Recombinant Mouse CLEC4F/CLECSF13 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse CLEC4F/CLECSF13 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. The ED₅₀ for this effect is 0.75-3.75 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CLEC4F/CLECSF13 protein
  Ala65-Gly548, with an N-terminal 9-His tag
• Accession #: P70194
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: Clec4f
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CLEC4F/CLECSF13 Protein, CF

• CLEC4F
• CLECSF13
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13
• C-type lectin 13
• C-type lectin domain family 4, member F
• Galactose Particle Receptor
• KCLR
• KCR

Background

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1 - 3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N-acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
4. Accession # P70194.
5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

Bioinformatics

• Gene Symbol: Clec4f
• Uniprot:
  • P70194()