Recombinant Mouse CCL22/MDC Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse CCL22/MDC Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR4. The ED₅₀ for this effect is 0.5-3 ng/mL.
• Source: E. coli-derived mouse CCL22/MDC protein
  Gly25-Ser92
• Accession #: Q9QZU2
• N-terminal Sequence: Gly25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ccl22
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 7.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL22/MDC Protein

• A-152E5.1
• ABCD-1
• CC chemokine STCP-1
• C-C motif chemokine 22
• CCL22
• chemokine (C-C motif) ligand 22
• DC/B-CK
• Macrophage-derived chemokine
• MDC
• MDCStimulated T-cell chemotactic protein 1
• MGC34554
• SCYA22MDC(1-69)
• small inducible cytokine A22
• small inducible cytokine subfamily A (Cys-Cys), member 22
• Small-inducible cytokine A22
• STCP-1
• stimulated T cell chemotactic protein 1

Background

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca²⁺ mobilization in activated mouse and human T cells.

1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Publications for CCL22/MDC (439-MD)(9)

Publications using 439-MD

• W Zhang, X Jiang, Y Zou, L Yuan, X Wang Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma Frontiers in Pharmacology, 2023-03-08;14(0):1092767. 2023-03-08 [PMID: 36969873] (In Vivo, Mouse)
• S Toyama, CS Moniaga, S Nakae, M Kurosawa, H Ogawa, M Tominaga, K Takamori Regulatory T Cells Exhibit Interleukin-33-Dependent Migratory Behavior during Skin Barrier Disruption International Journal of Molecular Sciences, 2021-07-12;22(14):. 2021-07-12 [PMID: 34299063] (Bioassay, Mouse)
• S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-06-25;10(0):1421. 2019-06-25 [PMID: 31293551] (Binding Assay, Mouse)
• ML Ratay, AJ Glowacki, SC Balmert, AP Acharya, J Polat, LP Andrews, MV Fedorchak, JS Schuman, DAA Vignali, SR Little Treg-recruiting microspheres prevent inflammation in a murine model of dry eye disease J Control Release, 2017-05-10;0(0):. 2017-05-10 [PMID: 28501670] (Bioassay, In Vivo, Mouse)
• Beverley P, Ruzsics Z, Hey A, Hutchings C, Boos S, Bolinger B, Marchi E, O'Hara G, Klenerman P, Koszinowski U, Tchilian E A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis. J Immunol, 2014-07-28;193(5):2306-16. 2014-07-28 [PMID: 25070842] (In Vivo, Mouse)
• Loebbermann , Jens, Durant , Lydia, Thornton , Hannah, Johansson , Cecilia, Openshaw , Peter J Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells. Proc Natl Acad Sci U S A, 2013-02-04;110(8):2987-92. 2013-02-04 [PMID: 23382205] (In Vivo, Mouse)
• Kearley J, Buckland KF, Mathie SA, Lloyd CM Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81. 2009-01-29 [PMID: 19179489] (ELISA (Standard))
• Kearley J, Robinson DS, Lloyd CM CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling. J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6. 2008-07-30 [PMID: 18672278] (ELISA (Standard))
• Fujita H, Asahina A, Komine M, Tamaki K The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell. Immunol., 2007-05-15;245(2):70-9. 2007-05-15 [PMID: 17507001] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ccl22
• Uniprot:
  • Q9QZU2()