Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmGITR/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmGITR Ligand with a linear range of 0.8-50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse GITR/TNFRSF18 protein
Mouse GITR
(Ser22-His153)
Accession # Q8C4K3
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Tnfrsf18
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
42 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55-65 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
using
524-GR in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF

  • Activation-inducible TNFR family receptor
  • AITR
  • AITRTNF receptor superfamily activation-inducible protein
  • CD357 antigen
  • CD357
  • GITR
  • GITR-D
  • GITRtumor necrosis factor receptor superfamily member 18
  • Glucocorticoid-induced TNFR-related protein
  • TNFRSF18
  • tumor necrosis factor receptor superfamily, member 18

Background

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature mouse GITR consists of a 134 amino acid (aa) extracellular domain (ECD) with three tandem TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (3). Within the ECD, mouse GITR shares 55% and 86% aa sequence identity with human and rat GITR, respectively. Alternative splicing generates additional isoforms with substituted cytoplasmic domains or with a substitution within the third TNFR repeat, transmembrane, and cytoplasmic regions (4). GITR is expressed on CD4+CD25+ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (3, 5, 6), and it is upregulated on antigen-activated conventional CD4+ and CD8+ T cells (3, 6-8). GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4+ or CD8+ conventional T cells (7-10). It also induces the proliferation of Treg (9, 11) but inhibits the ability of Treg to suppress immune responses (5, 9, 12-14). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (5, 12), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (11, 15). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (16).
  1. Clouthier, D.L. and T.H. Watts (2014) Cytokine Growth Factor Rev. 25:91.
  2. Ronchetti, S. et al. (2015) J. Immunol. Res. 2015:171520.
  3. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  4. Nocentini, G. et al. (2000) Cell Death Differ. 7:408.
  5. Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
  6. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  7. Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
  8. Muriglan, S.J. et al. (2004) J. Exp. Med. 200:149.
  9. Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
  10. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
  11. Ephrem, A. et al. (2013) Eur. J. Immunol. 43:2421.
  12. Ko, K. et al. (2005) J. Exp. Med. 202:885.
  13. Ji, H. et al. (2004) J. Immunol. 172:5823.
  14. Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
  15. Patel, M. et al. (2005) Eur. J. Immunol. 35:3581.
  16. O'Keeffe, G.W. et al. (2008) Nat. Neurosci. 11:135.

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Review for GITR/TNFRSF18 (524-GR) (1) 51

Average Rating: 5
(Based on 1 review)

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reviewed by:
Anonymous
11/16/2018
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Summary

LotBEV0416041

Comments

CommentsMeasured by functional ELISA. Recombinant mouse GITR/TNFRSF18 at 2 µg/ml binds mouse TNFSF18 in a dose dependent manner.

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Anonymous
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Bioinformatics

Gene Symbol Tnfrsf18
Uniprot