Recombinant Mouse Dkk-1 Protein

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Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 5897‑DK) inhibits a constant dose ...read more
1 µg/lane of Recombinant Mouse Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 35-40 kDa. Multiple bands in gel are due to variable glycosylation.

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Dkk-1 Protein Summary

Details of Functionality
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 (Catalog # 5897-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 10-60 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Dkk-1 protein
Ser30-His272
Accession #
N-terminal Sequence
Ser30 & Thr32
Protein/Peptide Type
Recombinant Proteins
Gene
Dkk1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35-40 kDa, reducing conditions
Publications
Read Publications using
5897-DK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Dkk-1 Protein

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

  1. Glinka, A. et al. (1998) Nature 391:357.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

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Publications for Dkk-1 (5897-DK)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(32)
In Vivo
(4)
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Human
(2)
Mouse
(33)
Rat
(1)
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Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using 5897-DK Applications Species
HE Ahrens, J Huettemeis, M Schmidt, C Kaether, J von Maltza Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle Skelet Muscle, 2018;8(1):20. 2018 [PMID: 29973273] (Bioassay, Mouse) Bioassay Mouse
R Seitz, G Weber, S Albrecht, R Fuchshofer, ER Tamm, A Ohlmann Cross-Inhibition of Norrin and TGF-? Signaling Modulates Development of Retinal and Choroidal Vasculature Invest. Ophthalmol. Vis. Sci., 2018;59(6):2240-2251. 2018 [PMID: 29715368] (Bioassay, Mouse) Bioassay Mouse
SP Ross, KE Baker, A Fisher, L Hoff, ES Pak, AK Murashov miRNA-431 Prevents Amyloid-?-Induced Synapse Loss in Neuronal Cell Culture Model of Alzheimer&#039;s Disease by Silencing Kremen1 Front Cell Neurosci, 2018;12(0):87. 2018 [PMID: 29643768] (Bioassay, Mouse) Bioassay Mouse
D Gu, S Wang, S Zhang, P Zhang, G Zhou Directed transdifferentiation of M�ller glial cells to photoreceptors using the sonic hedgehog signaling pathway agonist purmorphamine Mol Med Rep, 2017;0(0):. 2017 [PMID: 28983586] (Bioassay, Rat) Bioassay Rat
MW Susman, EP Karuna, RC Kunz, TS Gujral, AV Cantú, SS Choi, BY Jong, K Okada, MK Scales, J Hum, LS Hu, MW Kirschner, R Nishinakam, S Yamada, DJ Laird, LE Jao, SP Gygi, ME Greenberg, HH Ho Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates Elife, 2017;6(0):. 2017 [PMID: 28885975] (Bioassay, Mouse) Bioassay Mouse
NG Tassew, J Charish, AP Shabanzade, V Luga, H Harada, N Farhani, P D'Onofrio, B Choi, A Ellabban, PEB Nickerson, VA Wallace, PD Koeberle, JL Wrana, PP Monnier Exosomes Mediate Mobilization of Autocrine Wnt10b to Promote Axonal Regeneration in the Injured CNS Cell Rep, 2017;20(1):99-111. 2017 [PMID: 28683327] (Bioassay, Mouse) Bioassay Mouse
T Tammela, FJ Sanchez-Ri, NM Cetinbas, K Wu, NS Joshi, K Helenius, Y Park, R Azimi, NR Kerper, RA Wesselhoef, X Gu, L Schmidt, M Cornwall-B, ÖH Yilmaz, W Xue, P Katajisto, A Bhutkar, T Jacks A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma Nature, 2017;545(7654):355-359. 2017 [PMID: 28489818] (Bioassay, Mouse) Bioassay Mouse
K Wilk, SA Yeh, LJ Mortensen, S Ghaffariga, CM Lombardo, SH Bassir, ZA Aldawood, CP Lin, G Intini Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28366454] (Bioassay, Mouse) Bioassay Mouse
J Messéant, J Ezan, P Delers, K Glebov, C Marchiol, F Lager, G Renault, F Tissir, M Montcouqui, N Sans, C Legay, L Strochlic Wnts contribute to neuromuscular junction formation through distinct signaling pathways Development, 2017;0(0):. 2017 [PMID: 28348167] (In Vivo, Mouse) In Vivo Mouse
J Zhang, J Zhang, C Qi, P Yang, X Chen, Y Liu Activation of Wnt3?/?-catenin signal pathway attenuates apoptosis of the cerebral microvascular endothelial cells induced by oxygen-glucose deprivation Biochem. Biophys. Res. Commun, 2017;0(0):. 2017 [PMID: 28347817] (Bioassay, Mouse) Bioassay Mouse
Show All 36 Publications.

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Bioinformatics

Gene Symbol Dkk1
Entrez
Uniprot