Recombinant Mouse 2B4/CD244/SLAMF4 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse 2B4/CD244/SLAMF4 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rm2B4/Fc Chimera at 5 µg/mL (100 µL/well) can bind rmCD48 with a linear range of 0.15-10 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse 2B4/CD244/SLAMF4 protein
  

  Mouse 2B4 (His17-Asn221) Accession #Q07763	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q07763
• N-terminal Sequence: His17
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd244
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 50 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse 2B4/CD244/SLAMF4 Fc Chimera Protein, CF

• 2B4
• CD244 antigen
• CD244 molecule, natural killer cell receptor 2B4
• CD244
• h2B4
• NAIL
• NAILNK cell activation-inducing ligand
• natural killer cell receptor 2B4
• NKR2B4
• NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4
• Nmrk
• SLAMF4
• SLAMF4NK cell activation inducing ligand NAIL

Background

2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig‑like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact in a homophilic manner (1‑4). The mouse 2B4 cDNA encodes a 397 amino acid (aa) precursor that includes a 19 aa signal sequence, a 207 aa ECD with one Ig-like V-type and one C2-type Ig-like domain, a 21 aa transmembrane segment, and a 150 aa cytoplasmic domain with four ITSMs (5, 6). A short splice variant of mouse 2B4 contains only one ITSM (6). Within the ECD, mouse 2B4 shares 46% and 68% aa sequence identity with human and rat 2B4, respectively. The ECD of mouse 2B4 shares 17‑26% aa sequence identity with comparable regions of mouse BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma δ T cells, monocytes, some CD4⁺ and CD8⁺ T cells, and dendritic cells (7). CD48 mediates 2B4⁺ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8‑10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8⁺ T cell activation (7‑13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). 2B4 can also induce signaling through CD48 (10, 14). The inhibitory effect is associated with the long form of 2B4, while activation is associated with the short form (9, 15).

1. Bhat, R. et al. (2006) J. Leukocyte Biol. 79:417.
2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
3. McNerney, M.E. et al. (2005) Mol. Immunol. 42:489.
4. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
5. Mathew, P.A. et al. (1993) J. Immunol. 151:5328.
6. Stepp, S.E. et al. (1999) Eur. J. Immunol. 29:2392.
7. Nakajima, H. et al. (1999) Eur. J. Immunol. 29:1676.
8. Lee, K.M. et al. (2006) Blood 107:3181.
9. Mooney, J.M. et al. (2004) J. Immunol. 173:3953.
10. Assarsson, E. et al. (2004) J. Immunol. 173:174.
11. Bryceson, Y.T. et al. (2006) Blood 107:159.
12. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
13. McNerney, M.E. et al. (2005) Blood 106:1337.
14. Messmer, B. et al. (2006) J. Immunol. 176:4646.
15. Schatzle, J.D. et al. (1999) Proc. Natl. Acad. Sci. 96:3870.

Bioinformatics

• Gene Symbol: Cd244
• Uniprot:
  • Q07763()