Recombinant Mouse Gas6 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Mouse Gas6 Protein, CF Summary

Details of Functionality
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 20-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Gas6 protein
Asp115-Pro673, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asp115
Protein/Peptide Type
Recombinant Proteins
Gene
Gas6
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
72 kDa, reducing conditions
Publications
Read Publications using
986-GS/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Gas6 Protein, CF

  • AXLLG
  • AXLLGAXL stimulatory factor
  • AXSFAXL receptor tyrosine kinase ligand
  • DKFZp666G247
  • FLJ34709
  • Gas6
  • GAS-6
  • growth arrest-specific 6
  • growth arrest-specific protein 6

Background

Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homologue. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium.

Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.

  1. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  2. Goruppi, S. et al. (1996) Oncogene 12:471.
  3. Crosier, K and P. Crosier (1997) Pathology 29:131.
  4. Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
  5. Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.

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Publications for Gas6 (986-GS/CF)(11)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 3 applications: Binding Assay, Bioassay, ELISA Standard.


Filter By Application
Binding Assay
(1)
Bioassay
(9)
ELISA Standard
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(8)
N/A
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 986-GS/CF Applications Species
A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain Neuron, 2017;94(3):500-516.e9. 2017 [PMID: 28472653] (Bioassay, Mouse) Bioassay Mouse
Lee Y, Park H, Woo S, Park E, Kang J RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages. J Pharmacol Exp Ther, 2014;350(3):563-77. 2014 [PMID: 24939420] (Bioassay, Mouse) Bioassay Mouse
Ji R, Tian S, Lu H, Lu Q, Zheng Y, Wang X, Ding J, Li Q, Lu Q TAM receptors affect adult brain neurogenesis by negative regulation of microglial cell activation. J Immunol, 2013;191(12):6165-77. 2013 [PMID: 24244024] (Bioassay, Mouse) Bioassay Mouse
Deng T, Zhang Y, Chen Q, Yan K, Han D Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages. Immunology, 2012;135(1):40-50. 2012 [PMID: 22043818] (Bioassay, Mouse) Bioassay Mouse
Ye X, Li Y, Stawicki S An anti-Axl monoclonal antibody attenuates xenograft tumor growth and enhances the effect of multiple anticancer therapies. Oncogene, 2010;29(38):5254-64. 2010 [PMID: 20603615] (Binding Assay, Mouse) Binding Assay Mouse
Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway. J. Immunol., 2009;183(5):3004-13. 2009 [PMID: 19657094] (Bioassay, Human) Bioassay Human
Lafdil F, Chobert MN, Deveaux V, Zafrani ES, Mavier P, Nakano T, Laperche Y, Brouillet A Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice. J. Hepatol., 2009;51(1):55-66. 2009 [PMID: 19443073] (Bioassay, Mouse) Bioassay Mouse
Uehara H, Shacter E Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells. J. Immunol., 2008;180(4):2522-30. 2008 [PMID: 18250462] (Bioassay, Human) Bioassay Human
Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P Role of Gas6 in erythropoiesis and anemia in mice. J. Clin. Invest., 2008;118(2):583-96. 2008 [PMID: 18188450] (ELISA Standard, N/A) ELISA Standard N/A
Rothlin CV, Ghosh S, Zuniga EI, Oldstone MB, Lemke G TAM receptors are pleiotropic inhibitors of the innate immune response. Cell, 2007;131(6):1124-36. 2007 [PMID: 18083102] (Bioassay, Mouse) Bioassay Mouse
Show All 11 Publications.

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Bioinformatics

Gene Symbol Gas6
Entrez
Uniprot