Recombinant Mouse Endoglycan/PODXL2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Endoglycan/PODXL2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED₅₀ for this effect is 0.1-0.6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Endoglycan/PODXL2 protein
  Gly30-Gly498, with a C-terminal 6-His tag
• Accession #: XP_006506309
• N-terminal Sequence: Gly30
• Protein/Peptide Type: Recombinant Proteins
• Gene: Podxl2
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-124 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Endoglycan/PODXL2 Protein, CF

• Endoglycan
• PODLX2
• podocalyxin-like 2
• podocalyxin-like protein 2
• PODXL2

Background

Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature mouse Endoglycan consists of an extracellular domain (ECD) that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the ECD, mouse Endoglycan shares 96% and 75% amino acid identity with rat and human Endoglycan, respectively. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ‑domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na⁺/H⁺ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although, depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (2, 3, 5, 6).

1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
3. Nielsen, J.S. and K.M. McNagny (2008) J. Cell Sci. 121:3683.
4. Tan, P.C. et al. (2006) Stem Cells 24:1150.
5. Kerr, S.C. et al. (2008) J. Immunol. 181:1480.
6. Sarangapani, K.K. et al. (2004) J. Biol. Chem. 279:2291.

Bioinformatics

• Gene Symbol: Podxl2
• Uniprot:
  • XP_006506309()