Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF

Images

 
There are currently no images for Ephrin-B2 (496-EB).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to compete with Biotinylated Recombinant Mouse Ephrin‑B2 Fc Chimera (Catalog # BT496) for binding with immobilized Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2) in a functional ELISA assay.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-B2 protein
Mouse Ephrin-B2
(Arg27-Ala227)
Accession # AAA82934
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Efnb2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
49.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-65 kDa, reducing conditions
Publications
Read Publications using
496-EB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF

  • EFNB2
  • ELF-2
  • ephrin B2
  • EphrinB2
  • Ephrin-B2
  • EPLG5
  • HTKL
  • Htk-L
  • LERK5
  • LERK-5
  • NLERK-1

Background

Ephrin‑B2, also known as Htk‑L, ELF‑2, LERK‑5, and NLERK‑1, is a 40 kDa member of the Ephrin‑B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin‑B ligands are structurally related to GPI‑anchored Ephrin‑A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑B2 preferentially interacts with receptors in the EphB family (1, 2). Mature mouse Ephrin‑B2 consists of a 204 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain [Cerretti 1197, Bergemann 4921, Bennett 1866]. Within the ECD, mouse Ephrin‑B2 shares 97% and 98% aa sequence identity with human and rat Ephrin‑B2, respectively. Ephrin‑B2 is expressed presynaptically on neurons (6, 7). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (6‑8). Ephrin‑B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (9, 10). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (8‑11). Ephrin‑B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (12, 13). It is up‑regulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (14‑16). It functions as a cellular entry receptor for Hendra and Nipah viruses (17). Ephrin‑B2 is also important for the separation of the urinary and intestinal tracts during development (18).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.
  4. Bergemann, A.D. et al. (1995) Mol. Cell. Biol. 15:4921.
  5. Bennett, B.D. et al. (1995) Proc. Natl. Acad. Sci. 92:1866.
  6. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
  7. Zhao, J. et al. (2010) Mol. Pain 6:77.
  8. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
  9. Foo, S.S. et al. (2006) Cell 124:161.
  10. Wang, Y. et al. (2010) Nature 465:483.
  11. Sawamiphak, S. et al. (2010) Nature 465:487.
  12. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
  13. Yu, G. et al. (2003) J. Immunol. 171:106.
  14. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
  15. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
  16. Liu, W. et al. (2004) Br. J. Cancer 90:1620.
  17. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
  18. Dravis, C. et al. (2004) Dev. Biol. 271:272.

Customers Who Viewed This Item Also Viewed...

AF446
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
AF3034
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF395
Species: Hu
Applications: WB, IHC
AF641
Species: Mu
Applications: WB, IHC
AF432
Species: Mu
Applications: WB, Flow, CyTOF-ready, ICC
AF611
Species: Mu
Applications: WB
NBP2-20486
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB600-892
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
374-EA
Species: Hu
AF369
Species: Hu
Applications: WB, IHC, ICC
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
MAB3035
Species: Hu
Applications: Flow, CyTOF-ready, ICC, KO
496-EB
Species: Mu
Applications: Binding Activity

Publications for Ephrin-B2 (496-EB)(33)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Avian - Quail, N/A, Virus.

We have publications tested in 6 applications: Affinity Chromatography, Binding Assay, Bioassay, EnzAct, IHC Frozen, In Vivo.


Filter By Application
Affinity Chromatography
(1)
Binding Assay
(1)
Bioassay
(23)
EnzAct
(1)
IHC Frozen
(1)
In Vivo
(6)
All Applications
Filter By Species
Human
(10)
Mouse
(17)
Rat
(3)
Avian - Quail
(1)
N/A
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using 496-EB Applications Species
JM Baek, YH Cheon, SC Kwak, HY Jun, KH Yoon, MS Lee, JY Kim Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling Exp. Mol. Med., 2018;50(4):50. 2018 [PMID: 29700355] (Bioassay, Mouse) Bioassay Mouse
B Jakob, G Kochlamaza, M Jäpel, A Gauhar, HH Bock, T Maritzen, V Haucke Intersectin 1 is a component of the Reelin pathway to regulate neuronal migration and synaptic plasticity in the hippocampus Proc. Natl. Acad. Sci. U.S.A., 2017;114(21):5533-5538. 2017 [PMID: 28484035] (Bioassay, Mouse) Bioassay Mouse
The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model Mol Psychiatry, 2016;0(0):. 2016 [PMID: 27646263] (Bioassay, Rat) Bioassay Rat
Miyamoto T, Kim D, Knox J, Johnson E, Mucke L Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-beta-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity. J Biol Chem, 2016;291(4):1719-34. 2016 [PMID: 26589795] (Bioassay, Mouse) Bioassay Mouse
Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation. PLoS ONE, 2015;10(9):e0138835. 2015 [PMID: 26407069] (Bioassay, Mouse) Bioassay Mouse
Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015;6(0):6625. 2015 [PMID: 25865237] (EnzAct, Mouse) EnzAct Mouse
Lisle J, Mertens-Walker I, Stephens C, Stansfield S, Clements J, Herington A, Stephenson S Murine, but not human, ephrin-B2 can be efficiently cleaved by the serine protease kallikrein-4: implications for xenograft models of human prostate cancer. Exp Cell Res, 2015;333(1):136-46. 2015 [PMID: 25724897] (Bioassay, Mouse) Bioassay Mouse
Liu Q, Bradel-Tretheway B, Monreal A, Saludes J, Lu X, Nicola A, Aguilar H Nipah virus attachment glycoprotein stalk C-terminal region links receptor binding to fusion triggering. J Virol, 2015;89(3):1838-50. 2015 [PMID: 25428863] (Bioassay, Virus) Bioassay Virus
Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R The composition of EphB2 clusters determines the strength in the cellular repulsion response. J Cell Biol, 2014;204(3):409-22. 2014 [PMID: 24469634] (Bioassay, Human) Bioassay Human
Conway A, Schaffer D Biomaterial microenvironments to support the generation of new neurons in the adult brain. Stem Cells, 2014;32(5):1220-9. 2014 [PMID: 24449485] (Bioassay, N/A) Bioassay N/A
Show All 33 Publications.

Reviews for Ephrin-B2 (496-EB) (0)

There are no reviews for Ephrin-B2 (496-EB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Ephrin-B2 (496-EB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Ephrin-B2 (496-EB)

Discover related pathways, diseases and genes to Ephrin-B2 (496-EB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ephrin-B2 (496-EB)

Discover more about diseases related to Ephrin-B2 (496-EB).
 

Pathways for Ephrin-B2 (496-EB)

View related products by pathway.

PTMs for Ephrin-B2 (496-EB)

Learn more about PTMs related to Ephrin-B2 (496-EB).

Blogs on Ephrin-B2.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Efnb2
Entrez
Uniprot