Recombinant Mouse EphA1 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse EphA1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmEphA1 at 2 µg/mL (100 µL/well) can bind rmEphrin-A1/Fc Chimera with a linear range of 1.25-80 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse EphA1 protein
  Glu27-Glu548, with a C-terminal 6-His tag
• Accession #: AAH71215
• N-terminal Sequence: Glu27
• Protein/Peptide Type: Recombinant Proteins
• Gene: Epha1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 57.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EphA1 Protein, CF

• EC 2.7.10
• EC 2.7.10.1
• EPH receptor A1
• eph tyrosine kinase 1
• EphA1
• EPHephrin type-A receptor 1
• EPHT
• EPHT1erythropoietin-producing hepatoma amplified sequence
• Esk
• MGC163163
• oncogene EPH
• soluble EPHA1 variant 1
• soluble EPHA1 variant 2
• Tyrosine-protein kinase receptor EPH

Background

EphA1, also known as Eph and Esk, is a member of the Eph receptor tyrosine kinase family and binds several Ephrin-A ligands. The A and B class Eph proteins share a common structural organization (1 - 4). The mouse EphA1 cDNA encodes a 977 amino acid (aa) precursor that includes a 24 aa signal sequence, a 524 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 408 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5, 6). Within the ECD, mouse EphA1 shares 84% aa sequence identity with human EphA1, approximately 40% aa sequence identity with mouse EphA2, 3, 4, 6, 7, and 8, and 27% aa sequence identity with mouse EphA5. A splice variant of mouse EphA1 lacks the transmembrane segment and is predicted to exist as a soluble molecule (7). Membrane bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation (7). Reverse signaling is propagated through the Ephrin ligand. EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes (5, 7). It is expressed on CD4⁺ T cells but not on CD19⁺ B cells (8). On T cells, EphA1 induces Tyr phosphorylation of PYK2 and promotes chemokine-induced chemotaxis (8). EphA1 is upregulated or downregulated in a variety of human carcinomas and is implicated in tumor invasiveness (2, 9, 10).

1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
5. Coulthard, M.G. et al. (2001) Growth Factors 18:303.
6. Lickliter, J.D. et al. (1996) Proc. Natl. Acad. Sci. 93:145.
7. Douville, E.M.J. et al. (1992) Mol. Cell. Biol. 12:2681.
8. Aasheim, H.-C. et al. (2005) Blood 105:2869.
9. Iwase, T. et al. (1993) Biochim. Biophys. Res. Commun. 194:698.
10. Hirai H. et al. (1987) Science 238:1717.

Bioinformatics

• Gene Symbol: Epha1
• Uniprot:
  • AAH71215()