Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF Summary

• Details of Functionality: Measured by its ability to bind mouse IgG with an estimated K_D < 150 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Fc gamma RIIB/CD32b protein
  Thr30-Arg207, with a C-terminal 10-His tag
• Accession #: P08101
• N-terminal Sequence: Thr30
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fcgr2b
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF

• CD32 antigen
• CD32b
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RIIB
• FCG2
• Fc-gamma RII-b
• fc-gamma-RIIb
• FCGR2
• FCGR2B
• FcgRIIB
• FCRIIB
• fcRII-b
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1 - 3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10^-8 - 10^-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10^-6 - 10^-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group. These proteins share 94 - 99% amino acid identity in their extracellular domains but have divergent cytoplasmic domains and signaling capacities (1, 3). In contrast, a single gene encodes mouse CD32 (4). The protein product delivers an inhibitory signal upon ligand binding and is functionally equivalent to human Fc gamma RIIB (5). Mouse CD32 is the only Fc gamma R expressed on B cells and is also expressed on macrophages, neutrophils and mast cells. Mouse CD16 is closely related to mouse CD32 throughout its extracellular domain (95% amino acid identity), but has a divergent cytoplasmic domain and functions as an activating receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Publications for Fc gamma RIIB/CD32b (1460-CD)(5)

Publications using 1460-CD

• Taghipour-Mirakmahaleh, R;Morin, F;Zhang, Y;Bourhoven, L;Béland, LC;Zhou, Q;Jaworski, J;Park, A;Dominguez, JM;Corbeil, J;Flanagan, EP;Marignier, R;Larochelle, C;Kerfoot, S;Vallières, L; Turncoat antibodies unmasked in a model of autoimmune demyelination: from biology to therapy bioRxiv : the preprint server for biology 2024-12-06 [PMID: 39677612] (Surface Plasmon Resonance (SPR), N/A)
• RF Iyer, DM Edwards, P Kolb, HP Raué, CA Nelson, ML Epperson, MK Slifka, JC Nolz, H Hengel, DH Fremont, K Früh The secreted protein Cowpox Virus 14 contributes to viral virulence and immune evasion by engaging Fc-gamma-receptors PloS Pathogens, 2022-09-19;18(9):e1010783. 2022-09-19 [PMID: 36121874] (Bioassay, Mouse)
• E Dodagatta-, HY Ma, B Liang, J Li, DS Meyer, SY Chen, KH Sun, X Ren, B Zivak, MD Rosenblum, MB Headley, L Pinzas, NI Reed, JS Del Cid, BC Hann, S Yang, A Giddabasap, K Noorbehesh, B Yang, J Dal Porto, T Tsukui, K Niessen, A Atakilit, RJ Akhurst, D Sheppard Integrin &amp;alphav&amp;beta8 on T�cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy Cell Reports, 2021-07-06;36(1):109309. 2021-07-06 [PMID: 34233193] (Bioassay, Mouse)
• A Iskenderia, N Liu, Q Deng, Y Huang, C Shen, K Palmieri, R Crooker, D Lundberg, N Kastrapeli, B Pescatore, A Romashko, J Dumas, R Comeau, A Norton, J Pan, H Rong, K Derakhchan, DE Ehmann Myostatin and activin blockade by engineered follistatin results in hypertrophy and improves dystrophic pathology in mdx mouse more than myostatin blockade alone Skelet Muscle, 2018-10-27;8(1):34. 2018-10-27 [PMID: 30368252] (In Vivo, Mouse)
• Shanmugarajan S, Beeson CC, Reddy SV Osteoclast inhibitory peptide-1 binding to the Fc gammaRIIB inhibits osteoclast differentiation. Endocrinology, 2010-07-07;151(9):4389-99. 2010-07-07 [PMID: 20610564] (Surface Plasmon Resonance, Mouse)

Bioinformatics

• Gene Symbol: Fcgr2b
• Uniprot:
  • P08101()