Recombinant Mouse CCL1/TCA-3 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse CCL1/TCA-3 Protein Summary

Details of Functionality
Measured by its ability to chemoattract BW5147 mouse T lymphoma cells. The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR8. The ED50 for this effect is 0.5-2.0 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CCL1/I-309/TCA-3 protein
Lys24-Cys92
Accession #
N-terminal Sequence
Lys24
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13-16 kDa, reducing conditions
Publications
Read Publications using
845-TC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL1/TCA-3 Protein

  • C-C motif chemokine 1
  • CCL1
  • chemokine (C-C motif) ligand 1
  • I-309
  • inflammatory cytokine I-309
  • P500
  • SCYA1
  • SCYA1Small-inducible cytokine A1
  • SCYA2
  • SISe
  • small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
  • T lymphocyte-secreted protein I-309
  • TCA3
  • TCA-3

Background

Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.

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Publications for CCL1/I-309/TCA-3 (845-TC)(7)

We have publications tested in 4 confirmed species: Mouse, Mouseq, N/A, Virus.

We have publications tested in 2 applications: Bioassay, ELISA Standard.


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Bioassay
(5)
ELISA Standard
(2)
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Mouse
(3)
Mouseq
(1)
N/A
(2)
Virus
(1)
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Showing Publications 1 - 7 of 7.
Publications using 845-TC Applications Species
Y Xu, X Dong, P Qi, Y Ye, W Shen, L Leng, L Wang, X Li, X Luo, Y Chen, P Sun, R Xiang, N Li Sox2 Communicates With Tregs Through CCL1 To Promote The Stemness Property Of Breast Cancer Cells Stem Cells, 2017;0(0):. 2017 [PMID: 29044882] (Bioassay, Mouseq) Bioassay Mouseq
SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin J. Immunol, 2016;196(6):2514-25. 2016 [PMID: 26851219] (Bioassay, Mouse) Bioassay Mouse
Wu Z, MacNeil A, Junkins R, Li B, Berman J, Lin T Mast cell FcepsilonRI-induced early growth response 2 regulates CC chemokine ligand 1-dependent CD4+ T cell migration. J Immunol, 2013;190(9):4500-7. 2013 [PMID: 23536637] (Bioassay, Mouse) Bioassay Mouse
Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011;12(2):167-77. 2011 [PMID: 21217759] (Bioassay, Mouse) Bioassay Mouse
Kearley J, Buckland KF, Mathie SA, Lloyd CM Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Am. J. Respir. Crit. Care Med., 2009;179(9):772-81. 2009 [PMID: 19179489] (ELISA Standard, N/A) ELISA Standard N/A
Kearley J, Robinson DS, Lloyd CM CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling. J. Allergy Clin. Immunol., 2008;122(3):617-24.e6. 2008 [PMID: 18672278] (ELISA Standard, N/A) ELISA Standard N/A
Najarro P, Lee HJ, Fox J, Pease J, Smith GL Yaba-like disease virus protein 7L is a cell-surface receptor for chemokine CCL1. J. Gen. Virol., 2003;84(0):3325-36. 2003 [PMID: 14645913] (Bioassay, Virus) Bioassay Virus

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Bioinformatics

Gene Symbol Ccl1
Entrez
Uniprot