Recombinant Mouse Decorin Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Decorin Protein, CF Summary

• Details of Functionality: Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. 5 µg/mL of Recombinant Mouse Decorin can significantly delay the rate of collagen fibrillogenesis.
• Source: Mouse myeloma cell line, NS0-derived mouse Decorin protein
  Gly17-Lys354 & Asp31-Lys354, with a C-terminal 10-His tag
• Accession #: P28654
• N-terminal Sequence: Gly17 & Asp31
• Protein/Peptide Type: Recombinant Proteins
• Gene: Dcn
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-45 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Decorin Protein, CF

• Bone proteoglycan II
• CSCD
• DCN
• decorin proteoglycan
• Decorin
• dermatan sulphate proteoglycans II
• DSPG2
• PG40
• PGII
• PG-II
• PGS2
• PG-S2
• proteoglycan core protein
• SLRR1B
• SLRR1BPGS2
• small leucine-rich protein 1B

Background

Decorin, also known as PG40 and PGS2, is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10 ‑ 12 tandem leucine-rich repeats (LRR) (1). The mouse Decorin cDNA encodes a 354 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide (2). Mature mouse Decorin contains twelve tandem LRR and shares 80% and 87% aa sequence identity with human and rat Decorin, respectively. Decorin is an N-glycosylated protein that also carries a variably-sized hybrid chondroitin/dermatan sulfate chain at Ser34 (2, 3). Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa (4). Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF-2, GDF-8/Myostatin, TGF-beta , and WISP-1 (3, 5 ‑ 8). It also binds and activates EGF R, ErbB4, and IGF-I R (9 ‑ 11). In vivo, Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression (12 ‑ 15). Decorin is cleared from the extracellular space by LRP‑mediated endocytosis (16).

1. Schaefer, L. and R.V. Iozzo (2008) J. Biol. Chem. 283:21305.
2. Scholzen, T. et al. (1994) J. Biol. Chem. 269:28270.
3. Zamfir, A. et al. (2003) Glycobiology 13:733.
4. Roughley, P.J. and R.J. White (1989) Biochem. J. 262:823.
5. Ferdous, Z. et al. (2007) J. Biol. Chem. 282:35887.
6. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
7. Cabello-Verrugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
8. Desnoyers, L. et al. (2001) J. Biol. Chem. 276:47599.
9. Zhu, J.-X. et al. (2005) J. Biol. Chem. 280:32468.
10. Santra, M. et al. (2000) J. Biol. Chem. 275:35153.
11. Schonherr, E. et al. (2005) J. Biol. Chem. 280:15767.
12. Olguin, H.C. et al. (2003) Dev. Biol. 259:209.
13. Schonherr, E. et al. (2004) J. Vasc. Res. 41:499.
14. Seidler, D.G. et al. (2006) J. Biol. Chem. 281:26408.
15. Reed, C.C. et al. (2005) Oncogene 24:1104.
16. Brandan, E. et al. (2006) J. Biol. Chem. 281:31562.

Publications for Decorin (1060-DE)(13)

Publications using 1060-DE

• Agrawal, A;Thomann, S;Basu, S;Grün, D; NiCo identifies extrinsic drivers of cell state modulation by niche covariation analysis Nature communications 2024-12-05 [PMID: 39639035] (Bioassay, Mouse)
• Chatterjee, J;Koleske, JP;Chao, A;Sauerbeck, AD;Chen, JK;Qi, X;Ouyang, M;Boggs, LG;Idate, R;Marco Y Marquez, LI;Kummer, TT;Gutmann, DH; Brain injury drives optic glioma formation through neuron-glia signaling Acta neuropathologica communications 2024-02-02 [PMID: 38308315] (In vivo assay, Mouse)
• Wang, J;Ocadiz-Ruiz, R;Hall, MS;Bushnell, GG;Orbach, SM;Decker, JT;Raghani, RM;Zhang, Y;Morris, AH;Jeruss, JS;Shea, LD; A synthetic metastatic niche reveals antitumor neutrophils drive breast cancer metastatic dormancy in the lungs Nature communications 2023-08-08 [PMID: 37553342] (Bioassay, Mouse)
• R Jiang, U Smailovic, H Haytural, BM Tijms, H Li, RM Haret, G Shevchenko, G Chen, A Abelein, J Gobom, S Frykman, M Sekiguchi, R Fujioka, N Watamura, H Sasaguri, S Nyström, P Hammarströ, TC Saido, V Jelic, S Syvänen, H Zetterberg, B Winblad, J Bergquist, PJ Visser, P Nilsson Increased CSF-decorin predicts brain pathological changes driven by Alzheimer&#039;s Abeta amyloidosis Acta neuropathologica communications, 2022-07-04;10(1):96. 2022-07-04 [PMID: 35787306] (Bioassay, Mouse)
• J Liu, S Zhu, L Zeng, J Li, DJ Klionsky, G Kroemer, J Jiang, D Tang, R Kang DCN released from ferroptotic cells ignites AGER-dependent immune responses Autophagy, 2021-12-29;0(0):1-14. 2021-12-29 [PMID: 34964698] (Bioassay, Mouse)
• J Chatterjee, S Sanapala, O Cobb, A Bewley, AK Goldstein, E Cordell, X Ge, JR Garbow, MJ Holtzman, DH Gutmann Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia Nature Communications, 2021-12-08;12(1):7122. 2021-12-08 [PMID: 34880260] (Bioassay, Mouse)
• AC Daquinag, Z Gao, C Fussell, K Sun, MG Kolonin Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly Cells, 2020-12-09;9(12):. 2020-12-09 [PMID: 33317052] (Bioassay, Mouse)
• NMP Cartee, MM Wang Binding of omeprazole to protein targets identified by monoclonal antibodies PLoS ONE, 2020-09-18;15(9):e0239464. 2020-09-18 [PMID: 32946534] (Mouse)
• M Zhao, I Mantel, E Gelize, X Li, X Xie, A Arboleda, M Seminel, R Levy-Boukr, M Dernigogho, A Prunotto, C Andrieu-So, C Rivolta, J Canonica, MC Naud, S Lechner, N Farman, I Bravo-Osun, R Herrero-Va, F Jaisser, F Behar-Cohe Mineralocorticoid receptor antagonism limits experimental choroidal neovascularization and structural changes associated with neovascular age-related macular degeneration Nat Commun, 2019-01-21;10(1):369. 2019-01-21 [PMID: 30664640] (In Vivo, Rat)
• AC Daquinag, A Dadbin, B Snyder, X Wang, AA Sahin, NT Ueno, MG Kolonin Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells Mol Ther Oncolytics, 2017-05-17;6(0):1-9. 2017-05-17 [PMID: 28607949] (Western Blot, Mouse)
• Sneddon , Julie B, Borowiak , Malgorza, Melton , Douglas Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 2012-10-07;491(7426):765-8. 2012-10-07 [PMID: 23041930] (Cell Culture, Human)
• Ichii , Michiko, Frank , Mark Bar, Iozzo , Renato V, Kincade , Paul W The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood, 2011-11-23;119(7):1683-92. 2011-11-23 [PMID: 22117039] (Bioassay, Mouse)
• Daquinag AC, Zhang Y, Amaya-Manzanares F, Simmons PJ, Kolonin MG An isoform of decorin is a resistin receptor on the surface of adipose progenitor cells. Cell Stem Cell, 2011-06-16;9(1):74-86. 2011-06-16 [PMID: 21683670] (Binding Assay, Mouse)

Bioinformatics

• Gene Symbol: Dcn
• Uniprot:
  • P28654()