Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to compete with Biotinylated Recombinant Mouse Ephrin‑B2 Fc Chimera (Catalog # BT496) for binding with immobilized Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2) in a functional ELISA assay.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-B2 protein
Mouse Ephrin-B2
(Arg27-Ala227)
Accession # AAA82934
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Efnb2
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
49.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-65 kDa, reducing conditions
Publications
Read Publications using
496-EB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF

  • EFNB2
  • ELF-2
  • ephrin B2
  • EphrinB2
  • Ephrin-B2
  • EPLG5
  • HTKL
  • Htk-L
  • LERK5
  • LERK-5
  • NLERK-1

Background

Ephrin‑B2, also known as Htk‑L, ELF‑2, LERK‑5, and NLERK‑1, is a 40 kDa member of the Ephrin‑B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin‑B ligands are structurally related to GPI‑anchored Ephrin‑A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑B2 preferentially interacts with receptors in the EphB family (1, 2). Mature mouse Ephrin‑B2 consists of a 204 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain [Cerretti 1197, Bergemann 4921, Bennett 1866]. Within the ECD, mouse Ephrin‑B2 shares 97% and 98% aa sequence identity with human and rat Ephrin‑B2, respectively. Ephrin‑B2 is expressed presynaptically on neurons (6, 7). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (6‑8). Ephrin‑B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (9, 10). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (8‑11). Ephrin‑B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (12, 13). It is up‑regulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (14‑16). It functions as a cellular entry receptor for Hendra and Nipah viruses (17). Ephrin‑B2 is also important for the separation of the urinary and intestinal tracts during development (18).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.
  4. Bergemann, A.D. et al. (1995) Mol. Cell. Biol. 15:4921.
  5. Bennett, B.D. et al. (1995) Proc. Natl. Acad. Sci. 92:1866.
  6. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
  7. Zhao, J. et al. (2010) Mol. Pain 6:77.
  8. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
  9. Foo, S.S. et al. (2006) Cell 124:161.
  10. Wang, Y. et al. (2010) Nature 465:483.
  11. Sawamiphak, S. et al. (2010) Nature 465:487.
  12. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
  13. Yu, G. et al. (2003) J. Immunol. 171:106.
  14. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
  15. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
  16. Liu, W. et al. (2004) Br. J. Cancer 90:1620.
  17. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
  18. Dravis, C. et al. (2004) Dev. Biol. 271:272.

Publications for Ephrin-B2 (496-EB)(46)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Avian - Quail, Chicken, Primate - Chlorocebus aethiops (African Green Monkey), Virus, Virus - Ghana, Virus - Henipavirus.

We have publications tested in 14 applications: Binding Assay, Bioassay, Cell Culture, Co-Immunoprecipitation, Coprecipitation Assay, ELISA Capture, Enzyme Assay, Flow Cytometry, ICC, IHC, IHC-Fr, Immunoprecipitation, In Vivo, Western Blot.


Filter By Application
Binding Assay
(1)
Bioassay
(31)
Cell Culture
(1)
Co-Immunoprecipitation
(1)
Coprecipitation Assay
(1)
ELISA Capture
(1)
Enzyme Assay
(1)
Flow Cytometry
(1)
ICC
(1)
IHC
(2)
IHC-Fr
(1)
Immunoprecipitation
(2)
In Vivo
(7)
Western Blot
(1)
All Applications
Filter By Species
Human
(13)
Mouse
(26)
Rat
(4)
Avian - Quail
(1)
Chicken
(2)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Virus
(1)
Virus - Ghana
(1)
Virus - Henipavirus
(1)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using 496-EB Applications Species
Chang, C;Banerjee, SL;Park, SS;Zhang, X;Cotnoir-White, D;Desbois, M;Grill, B;Kania, A; Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function bioRxiv : the preprint server for biology 2023-08-29 [PMID: 37693478] (Bioassay, Human, Chicken) Bioassay Human, Chicken
M Yang, W Zhu, T Truong, B Pickering, S Babiuk, D Kobasa, L Banadyga Detection of Nipah and Hendra Viruses Using Recombinant Human Ephrin B2 Capture Virus in Immunoassays Viruses, 2022-07-28;14(8):. 2022-07-28 [PMID: 36016279] (ELISA Capture, Mouse) ELISA Capture Mouse
S Bhatia, D Nguyen, LB Darragh, B Van Court, J Sharma, MW Knitz, M Piper, S Bukkapatna, J Gadwa, TE Bickett, S Bhuvane, S Corbo, B Wu, Y Lee, M Fujita, M Joshi, LE Heasley, RL Ferris, O Rodriguez, C Albanese, M Kapoor, EB Pasquale, SD Karam EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment Nature Communications, 2022-06-20;13(1):3535. 2022-06-20 [PMID: 35725568] (Bioassay, Mouse) Bioassay Mouse
V Ortega, JLR Zamora, IA Monreal, DT Hoffman, S Ezzatpour, GP Johnston, EM Contreras, FJ Vilchez-De, HC Aguilar Novel Roles of the Nipah Virus Attachment Glycoprotein and Its Mobility in Early and Late Membrane Fusion Steps MBio, 2022-05-04;0(0):e0322221. 2022-05-04 [PMID: 35506666] (Bioassay, Mouse) Bioassay Mouse
D Chen, ED Hughes, TL Saunders, J Wu, MN Hernández, T Makinen, PD King Angiogenesis depends upon EPHB4-mediated export of collagen IV from vascular endothelial cells JCI Insight, 2022-02-22;0(0):. 2022-02-22 [PMID: 35015735] (Bioassay, Mouse) Bioassay Mouse
C Donato, L Kunz, F Castro-Gin, A Paasinen-S, K Strittmatt, BM Szczerba, R Scherrer, N Di Maggio, W Heusermann, O Biehlmaier, C Beisel, M Vetter, C Rochlitz, WP Weber, A Banfi, T Schroeder, N Aceto Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Rep, 2020-09-08;32(10):108105. 2020-09-08 [PMID: 32905777] (Western Blot, Human, Mouse) Western Blot Human, Mouse
YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo Oxid Med Cell Longev, 2020-06-05;2020(0):1404915. 2020-06-05 [PMID: 32587656] (Bioassay, Mouse) Bioassay Mouse
ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus) Coprecipitation Assay Virus - Henipavirus
K Voigt, M Hoffmann, JF Drexler, MA Müller, C Drosten, G Herrler, N Krüger Fusogenicity of the Ghana Virus (Henipavirus: Ghanaian bat henipavirus) Fusion Protein is Controlled by the Cytoplasmic Domain of the Attachment Glycoprotein Viruses, 2019-08-29;11(9):. 2019-08-29 [PMID: 31470664] (Immunoprecipitation, Virus - Ghana) Immunoprecipitation Virus - Ghana
D Herrero, S Cañón, G Albericio, RM Carmona, S Aguilar, S Mañes, A Bernad Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart Redox Biol, 2019-03-04;22(0):101156. 2019-03-04 [PMID: 30851670] (Bioassay, Flow Cytometry, Mouse) Bioassay, Flow Cytometry Mouse
Show All 46 Publications.

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Bioinformatics

Gene Symbol Efnb2
Uniprot