Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein Summary
Details of Functionality
Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED50 for this effect is 0.07-0.35 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse CD40 Ligand/TNFSF5 protein
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Theoretical MW
22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
24-29 kDa, reducing conditions
Publications
Read Publications using 8230-CL in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein
CD154 antigen
CD154
CD40 antigen ligand
CD40 Ligand
CD40L
CD40-L
CD40LG
CD40LIGM
gp39
hCD40L
HIGM1
T-B cell-activating molecule
T-BAM
T-cell antigen Gp39
TNF-related activation protein
TNFSF5
TNFSF5IMD3
TRAP
TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
tumor necrosis factor (ligand) superfamily member 5
Tumor necrosis factor ligand superfamily member 5
Background
CD40 Ligand, also known as TNFSF5, CD154, TRAP, or gp39, is a 33-39 kDa type II transmembrane glycoprotein member of the TNF superfamily (1, 2). Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region (1). The extracellular domain of mouse CD40 Ligand shares 75% and 93% amino acid (aa) sequence identity with the human and rat proteins, respectively. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells (3). The 18 kDa soluble form (aa 112-260) arises from proteolytic processing and retains the ability to bind and activate CD40 (4, 5). Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes (2). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (8, 9). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (10, 11).
Armitage, R.J. et al. (1992) Nature 357:80.
Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
Graf, D. et al. (1995) Eur. J. Immunol. 25:1749.
Mazzei, G.J. et al. (1995) J. Biol. Chem. 270:7025.
Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
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FAQs for CD40 Ligand/TNFSF5 (8230-CL). (Showing 1 - 1 of 1 FAQs).
I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.