Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

Order Details

Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to compete with Biotinylated Recombinant Mouse Ephrin‑B2 Fc Chimera (Catalog # BT496) for binding with immobilized Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2) in a functional ELISA assay.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived mouse Ephrin-B2 protein
Mouse Ephrin-B2
Accession # AAA82934
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
49.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60-65 kDa, reducing conditions
Read Publications using
496-EB in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF

  • EFNB2
  • ELF-2
  • ephrin B2
  • EphrinB2
  • Ephrin-B2
  • EPLG5
  • HTKL
  • Htk-L
  • LERK5
  • LERK-5
  • NLERK-1


Ephrin‑B2, also known as Htk‑L, ELF‑2, LERK‑5, and NLERK‑1, is a 40 kDa member of the Ephrin‑B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin‑B ligands are structurally related to GPI‑anchored Ephrin‑A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑B2 preferentially interacts with receptors in the EphB family (1, 2). Mature mouse Ephrin‑B2 consists of a 204 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain [Cerretti 1197, Bergemann 4921, Bennett 1866]. Within the ECD, mouse Ephrin‑B2 shares 97% and 98% aa sequence identity with human and rat Ephrin‑B2, respectively. Ephrin‑B2 is expressed presynaptically on neurons (6, 7). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (6‑8). Ephrin‑B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (9, 10). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (8‑11). Ephrin‑B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (12, 13). It is up‑regulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (14‑16). It functions as a cellular entry receptor for Hendra and Nipah viruses (17). Ephrin‑B2 is also important for the separation of the urinary and intestinal tracts during development (18).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.
  4. Bergemann, A.D. et al. (1995) Mol. Cell. Biol. 15:4921.
  5. Bennett, B.D. et al. (1995) Proc. Natl. Acad. Sci. 92:1866.
  6. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
  7. Zhao, J. et al. (2010) Mol. Pain 6:77.
  8. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
  9. Foo, S.S. et al. (2006) Cell 124:161.
  10. Wang, Y. et al. (2010) Nature 465:483.
  11. Sawamiphak, S. et al. (2010) Nature 465:487.
  12. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
  13. Yu, G. et al. (2003) J. Immunol. 171:106.
  14. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
  15. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
  16. Liu, W. et al. (2004) Br. J. Cancer 90:1620.
  17. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
  18. Dravis, C. et al. (2004) Dev. Biol. 271:272.

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Publications for Ephrin-B2 (496-EB)(33)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Avian - Quail, N/A, Virus.

We have publications tested in 6 applications: Affinity Chromatography, Binding Assay, Bioassay, EnzAct, IHC Frozen, In Vivo.

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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using 496-EB Applications Species
JM Baek, YH Cheon, SC Kwak, HY Jun, KH Yoon, MS Lee, JY Kim Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling Exp. Mol. Med., 2018;50(4):50. 2018 [PMID: 29700355] (Bioassay, Mouse) Bioassay Mouse
B Jakob, G Kochlamaza, M Jäpel, A Gauhar, HH Bock, T Maritzen, V Haucke Intersectin 1 is a component of the Reelin pathway to regulate neuronal migration and synaptic plasticity in the hippocampus Proc. Natl. Acad. Sci. U.S.A., 2017;114(21):5533-5538. 2017 [PMID: 28484035] (Bioassay, Mouse) Bioassay Mouse
The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model Mol Psychiatry, 2016;0(0):. 2016 [PMID: 27646263] (Bioassay, Rat) Bioassay Rat
Miyamoto T, Kim D, Knox J, Johnson E, Mucke L Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-beta-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity. J Biol Chem, 2016;291(4):1719-34. 2016 [PMID: 26589795] (Bioassay, Mouse) Bioassay Mouse
Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation. PLoS ONE, 2015;10(9):e0138835. 2015 [PMID: 26407069] (Bioassay, Mouse) Bioassay Mouse
Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015;6(0):6625. 2015 [PMID: 25865237] (EnzAct, Mouse) EnzAct Mouse
Lisle J, Mertens-Walker I, Stephens C, Stansfield S, Clements J, Herington A, Stephenson S Murine, but not human, ephrin-B2 can be efficiently cleaved by the serine protease kallikrein-4: implications for xenograft models of human prostate cancer. Exp Cell Res, 2015;333(1):136-46. 2015 [PMID: 25724897] (Bioassay, Mouse) Bioassay Mouse
Liu Q, Bradel-Tretheway B, Monreal A, Saludes J, Lu X, Nicola A, Aguilar H Nipah virus attachment glycoprotein stalk C-terminal region links receptor binding to fusion triggering. J Virol, 2015;89(3):1838-50. 2015 [PMID: 25428863] (Bioassay, Virus) Bioassay Virus
Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R The composition of EphB2 clusters determines the strength in the cellular repulsion response. J Cell Biol, 2014;204(3):409-22. 2014 [PMID: 24469634] (Bioassay, Human) Bioassay Human
Conway A, Schaffer D Biomaterial microenvironments to support the generation of new neurons in the adult brain. Stem Cells, 2014;32(5):1220-9. 2014 [PMID: 24449485] (Bioassay, N/A) Bioassay N/A
Show All 33 Publications.

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Gene Symbol Efnb2