Recombinant Mouse CXCL14/BRAK Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CXCL14/BRAK Protein, CF Summary

• Details of Functionality: Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED₅₀ for this effect is 2-8 μg/mL.
• Source: E. coli-derived mouse CXCL14/BRAK protein
  Ser23-Glu99
• Accession #: Q9JHH7
• N-terminal Sequence: Ser23
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cxcl14
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 9.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL14/BRAK Protein, CF

• BMAC
• Bolekine
• BRAK
• BRAKKEC
• chemokine (C-X-C motif) ligand 14
• Chemokine BRAK
• CXCL14
• CXC-X3
• member 14 (BRAK)
• MIP-2 gamma
• MIP2G
• MIP-2G
• NJACKec
• SCYB14MGC10687
• Small-inducible cytokine B14

Background

CXCL14/BRAK, also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of the CXC chemokine superfamily (1-5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Mouse CXCL14 shares approximately 30% aa sequence identity with mouse MIP‑2. Unlike MIP‑2, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E₂ or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important in regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
2. Cao, X. et al. (2000) J. Immunol. 165:2588.
3. Kurth, I. et al. (2001) J. Exp. Med. 194:855.
4. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
5. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.

Publications for CXCL14/BRAK (730-XC)(7)

Publications using 730-XC

• He, P;Wang, H;Cheng, S;Hu, F;Zhang, L;Chen, W;Xu, Y;Zhang, Y;Gu, Y;Li, Z;Jin, Y;Liu, X;Jia, Y; Geniposide ameliorates atherosclerosis by regulating macrophage polarization via perivascular adipocyte-derived CXCL14 Journal of ethnopharmacology 2023-05-04 [PMID: 37149071] (Bioassay, Mouse)
• Y Wang, Q Sun, Y Ye, X Sun, S Xie, Y Zhan, J Song, X Fan, B Zhang, M Yang, L Lv, K Hosaka, Y Yang, G Nie FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis JCI Insight, 2022-05-23;0(0):. 2022-05-23 [PMID: 35439170] (Bioassay, Mouse)
• Augsten M, Sjoberg E, Frings O, Vorrink S, Frijhoff J, Olsson E, Borg A, Ostman A Cancer-associated fibroblasts expressing CXCL14 rely upon NOS1-derived nitric oxide signaling for their tumor-supporting properties. Cancer Res, 2014-04-07;74(11):2999-3010. 2014-04-07 [PMID: 24710408] (Bioassay, Mouse)
• Kuang H, Chen Q, Fan X, Zhang Y, Zhang L, Peng H, Cao Y, Duan E CXCL14 inhibits trophoblast outgrowth via a paracrine/autocrine manner during early pregnancy in mice. J. Cell. Physiol., 2009-11-01;221(2):448-57. 2009-11-01 [PMID: 19626669] (Bioassay, Mouse)
• Takahashi M, Takahashi Y, Takahashi K, Zolotaryov FN, Hong KS, Iida K, Okimura Y, Kaji H, Chihara K CXCL14 enhances insulin-dependent glucose uptake in adipocytes and is related to high-fat diet-induced obesity. Biochem. Biophys. Res. Commun., 2007-10-29;364(4):1037-42. 2007-10-29 [PMID: 17971304] (Bioassay, Mouse)
• Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J Novel regulators of kidney development from the tips of the ureteric bud. J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002. 2005-05-25 [PMID: 15917337] (Bioassay, Rat)
• Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Cxcl14
• Uniprot:
  • Q9JHH7()