Recombinant Mouse Dermatopontin Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Dermatopontin Protein, CF Summary

• Details of Functionality: Measured by its ability to modulate collagen fibrillogenesis. 20 µg/mL of rmDermatopontin can significantly enhance the rate of collagen fibrillogenesis.
• Source: Mouse myeloma cell line, NS0-derived mouse Dermatopontin protein
  Gln19-Val201 with a C-terminal 6-His tag
• Accession #: Q9QZZ6
• N-terminal Sequence: No results obtained: Gln19 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: Dpt
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 22.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 23 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Dermatopontin Protein, CF

• Dermatopontin
• DPT
• Eq1
• TRAMP
• Tyrosine-rich acidic matrix protein

Background

Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein) and EQ-1 (early quiescence-1), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature mouse Dermatopontin shares 92% and 97% amino acid sequence identity with human and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6 - 8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan Decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).

1. Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
2. Tzen, C.Y. and Y.W. Huang (2004) Exp. Cell Res. 294:30.
3. Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
4. Cronshaw, A.D. et al. (1993) Matrix 13:255.
5. Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
6. MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
7. Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
8. Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
9. Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
10. Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
11. Okamoto, O. et al. (1996) J. Biochem. 119:106.
12. Okamoto, O. et al. (1999) Biochem. J. 337:537.
13. Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
14. Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.

Publications for Dermatopontin (5749-DP)(1)

Publication using 5749-DP

• AC Kramer, AL Blake, ME Taisto, MJ Lehrke, BR Webber, TC Lund Dermatopontin in Bone Marrow Extracellular Matrix Regulates Adherence but Is Dispensable for Murine Hematopoietic Cell Maintenance Stem Cell Reports, 2017-08-24;0(0):. 2017-08-24 [PMID: 28844660] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Dpt
• Uniprot:
  • Q9QZZ6(Mouse)