Recombinant Mouse CCL3/MIP-1 alpha Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse CCL3/MIP-1 alpha Protein Summary

Details of Functionality
Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 5‑25 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 0.4‑2.0 ng/mL.
Source
E. coli-derived mouse CCL3/MIP-1 alpha protein
Ala24-Ala92
Accession #
N-terminal Sequence
Ala24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
450-MA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL3/MIP-1 alpha Protein

  • C-C motif chemokine 3
  • MIP1-(a)
  • AI323804
  • CCL3
  • chemokine (C-C motif) ligand 3
  • G0S19-1
  • LD78a
  • LD78alpha
  • MIP1 alpha
  • MIP-1 alpha
  • Mip1a
  • MIP-1alpha
  • MIP1-alpha
  • Scya3

Background

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature mouse CCL3 shares 73%, 91%, and 82% amino acid sequence identity with human, rat, and cotton rat CCL3, respectively (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8 - 10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11 - 14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (16).
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  2. Davatelis, G. et al. (1988) J. Exp. Med. 167:1939.
  3. Graham, G.J. et al. (1990) Nature 344:442.
  4. Wagner, L. et al. (1998) Nature 391:908.
  5. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
  6. Ren, M. et al. (2010) EMBO J. 29:3952.
  7. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
  8. Taub, D.D. et al. (1993) Science 260:355.
  9. Bernardini, G. et al. (2008) Blood 111:3626.
  10. Lee, S.C. et al. (2000) J. Immunol. 164:3392.
  11. Wu, Y. et al. (2008) J. Immunol. 181:6384.
  12. Cook, D.N. et al. (1995) Science 269:1583.
  13. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
  14. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
  15. Cocchi, F. et al. (1995) Science 270:1811.
  16. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.

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450-MA
Species: Mu
Applications: Bioactivity

Publications for CCL3/MIP-1 alpha (450-MA)(26)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(16)
ELISA (Standard)
(4)
In Vivo
(6)
All Applications
Filter By Species
Human
(1)
Mouse
(20)
Rat
(1)
N/A
(4)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using 450-MA Applications Species
JM da Silva, TP Moreira Do, LM Sobral, CM Queiroz-Ju, MA Rachid, AEI Proudfoot, GP Garlet, AC Batista, MM Teixeira, AM Leopoldino, RC Russo, TA Silva Relevance of CCL3/CCR5 axis in oral carcinogenesis Oncotarget, 2017;8(31):51024-51036. 2017 [PMID: 28881626] (Bioassay, Mouse) Bioassay Mouse
CD84 mediates CLL-microenvironment interactions Oncogene, 2016;0(0):. 2016 [PMID: 27452524] (Bioassay, Mouse) Bioassay Mouse
Couturier J, Stancu I, Schakman O, Pierrot N, Huaux F, Kienlen-Campard P, Dewachter I, Octave J Activation of phagocytic activity in astrocytes by reduced expression of the inflammasome component ASC and its implication in a mouse model of Alzheimer disease. J Neuroinflammation, 2016;13(1):20. 2016 [PMID: 26818951] (Bioassay, Mouse) Bioassay Mouse
Poon G, Dong Y, Marshall K, Arif A, Deeg C, Dosanjh M, Johnson P Hyaluronan Binding Identifies a Functionally Distinct Alveolar Macrophage-like Population in Bone Marrow-Derived Dendritic Cell Cultures. J Immunol, 2015;195(2):632-42. 2015 [PMID: 26085682] (Bioassay, Mouse) Bioassay Mouse
Costa M, de Negreiros C, Bornstein V, Valente R, Mengel J, Henriques M, Benjamim C, Penido C Murine IL-17+ Vgamma4 T lymphocytes accumulate in the lungs and play a protective role during severe sepsis. BMC Immunol, 2015;16(0):36. 2015 [PMID: 26037291] (Bioassay, Mouse) Bioassay Mouse
Mitchell, Duane A, Batich, Kristen, Gunn, Michael, Huang, Min-Nung, Sanchez-Perez, Luis, Nair, Smita K, Congdon, Kendra L, Reap, Elizabet, Archer, Gary E, Desjardins, Annick, Friedman, Allan H, Friedman, Henry S, Herndon, James E, Coan, April, McLendon, Roger E, Reardon, David A, Vredenburgh, James J, Bigner, Darell D, Sampson, John H Tetanus toxoid and CCL3 improve dendritic cell vaccines in mice and glioblastoma patients. Nature, 2015;519(7543):366-9. 2015 [PMID: 25762141] (In Vivo, Mouse) In Vivo Mouse
Sato S, Zhang X The Friend leukaemia virus integration 1 (Fli-1) transcription factor affects lupus nephritis development by regulating inflammatory cell infiltration into the kidney. Clin Exp Immunol, 2014;177(1):102-9. 2014 [PMID: 24580413] (Bioassay, Mouse) Bioassay Mouse
Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S Endothelial cell-derived chemerin promotes dendritic cell transmigration. J Immunol, 2014;192(5):2366-73. 2014 [PMID: 24470498] (Bioassay, Mouse) Bioassay Mouse
Fujihara Y, Takato T, Hoshi K Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration. Stem Cells, 2014;32(5):1208-19. 2014 [PMID: 24446149] (Bioassay, Human) Bioassay Human
Bignon A, Gaudin F, Hemon P, Tharinger H, Mayol K, Walzer T, Loetscher P, Peuchmaur M, Berrebi D, Balabanian K CCR1 inhibition ameliorates the progression of lupus nephritis in NZB/W mice. J Immunol, 2014;192(3):886-96. 2014 [PMID: 24367031] (Bioassay, Mouse) Bioassay Mouse
Show All 26 Publications.

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Bioinformatics

Gene Symbol CCL3
Uniprot