Recombinant Mouse EGF Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse EGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.
Source
E. coli-derived mouse EGF protein
Asn977-Arg1029, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Egf
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
6.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2028-EG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EGF Protein, CF

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF‑like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Gray, A. et al. (1983) Nature 303:722.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

    Publications for EGF (2028-EG)(163)

    We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Rhesus Macaque, Rhinoceros, Transgenic Mouse.

    We have publications tested in 5 applications: Bioassay, Cell Culture, In Vivo, Organoid Culture, Tissue Culture.


    Filter By Application
    Bioassay
    (143)
    Cell Culture
    (13)
    In Vivo
    (4)
    Organoid Culture
    (1)
    Tissue Culture
    (1)
    All Applications
    Filter By Species
    Human
    (10)
    Mouse
    (143)
    Rat
    (2)
    Chicken
    (2)
    Rhesus Macaque
    (1)
    Rhinoceros
    (1)
    Transgenic Mouse
    (5)
    All Species
    Showing Publications 1 - 10 of 163. Show All 163 Publications.
    Publications using 2028-EG Applications Species
    Pereira, M;Glogova, A;Haagsma, J;Stewart, J;Shepherd, TG;Petrik, J; Mutant p53 murine oviductal epithelial cells induce progression of high-grade serous carcinoma and are most sensitive to simvastatin therapy in vitro and in vivo Journal of ovarian research 2023-11-20 [PMID: 37986175] (Bioassay, Mouse) Bioassay Mouse
    Read, E;Peña-Cearra, A;Coman, D;Jowett, GM;W H Chung, M;Coales, I;Syntaka, S;Finlay, RE;Tachó-Piñot, R;van Der Post, S;Naizi, U;Roberts, LB;Hepworth, MR;Curtis, MA;Neves, JF; Bi-directional signalling between the intestinal epithelium and type-3 innate lymphoid cells (ILC3) regulates secretory dynamics and IL-22 Mucosal immunology 2023-11-10 [PMID: 37952849] (Bioassay, Mouse) Bioassay Mouse
    Hung, YH;Capeling, M;Villanueva, JW;Kanke, M;Shanahan, MT;Huang, S;Cubitt, R;Rinaldi, VD;Schimenti, JC;Spence, JR;Sethupathy, P; Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model BMC genomics 2023-10-26 [PMID: 37884859] (Mouse) Mouse
    Korwin-Mihavics, BR;Dews, EA;Martorelli di Genova, B;Huston, CD; Organoid-based in vitro systems to model Cryptosporidium parvum infection in 2D and 3D bioRxiv : the preprint server for biology 2023-09-29 [PMID: 37808810] (Bioassay, Mouse) Bioassay Mouse
    Tresenrider, A;Hooper, M;Todd, L;Kierney, F;Blasdel, N;Trapnell, C;Reh, TA; A multiplexed, single-cell sequencing screen identifies compounds that increase neurogenic reprogramming of murine Muller glia bioRxiv : the preprint server for biology 2023-09-27 [PMID: 37808650] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
    Williams, C;Brown, R;Zhao, Y;Wang, J;Chen, Z;Blunt, K;Pilat, J;Parang, B;Choksi, Y;Lau, K;Hiebert, S;Short, S;Jacobse, J;Xu, Y;Yang, Y;Goettel, J; MTGR1 is required to maintain small intestinal stem cell populations Research square 2023-09-21 [PMID: 37790452] (Bioassay, Mouse) Bioassay Mouse
    Loxha, L;Ibrahim, NK;Stasche, AS;Cinar, B;Dolgner, T;Niessen, J;Schreek, S;Fehlhaber, B;Forster, M;Stanulla, M;Hinze, L; GSK3? Regulates Temporally Dynamic Changes in Ribosomal Proteins upon Amino Acid Starvation in Cancer Cells International journal of molecular sciences 2023-08-26 [PMID: 37686063] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
    S Scharaw, A Sola-Carva, I Belevich, AT Webb, S Das, S Andersson, N Pentinmikk, EJ Villablanc, JR Goldenring, E Jokitalo, RJ Coffey, P Katajisto Golgi organization is a determinant of stem cell function in the small intestine bioRxiv : the preprint server for biology, 2023-03-24;0(0):. 2023-03-24 [PMID: 36993731] (Bioassay, Mouse) Bioassay Mouse
    L Sun, Y Zhang, J Cai, B Rimal, ER Rocha, JP Coleman, C Zhang, RG Nichols, Y Luo, B Kim, Y Chen, KW Krausz, CC Harris, AD Patterson, Z Zhang, S Takahashi, FJ Gonzalez Bile salt hydrolase in non-enterotoxigenic Bacteroides potentiates colorectal cancer Nature Communications, 2023-02-10;14(1):755. 2023-02-10 [PMID: 36765047] (Bioassay, Mouse) Bioassay Mouse
    FM Yavitt, BE Kirkpatric, MR Blatchley, KF Speckl, E Mohagheghi, R Moldovan, N Wang, PJ Dempsey, KS Anseth In situ modulation of intestinal organoid epithelial curvature through photoinduced viscoelasticity directs crypt morphogenesis Science Advances, 2023-01-20;9(3):eadd5668. 2023-01-20 [PMID: 36662859] (Bioassay, Mouse) Bioassay Mouse
    Show All 163 Publications.

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    Bioinformatics

    Gene Symbol Egf
    Uniprot