Recombinant Mouse CXCL14/BRAK Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse CXCL14/BRAK Protein, CF Summary

Details of Functionality
Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 2-8 μg/mL.
Source
E. coli-derived mouse CXCL14/BRAK protein
Ser23-Glu99
Accession #
N-terminal Sequence
Ser23
Protein/Peptide Type
Recombinant Proteins
Gene
Cxcl14
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
9.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
730-XC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL14/BRAK Protein, CF

  • BMAC
  • Bolekine
  • BRAK
  • BRAKKEC
  • chemokine (C-X-C motif) ligand 14
  • Chemokine BRAK
  • CXCL14
  • CXC-X3
  • member 14 (BRAK)
  • MIP-2 gamma
  • MIP2G
  • MIP-2G
  • NJACKec
  • SCYB14MGC10687
  • Small-inducible cytokine B14

Background

CXCL14/BRAK, also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of the CXC chemokine superfamily (1-5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Mouse CXCL14 shares approximately 30% aa sequence identity with mouse MIP‑2. Unlike MIP‑2, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E2 or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important in regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

  1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
  2. Cao, X. et al. (2000) J. Immunol. 165:2588.
  3. Kurth, I. et al. (2001) J. Exp. Med. 194:855.
  4. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
  5. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.

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Publications for CXCL14/BRAK (730-XC)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
All Applications
Filter By Species
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 730-XC Applications Species
Y Wang, Q Sun, Y Ye, X Sun, S Xie, Y Zhan, J Song, X Fan, B Zhang, M Yang, L Lv, K Hosaka, Y Yang, G Nie FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis JCI Insight, 2022;0(0):. 2022 [PMID: 35439170] (Bioassay, Mouse) Bioassay Mouse
Augsten M, Sjoberg E, Frings O, Vorrink S, Frijhoff J, Olsson E, Borg A, Ostman A Cancer-associated fibroblasts expressing CXCL14 rely upon NOS1-derived nitric oxide signaling for their tumor-supporting properties. Cancer Res, 2014;74(11):2999-3010. 2014 [PMID: 24710408] (Bioassay, Mouse) Bioassay Mouse
Kuang H, Chen Q, Fan X, Zhang Y, Zhang L, Peng H, Cao Y, Duan E CXCL14 inhibits trophoblast outgrowth via a paracrine/autocrine manner during early pregnancy in mice. J. Cell. Physiol., 2009;221(2):448-57. 2009 [PMID: 19626669] (Bioassay, Mouse) Bioassay Mouse
Takahashi M, Takahashi Y, Takahashi K, Zolotaryov FN, Hong KS, Iida K, Okimura Y, Kaji H, Chihara K CXCL14 enhances insulin-dependent glucose uptake in adipocytes and is related to high-fat diet-induced obesity. Biochem. Biophys. Res. Commun., 2007;364(4):1037-42. 2007 [PMID: 17971304] (Bioassay, Mouse) Bioassay Mouse
Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J Novel regulators of kidney development from the tips of the ureteric bud. J. Am. Soc. Nephrol., 2005;16(7):1993-2002. 2005 [PMID: 15917337] (Bioassay, Rat) Bioassay Rat
Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2005;105(1):31-9. 2005 [PMID: 15358618] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Cxcl14
Uniprot