Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmGITR/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmGITR Ligand with a linear range of 0.8-50 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse GITR/TNFRSF18 protein
  

  Mouse GITR (Ser22-His153) Accession # Q8C4K3	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: Q8C4K3
• N-terminal Sequence: Ser22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfrsf18
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 42 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF

• Activation-inducible TNFR family receptor
• AITR
• AITRTNF receptor superfamily activation-inducible protein
• CD357 antigen
• CD357
• GITR
• GITR-D
• GITRtumor necrosis factor receptor superfamily member 18
• Glucocorticoid-induced TNFR-related protein
• TNFRSF18
• tumor necrosis factor receptor superfamily, member 18

Background

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature mouse GITR consists of a 134 amino acid (aa) extracellular domain (ECD) with three tandem TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (3). Within the ECD, mouse GITR shares 55% and 86% aa sequence identity with human and rat GITR, respectively. Alternative splicing generates additional isoforms with substituted cytoplasmic domains or with a substitution within the third TNFR repeat, transmembrane, and cytoplasmic regions (4). GITR is expressed on CD4⁺CD25⁺ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (3, 5, 6), and it is upregulated on antigen-activated conventional CD4⁺ and CD8⁺ T cells (3, 6-8). GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4⁺ or CD8⁺ conventional T cells (7-10). It also induces the proliferation of Treg (9, 11) but inhibits the ability of Treg to suppress immune responses (5, 9, 12-14). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (5, 12), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (11, 15). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (16).

1. Clouthier, D.L. and T.H. Watts (2014) Cytokine Growth Factor Rev. 25:91.
2. Ronchetti, S. et al. (2015) J. Immunol. Res. 2015:171520.
3. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
4. Nocentini, G. et al. (2000) Cell Death Differ. 7:408.
5. Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
6. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
7. Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
8. Muriglan, S.J. et al. (2004) J. Exp. Med. 200:149.
9. Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
10. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
11. Ephrem, A. et al. (2013) Eur. J. Immunol. 43:2421.
12. Ko, K. et al. (2005) J. Exp. Med. 202:885.
13. Ji, H. et al. (2004) J. Immunol. 172:5823.
14. Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
15. Patel, M. et al. (2005) Eur. J. Immunol. 35:3581.
16. O'Keeffe, G.W. et al. (2008) Nat. Neurosci. 11:135.

Reviews for GITR/TNFRSF18 (524-GR) (2)

Average Rating: 4.5

(Based on 2 reviews)

reviewed by: Verified Customer Binding assay/Protein-protein interaction 06/09/2019

Summary

• Application: Binding assay/Protein-protein interaction
• Lot: BEV0416041

reviewed by: Verified Customer Binding assay/Protein-protein interaction 11/16/2018

Summary

• Application: Binding assay/Protein-protein interaction
• Lot: BEV0416041

Comments

• Comments: Measured by functional ELISA. Recombinant mouse GITR/TNFRSF18 at 2 µg/ml binds mouse TNFSF18 in a dose dependent manner.

Bioinformatics

• Gene Symbol: Tnfrsf18
• Uniprot:
  • Q8C4K3()