Recombinant Mouse IL-17E (NS0-expressed) Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-17E (NS0-expressed) Protein, CF Summary

Details of Functionality
Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.2-1.0 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-17E/IL-25 protein
Val17-Ala169
Accession #
N-terminal Sequence
Val17
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il25
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-27 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-17E (NS0-expressed) Protein, CF

  • IL17E
  • IL-17E
  • IL25
  • IL-25
  • interleukin 25
  • Interleukin-17E
  • interleukin-25

Background

The Interleukin‑17 (IL‑17) family of proteins are immunoregulatory cytokines that share a conserved cysteine‑rich region. IL‑17E, which is also known as IL‑25, promotes Th2‑biased immune responses. This is in contrast to other IL‑17 family members which promote Th1‑ and Th17‑biased inflammation. IL‑25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature mouse IL‑25 shares 80% and 91% amino acid sequence identity with human and rat IL‑25, respectively (3, 4). During helminth infections and allergic reactions, IL‑25 is locally upregulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4‑9). It binds to IL‑17 RB but also requires IL‑17 RA to exert its activity (3, 8, 10). IL‑25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL‑4, IL‑5, IL‑13) and reduced production of Th1 and Th17 cytokines (e.g. IFN‑ gamma, IL‑12, IL‑23, IL‑17A, IL‑17F) (4‑6, 8, 9, 11‑15). Airway IL‑25 can be activated by MMP‑7, a protease that is up‑regulated in airway epithelium in response to allergen exposure (16). Cleaved IL‑25 shows enhanced binding to IL‑17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL‑25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis (12, 15). IL‑25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood‑brain barrier and limits CD4+ T cell infiltration into the brain (17).
  1. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
  2. Iwakura, Y. et al. (2011) Immunity 34:149.
  3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  4. Fort, M.M. et al. (2001) Immunity 15:985.
  5. Zhao, A. et al. (2010) J. Immunol. 185:6921.
  6. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
  7. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
  8. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
  9. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
  10. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
  11. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  12. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
  13. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
  14. Stock, P. et al. (2009) J. Immunol. 182:5116.
  15. Caruso, R. et al. (2009) Gastroenterology 136:2270.
  16. Goswami, S. et al. (2009) Nat. Immunol. 10:496.
  17. Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.

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Bioinformatics

Gene Symbol Il25
Uniprot