Recombinant Mouse CXCL11/I-TAC Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse CXCL11/I-TAC Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED₅₀ for this effect is 8.00‑96.0 ng/mL.
• Source: E. coli-derived mouse CXCL11/I-TAC protein
  Phe22-Met100
• Accession #: Q9JHH5
• N-terminal Sequence: Phe22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cxcl11
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 9.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL11/I-TAC Protein

• beta-R1
• b-R1
• chemokine (C-X-C motif) ligand 11
• CXCL11
• H174
• H174IP9
• Interferon gamma-inducible protein 9
• Interferon-inducible T-cell alpha chemoattractant
• IP-9member 11
• ITAC
• I-TAC
• I-TACMGC102770
• SCYB9B
• small inducible cytokine subfamily B (Cys-X-Cys), member 9B
• Small-inducible cytokine B11

Background

CXCL11, also known as I-TAC, SCYB9B, H174, IP-9, and beta -R1, is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to yield a 79 aa residue mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (Crg-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.

1. Widney, D.P. et al. (2000) J. Immunol. 164:6322.
2. Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
3. Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.

Publications for CXCL11/I-TAC (572-MC)(6)

Publications using 572-MC

• Hwang, SH;Yang, Y;Jung, JH;Kim, JW;Kim, Y; Stearoyl-CoA desaturase in CD4+ T cells suppresses tumor growth through activation of the CXCR3/CXCL11 axis in CD8+ T cells Cell & bioscience 2024-11-14 [PMID: 39543650] (Bioassay, Mouse)
• A Fumagalli, J Heuninck, A Pizzoccaro, E Moutin, J Koenen, M Séveno, T Durroux, MP Junier, G Schlecht-L, F Bachelerie, D Schütz, R Stumm, MJ Smit, NC Guérineau, S Chaumont-D, P Marin The atypical chemokine receptor 3 interacts with Connexin 43 inhibiting astrocytic gap junctional intercellular communication Nat Commun, 2020-09-25;11(1):4855. 2020-09-25 [PMID: 32978390] (Bioassay, Human)
• Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE, 2015-01-28;10(1):e0117908. 2015-01-28 [PMID: 25629162] (Bioassay, Mouse)
• Lacotte S, Decossas M, Le Coz C, Brun S, Muller S, Dumortier H Early differentiated CD138(high) MHCII+ IgG+ plasma cells express CXCR3 and localize into inflamed kidneys of lupus mice. PLoS ONE, 2013-03-08;8(3):e58140. 2013-03-08 [PMID: 23520491] (Bioassay, Mouse)
• Lorena Hernandez, Marco A O Magalhaes, Salvatore J Coniglio, John S Condeelis, Jeffrey E Segall Opposing roles of CXCR4 and CXCR7 in breast cancer metastasis Breast Cancer Research 2011-12-09 [PMID: 22152016] (Neutralization, Rat)
• Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Cxcl11
• Uniprot:
  • Q9JHH5()