Recombinant Mouse Flt-3/Flk-2 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Flt-3/Flk-2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro‑B cells transfected with Flt-3. The ED₅₀ for this effect is 0.05-0.25 µg/mL in the presence of recombinant mouse Flt-3 Ligand at 10 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Flt-3/Flk-2 protein
  

  Mouse Flt-3 (Asn28-Ser544) Accession # Q00342	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: Q00342
• N-terminal Sequence: Asn28
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Flt3
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 85 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 116 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Flt-3/Flk-2 Fc Chimera Protein, CF

• CD135 antigen
• CD135
• EC 2.7.10
• fetal liver kinase 2
• FL cytokine receptor
• FLK2
• Flk-2
• FLT3 receptor tyrosine kinase
• Flt3
• Flt-3
• Fms-like tyrosine kinase 3
• fms-related tyrosine kinase 3
• growth factor receptor tyrosine kinase type III
• Stem cell tyrosine kinase 1
• STK-1
• STK1EC 2.7.10.1
• Tyrosine-protein kinase receptor FLT3

Background

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1 (stem cell tyrosine kinase), is a member of the class III subfamily of receptor tyrosine kinases. This familay includes KIT, the receptor for SCF, and C-FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Mouse Flt-3 cDNA encodes a 992 amino acid (aa) residue type I membrane protein with a 27 aa residue signal peptide, a 517 aa extracellular domain with 10 potential N-linked glycosylation sites, a 20 aa residue transmembrane domain and a 428 aa residue cytoplasmic domain. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

1. Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
2. Drexler, H.G. (1996) Leukemia 10:588.

Publications for Flt-3/Flk-2/CD135 (768-F3)(4)

Publications using 768-F3

• A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain Progress in neurobiology, 2023-01-13;0(0):102405. 2023-01-13 [PMID: 36646299] (Bioassay, SPR, Mouse)
• Sakabe I, Asai A, Iijima J, Maruyama M Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells. Immun Ageing, 2012-04-11;9(0):2. 2012-04-11 [PMID: 22494997] (Differentiation, Differentiation, Mouse)
• Yuan W, Payton JE, Holt MS, Link DC, Watson MA, DiPersio JF, Ley TJ Commonly dysregulated genes in murine APL cells. Blood, 2006-09-28;109(3):961-70. 2006-09-28 [PMID: 17008535] (In Vivo, Mouse)
• Yilmaz OH, Kiel MJ, Morrison SJ SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity. Blood, 2005-10-11;107(3):924-30. 2005-10-11 [PMID: 16219798] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Flt3
• Uniprot:
  • Q00342()