Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF

Recombinant Human Wnt-1/sFRP-1 Complex (Catalog # 11160-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 25.0-250 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF Summary

• Details of Functionality: Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 25.0-250 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Wnt-1 protein
  

  Human Wnt-1 (Ala28-Leu370) Accession # P04628.1

  Human sFRP-1 (Ser32-Lys314) Accession # AAB70793.1
  N-terminus	C-terminus

• N-terminal Sequence: Ala28 (Wnt-1) & Ser32 (sFRP-1)
• Structure / Form: Non-covalent complex
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 38 kDa (Wnt-1) & 33 kDa (sFRP-1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-58 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF

• Int-1
• INT1Proto-oncogene Int-1 homolog
• proto-oncogene Wnt-1
• wingless-type MMTV integration site family, member 1 (oncogene INT1)
• wingless-type MMTV integration site family, member 1
• Wnt1
• Wnt-1

Background

Wnt-1 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Human Wnt-1 cDNA encodes a 370 amino acid (aa) protein that contains four potential N-linked glycosylation sites plus 23 cysteines (2, 3). When secreted, it exists as a 343 aa glycoprotein with a molecular weight of 42 to 48 kDa. Human Wnt -1 shares a 99.7% aa sequence identity with mouse Wnt-1. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (4). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (5). Wnt-1 is known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). R&D Systems produces biologically active human Wnt-1 protein in a complex with human sFRP-1, and this Wnt-1/sFRP-1 complex is stable in the physiological buffer without detergent.

1. Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
2. Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
3. Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
4. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
5. Korinek, V. et al. (1997) Science 275:1784.
6. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
7. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.