Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmActivin RIB/Fc Chimera at 1 µg/mL (100 µL/well) can bind rmCripto with a linear range of 0.8‑100 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Activin RIB/ALK-4 protein
  

  Mouse Activin RIB (Leu32 - Glu126) Accession #NP_031421	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: NP_031421
• N-terminal Sequence: Leu32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Acvr1b
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 37.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 44 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Activin RIB/ALK-4 Fc Chimera Protein, CF

• activin A receptor, type IB
• Activin receptor type IB
• Activin receptor-like kinase 4
• Activin RIB
• ActivinRIB
• ActRIB
• ACVR1B
• ACVRLK4ACTR-IB
• ALK4
• ALK-4
• ALK4activin A receptor, type II-like kinase 4
• EC 2.7.11
• EC 2.7.11.30
• Serine/threonine-protein kinase receptor R2
• SKR2activin receptor type-1B

Background

Activin RIB, also known as ALK-4, is a glycosylated 58 kDa type I transmembrane receptor that belongs to the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with, and phosphorylates, the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature mouse Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, mouse Activin RIB shares 93% and 98% aa sequence identity with human and rat Activin RIB, respectively. It shares 23% - 38% aa sequence identity with other mouse type I receptors Activin RIA, BMPR-IA, BMPR-IB, and TGF-beta RI. Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (5 - 7). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (8). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation, apoptosis, and tumorigenesis (1, 9, 10).

1. Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
2. Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
3. Tsuchida, K. et al. (1995) Endocrinology 136:5493.
4. Ebner, R. et al. (1993) Science 260:1344.
5. Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
6. Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
8. Lewis, K.A. et al. (2000) Nature 404:411.
9. Gu, Z. et al. (1998) Genes Dev. 12:844.
10. Chen, Y. et al. (2004) Dev. Biol. 268:280.

Publications for Activin RIB/ALK-4 (1477-AR)(1)

Publication using 1477-AR

• Niessen K, Zhang G, Ridgway JB, Chen H, Yan M ALK1 signaling regulates early postnatal lymphatic vessel development. Blood, 2009-11-10;115(8):1654-61. 2009-11-10 [PMID: 19903896] (ELISA (Standard))

Bioinformatics

• Gene Symbol: Acvr1b
• Uniprot:
  • NP_031421()