Recombinant Mouse Angiopoietin-3 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Angiopoietin-3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can bind rmAngiopoietin-3 with a linear range of 5-100 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Angiopoietin-3 protein
  

  Mouse Angiopoietin-3 (Gln22 - Ala509) Accession # Q9WVH6	IEGR	GGGSGGGSGGGS	10-His tag
  N-terminus			C-terminus

• Accession #: Q9WVH6
• N-terminal Sequence: No results obtained: Gln22 predicted
• Structure / Form: Oligomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Angpt4
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Angiopoietin-3 Protein, CF

• ANG3
• ANG4
• Angiopoietin-3
• ANGPT3

Background

Mouse angiopoietin-3 (ANG-3) (1), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Mouse ANG-3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. It shares 47%, 46% and 54% aa sequence identity with mouse ANG-1, mouse ANG-2 and human ANG-4, respectively. Although the sequence homology is much higher between the human and mouse counterparts for ANG-1 (97%) and ANG-2 (85%), mouse ANG-3 is believed to be an ortholog of human ANG-4 based on chromosomal localization studies (1, 2). Human ANG-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse ANG-3 is expressed in multiple mouse tissues. Human ANG-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse ANG-3 has been reported to be a Tie-2 antagonist. It is likely that mouse ANG-3, like ANG-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Publications for Angiopoietin-3 (738-AN/CF)(1)

Publication using 738-AN/CF

• H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina Elife, 2018-11-16;7(0):. 2018-11-16 [PMID: 30444491] (Bioassay, ELISA (Capture), Surface Plasmon Resonance, Human)

Bioinformatics

• Gene Symbol: Angpt4
• Uniprot:
  • Q9WVH6()