Recombinant Mouse Dkk-1 Protein


Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 5897‑DK) inhibits a constant dose more
1 µg/lane of Recombinant Mouse Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 35-40 kDa. Multiple bands in gel are due to variable glycosylation.

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Recombinant Mouse Dkk-1 Protein Summary

Details of Functionality
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 (Catalog # 5897-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 10-60 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived mouse Dkk-1 protein
Accession #
N-terminal Sequence
Ser30 & Thr32
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
35-40 kDa, reducing conditions
Read Publications using
5897-DK in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Dkk-1 Protein

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like


Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

  1. Glinka, A. et al. (1998) Nature 391:357.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

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Publications for Dkk-1 (5897-DK)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.

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Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using 5897-DK Applications Species
HE Ahrens, J Huettemeis, M Schmidt, C Kaether, J von Maltza Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle Skelet Muscle, 2018;8(1):20. 2018 [PMID: 29973273] (Bioassay, Mouse) Bioassay Mouse
R Seitz, G Weber, S Albrecht, R Fuchshofer, ER Tamm, A Ohlmann Cross-Inhibition of Norrin and TGF-? Signaling Modulates Development of Retinal and Choroidal Vasculature Invest. Ophthalmol. Vis. Sci., 2018;59(6):2240-2251. 2018 [PMID: 29715368] (Bioassay, Mouse) Bioassay Mouse
SP Ross, KE Baker, A Fisher, L Hoff, ES Pak, AK Murashov miRNA-431 Prevents Amyloid-?-Induced Synapse Loss in Neuronal Cell Culture Model of Alzheimer&#039;s Disease by Silencing Kremen1 Front Cell Neurosci, 2018;12(0):87. 2018 [PMID: 29643768] (Bioassay, Mouse) Bioassay Mouse
D Gu, S Wang, S Zhang, P Zhang, G Zhou Directed transdifferentiation of M�ller glial cells to photoreceptors using the sonic hedgehog signaling pathway agonist purmorphamine Mol Med Rep, 2017;0(0):. 2017 [PMID: 28983586] (Bioassay, Rat) Bioassay Rat
MW Susman, EP Karuna, RC Kunz, TS Gujral, AV Cantú, SS Choi, BY Jong, K Okada, MK Scales, J Hum, LS Hu, MW Kirschner, R Nishinakam, S Yamada, DJ Laird, LE Jao, SP Gygi, ME Greenberg, HH Ho Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates Elife, 2017;6(0):. 2017 [PMID: 28885975] (Bioassay, Mouse) Bioassay Mouse
NG Tassew, J Charish, AP Shabanzade, V Luga, H Harada, N Farhani, P D'Onofrio, B Choi, A Ellabban, PEB Nickerson, VA Wallace, PD Koeberle, JL Wrana, PP Monnier Exosomes Mediate Mobilization of Autocrine Wnt10b to Promote Axonal Regeneration in the Injured CNS Cell Rep, 2017;20(1):99-111. 2017 [PMID: 28683327] (Bioassay, Mouse) Bioassay Mouse
T Tammela, FJ Sanchez-Ri, NM Cetinbas, K Wu, NS Joshi, K Helenius, Y Park, R Azimi, NR Kerper, RA Wesselhoef, X Gu, L Schmidt, M Cornwall-B, ÖH Yilmaz, W Xue, P Katajisto, A Bhutkar, T Jacks A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma Nature, 2017;545(7654):355-359. 2017 [PMID: 28489818] (Bioassay, Mouse) Bioassay Mouse
K Wilk, SA Yeh, LJ Mortensen, S Ghaffariga, CM Lombardo, SH Bassir, ZA Aldawood, CP Lin, G Intini Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28366454] (Bioassay, Mouse) Bioassay Mouse
J Messéant, J Ezan, P Delers, K Glebov, C Marchiol, F Lager, G Renault, F Tissir, M Montcouqui, N Sans, C Legay, L Strochlic Wnts contribute to neuromuscular junction formation through distinct signaling pathways Development, 2017;0(0):. 2017 [PMID: 28348167] (In Vivo, Mouse) In Vivo Mouse
J Zhang, J Zhang, C Qi, P Yang, X Chen, Y Liu Activation of Wnt3?/?-catenin signal pathway attenuates apoptosis of the cerebral microvascular endothelial cells induced by oxygen-glucose deprivation Biochem. Biophys. Res. Commun, 2017;0(0):. 2017 [PMID: 28347817] (Bioassay, Mouse) Bioassay Mouse
Show All 36 Publications.

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Blogs on Dkk-1.

The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

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Gene Symbol Dkk1