Recombinant Mouse Dectin-2/CLEC6A Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Dectin-2/CLEC6A Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated alpha -D-Mannose-PAA in a functional ELISA.
• Source: Mouse myeloma cell line, NS0-derived mouse Dectin-2/CLEC6A protein
  Phe43-Leu209, with an N-terminal 7-His tag and Ala
• Accession #: Q9JKF4.1
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: Clec4n
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 20.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 24 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Dectin-2/CLEC6A Protein, CF

• CLEC4N
• CLEC6A
• CLECSF10
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10
• C-type lectin domain family 6 member A
• C-type lectin domain family 6, member A
• C-type lectin superfamily member 10
• DC-associated C-type lectin 2
• dectin 2
• Dectin2
• Dectin-2
• Dendritic cell-associated C-type lectin 2

Background

Dectin-2 alpha , also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha , CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3 - 6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4⁺CD25⁺ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
3. Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
4. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
5. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
9. Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Publications for Dectin-2/CLEC6A (1525-DC)(2)

Publications using 1525-DC

• Li, J;Liu, XG;Ge, RL;Yin, YP;Liu, YD;Lu, WP;Huang, M;He, XY;Wang, J;Cai, G;Sun, SH;Yuan, JH; The ligation between ERMAP, galectin-9 and dectin-2 promotes Kupffer cell phagocytosis and antitumor immunity Nature immunology 2023-10-09 [PMID: 37813965] (ELISA Capture, Immunoprecipitation, N/A)
• C Leclaire, K Lecointe, PA Gunning, S Tribolo, DW Kavanaugh, A Wittmann, D Latousakis, DA MacKenzie, N Kawasaki, N Juge Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins FASEB J., 2018-01-29;0(0):fj201700619R. 2018-01-29 [PMID: 29401627] (Bioassay, Mass Spectrometry, Mouse)

Bioinformatics

• Gene Symbol: Clec4n
• Uniprot:
  • Q9JKF4.1()