Recombinant Mouse Gas6 Protein

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Summary
Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse Gas6 Protein Summary

Details of Functionality
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 20-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Gas6 protein
Asp115-Pro673, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asp115
Protein/Peptide Type
Recombinant Proteins
Gene
Gas6
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
72 kDa, reducing conditions
Publications
Read Publications using
986-GS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Gas6 Protein

  • AXLLG
  • AXLLGAXL stimulatory factor
  • AXSFAXL receptor tyrosine kinase ligand
  • DKFZp666G247
  • FLJ34709
  • Gas6
  • GAS-6
  • growth arrest-specific 6
  • growth arrest-specific protein 6

Background

Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium.

Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.

  1. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  2. Goruppi, S. et al. (1996) Oncogene 12:471.
  3. Crosier, K and P. Crosier (1997) Pathology 29:131.
  4. Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
  5. Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.

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Publications for Gas6 (986-GS)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(15)
Cell Culture
(4)
ELISA (Standard)
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(16)
Rat
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 986-GS Applications Species
T Shibata, A Makino, R Ogata, S Nakamura, T Ito, K Nagata, Y Terauchi, T Oishi, M Fujieda, Y Takahashi, M Ato Respiratory syncytial virus infection exacerbates pneumococcal pneumonia via Gas6/Axl-mediated macrophage polarization J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32364537] (In Vivo, Mouse) In Vivo Mouse
A Kurata, Y Tachibana, T Takahashi, N Horiba Novel AXL-specific inhibitor ameliorates kidney dysfunction through the inhibition of epithelial-to-mesenchymal transition of renal tubular cells PLoS ONE, 2020;15(4):e0232055. 2020 [PMID: 32324796] (Cell Culture, Rat) Cell Culture Rat
A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31689560] (Cell Culture, Mouse) Cell Culture Mouse
LM Chirino, S Kumar, M Okumura, DE Sterner, M Mattern, TR Butt, T Kambayashi TAM receptors attenuate murine NK-cell responses via E3 ubiquitin ligase Cbl-b Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 31531847] (Cell Culture, Mouse) Cell Culture Mouse
CK Peng, CP Wu, JY Lin, SC Peng, CH Lee, KL Huang, CH Shen Gas6/Axl signaling attenuates alveolar inflammation in ischemia-reperfusion-induced acute lung injury by up-regulating SOCS3-mediated pathway PLoS ONE, 2019;14(7):e0219788. 2019 [PMID: 31318922] (Bioassay, Mouse) Bioassay Mouse
CK Peng, CP Wu, JY Lin, SC Peng, CH Lee, KL Huang, CH Shen Gas6/Axl signaling attenuates alveolar inflammation in ischemia-reperfusion-induced acute lung injury by up-regulating SOCS3-mediated pathway PLoS ONE, 2019;14(7):e0219788. 2019 [PMID: 31318922] (Cell Culture, Mouse) Cell Culture Mouse
J Ni, M Lin, Y Jin, J Li, Y Guo, J Zhou, G Hong, G Zhao, Z Lu Gas6 Attenuates Sepsis-Induced Tight Junction Injury and Vascular Endothelial Hyperpermeability via the Axl/NF-kappaB Signaling Pathway Front Pharmacol, 2019;10(0):662. 2019 [PMID: 31263416] (Bioassay, Human) Bioassay Human
J Jung, YJ Lee, YH Choi, EM Park, HS Kim, JL Kang Gas6 Prevents Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells via Production of PGE2, PGD2 and Their Receptors Cells, 2019;8(7):. 2019 [PMID: 31247991] (Bioassay, Mouse) Bioassay Mouse
Y Jiang, Y Zhang, JY Leung, C Fan, KI Popov, S Su, J Qian, X Wang, A Holtzhause, E Ubil, Y Xiang, I Davis, NV Dokholyan, G Wu, CM Perou, WY Kim, HS Earp, P Liu MERTK mediated novel site Akt phosphorylation alleviates SAV1 suppression Nat Commun, 2019;10(1):1515. 2019 [PMID: 30944303] (Bioassay, Human) Bioassay Human
AM Gomes, EC Carron, KL Mills, AM Dow, Z Gray, CR Fecca, MA Lakey, P Carmeliet, F Kittrell, D Medina, HL Machado Stromal Gas6 promotes the progression of premalignant mammary cells Oncogene, 2018;0(0):. 2018 [PMID: 30531835] (Bioassay, Mouse) Bioassay Mouse
Show All 22 Publications.

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Bioinformatics

Gene Symbol Gas6
Uniprot