinant Mouse IL-21 Protein, CF


1 μg/lane of Recombinant Mouse IL-21 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 15 kDa.
Recombinant Mouse IL-21 (Catalog # 594‑ML/CF) enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 is ≤8 ng/mL.

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-21 Protein, CF Summary

Details of Functionality
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 3-30 ng/mL.
E. coli-derived mouse IL-21 protein
Pro25-Ser146, with and without an N-terminal Met
Accession #
N-terminal Sequence
Pro25 & Met
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
14.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
594-ML/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-21 Protein, CF

  • CVID11
  • IL21
  • IL-21
  • IL-21Za11interleukin-21
  • interleukin 21
  • interleukin-21 isoform
  • Za11


Interleukin-21 (IL-21) is an approximately 14 kDa four-helix-bundle cytokine in the family of cytokines that utilize the common gamma chain ( gamma c) as a receptor subunit.  gamma c is also a subunit of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (1). IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells (2-6). It exerts its biological effects through a heterodimeric receptor complex of gamma c and the IL-21-specific IL-21 R (2, 7). Tfh-derived IL-21 plays an important role in the development of humoral immunity through its autocrine effects on the Tfh cell and paracrine effects on immunoglobulin affinity maturation, plasma cell differentiation, and B cell memory responses (4, 8, 9). It is also required for the migration of dendritic cells to draining lymph nodes (10). IL-21 regulates several aspects of T cell function. It co‑stimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization (3, 5, 6, 11, 12). It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells (13, 14). IL-21 R engagement enhances the cytolytic activity and IFN-gamma production of activated NK cells but limits the expansion of resting NK cells (15). In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation (16). Dysregulation of the IL‑21/IL‑21 R system contributes to the development of multiple immunological disorders (1, 17). The mouse IL‑21 precursor contains a predicted 17 amino acid (aa) signal sequence and a 129 aa mature chain. Mature mouse IL-21 shares 66%, 59%, 58%, and 88% aa sequence identity with mature canine, human, rabbit, and rat IL-21, respectively.

  1. Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348.
  2. Parrish-Novak, et al. (2000) Nature 408:57.
  3. Coquet, J.M. et al. (2007) J. Immunol. 178:2827.
  4. Vogelzang, A. et al. (2008) Immunity 29:127.
  5. Korn, T. et al. (2007) Nature 448:484.
  6. Nurieva, R. et al. (2007) Nature 448:480.
  7. Asao, H. et al. (2001) J. Immunol. 167:1.
  8. Zotos, D. et al. (2010) J. Exp. Med. 207:365.
  9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  10. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  11. Frohlich, A. et al. (2009) Science 324:1576.
  12. Yi, J.S., et al. (2009) Science 324:1572.
  13. Peluso, I. et al. (2007) J. Immunol. 178:732.
  14. Bucher, C. et al. (2009) Blood 114:5375.
  15. Kasaian, M.T. et al. (2002) Immunity 16:559.
  16. Tamagawa-Mineoka, R. et al. (2011) J. Invest. Dermatol. 131:1513.
  17. Ma, J. et al. (2011) Cytokine 56:133.

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Publications for IL-21 (594-ML/CF)(40)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 5 applications: Bioassay, Cell Culture, Flow Cytometry, In Vivo, Western Blot.

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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using 594-ML/CF Applications Species
D Zotos, I Quast, CSN Li-Wai-Sue, CI McKenzie, MJ Robinson, A Kan, GK Smyth, PD Hodgkin, DM Tarlinton The concerted change in the distribution of cell cycle phases and zone composition in germinal centers is regulated by IL-21 Nature Communications, 2021;12(1):7160. 2021 [PMID: 34887406] (Bioassay, Mouse) Bioassay Mouse
A Singh, M Dashynam, B Chim, TM Escobar, X Liu, X Hu, S Patnaik, X Xu, N Southall, J Marugan, A Jadhav, V Lazarevic, SA Muljo, M Ferrer Identification of Small Molecule Inhibitors of a Mir155 Transcriptional Reporter in Th17 Cells Scientific Reports, 2021;11(1):11498. 2021 [PMID: 34075120] (Bioassay, Mouse) Bioassay Mouse
M Matsuda, K Doi, T Tsutsumi, M Inaba, J Hamaguchi, T Terada, R Kawata, K Kitatani, T Nabe Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice J. Pharmacol. Sci., 2019;141(4):139-145. 2019 [PMID: 31744690] (Cell Culture, Mouse) Cell Culture Mouse
X Yang, J Zhao, S Duan, X Hou, X Li, Z Hu, Z Tang, F Mo, X Lu Enhanced cytotoxic T lymphocytes recruitment targeting tumor vasculatures by endoglin aptamer and IP-10 plasmid presenting liposome-based nanocarriers Theranostics, 2019;9(14):4066-4083. 2019 [PMID: 31281532] (Bioassay, Mouse) Bioassay Mouse
WF Yen, R Sharma, M Cols, CM Lau, A Chaudhry, P Chowdhury, WT Yewdell, B Vaidyanath, A Sun, M Coffre, JN Pucella, CC Chen, M Jasin, JC Sun, AY Rudensky, SB Koralov, J Chaudhuri Distinct Requirements of CHD4 during B Cell Development and Antibody Response Cell Rep, 2019;27(5):1472-1486.e5. 2019 [PMID: 31042474] (Bioassay, Mouse) Bioassay Mouse
M Amir, S Chaudhari, R Wang, S Campbell, SA Mosure, LB Chopp, Q Lu, J Shang, OB Pelletier, Y He, C Doebelin, MD Cameron, DJ Kojetin, TM Kamenecka, LA Solt REV-ERB? Regulates TH17 Cell Development and Autoimmunity Cell Rep, 2018;25(13):3733-3749.e8. 2018 [PMID: 30590045]
Q Cheng, J Liu, Y Pei, Y Zhang, D Zhou, W Pan, J Zhang Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection PLoS Pathog., 2018;14(11):e1007440. 2018 [PMID: 30462731] (Bioassay, Mouse) Bioassay Mouse
B Zwarycz, AD Gracz, KR Rivera, IA Williamson, LA Samsa, J Starmer, MA Daniele, L Salter-Cid, Q Zhao, ST Magness IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model Cell Mol Gastroenterol Hepatol, 2019;7(1):1-17. 2019 [PMID: 30364840] (Bioassay, Mouse) Bioassay Mouse
W Ise, K Fujii, K Shiroguchi, A Ito, K Kometani, K Takeda, E Kawakami, K Yamashita, K Suzuki, T Okada, T Kurosaki T Follicular Helper Cell-Germinal Center B Cell Interaction Strength Regulates Entry into Plasma Cell or Recycling Germinal Center Cell Fate Immunity, 2018;48(4):702-715.e4. 2018 [PMID: 29669250] (Bioassay, Mouse) Bioassay Mouse
B Sun, L Zhu, Y Tao, HX Sun, Y Li, P Wang, Y Hou, Y Zhao, X Zhang, L Zhang, N Na, Y Zhao Characterization and allergic role of IL-33-induced neutrophil polarization Cell. Mol. Immunol., 2018;0(0):. 2018 [PMID: 29503441] (Bioassay, Mouse) Bioassay Mouse
Show All 40 Publications.

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Gene Symbol Il21