Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Artemin (Catalog # 1085-AR) at 1 µg/mL can bind Recombinant Mouse GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera with an apparent K_d <1 nM.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse GFR alpha-3/GDNF R alpha-3 protein
  

  Met	Mouse GFR alpha -3 (Glu34-Arg379) Accession # AAB70931	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus				C-terminus

• Accession #: AAB70931
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Gfra3
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 66.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF

• GDNF family receptor alpha 3
• GDNF family receptor alpha-3
• GDNF R alpha-3
• GDNF receptor alpha-3
• GDNFR-alpha-3
• GFR alpha3
• GFR alpha-3
• GFRA3
• GFRa-3
• GFR-alpha-3
• glial cell line-derived neurotrophic factor receptor alpha-3
• GPI-linked receptor

Background

Mouse glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha -3, GDNF R alpha -3) is an approximately 50 kDa plasma membrane glycoprotein that is one of four GDNF receptors, all of which are glycosylphosphoinositol (GPI)-anchored, contain three conserved cysteine repeats, and promote survival of neurons (1‑4). It is synthesized as a 397 aa precursor with a 28 aa N‑terminal signal sequence, a 343 aa mature segment, and a C-terminal GPI attachment signal sequence (3‑5). Mouse GFR alpha -3 shares 94% aa sequence identity with rat GFR alpha -3 and 76‑81% aa sequence identity with human, equine, bovine and canine GFR alpha -3. GFR alpha -3 is expressed in the central nervous system only during the earliest stages of neurogenesis, while later it is predominantly found in developing and adult nociceptive sensory neurons (2, 4‑8). It is expressed with the shared GDNF co‑receptor, the Ret receptor tyrosine kinase, in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (2, 4). Its ligand, the GDNF family ligand artemin, is primarily expressed by immature Schwann cells in the peripheral nervous system, and vascular smooth muscle cells, directing axonal projection of sympathetic neurons (6, 7). Artemin first binds GFR alpha -3, which recruits Ret, forming a signaling complex that is a pentamer containing one artemin, two GFR alpha -3, and two Ret molecules (1, 6, 9). Signals from this complex are required for the development and survival of the superior cervical ganglion (SCG) neurons, and deletion of mouse GFR alpha -3 results in deficits in the SCG and inhibited development of intestinal Peyer’s patches (6, 7, 10, 11).

1. Wang, X. et al. (2006) Structure 14:1083.
2. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
3. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
4. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
5. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
6. Baloh, R. et al. (1998) Neuron 21:1291.
7. Honma, Y. et al. (2002) Neuron 35:267.
8. Orozco, O.E. et al.(2001) Eur. J. Neurosci. 13:2177.
9. Schlee, S. et al. (2006) Nat. Chem. Biol. 2:636.
10. Nishino, J et al. (1999) Neuron 23:725.
11. Veiga-Fernandez, H. et al. (2007) Nature 446:547.

Publications for GFR alpha-3/GDNF R alpha-3 (2645-FR)(1)

Publication using 2645-FR

• Li J, Klein C, Liang C, Rauch R, Kawamura K, Hsueh AJ Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice. FEBS Lett., 2009-07-04;583(15):2479-85. 2009-07-04 [PMID: 19580811] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Gfra3
• Uniprot:
  • AAB70931()