Recombinant Human TROP-2 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TROP-2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells or LS180 human colorectal adenocarcinoma cells. When 1 x 10⁴ to 5 x 10⁴ cells/well are added to Recombinant Human TROP‑2 Fc Chimera coated plates (10 µg/mL with 100 µL/well), >45% cells will adhere after 1 hour incubation.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human TROP-2 protein
  

  Human TROP-2 (Thr88-Thr274) Accession # P09758.3	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: P09758.3
• N-terminal Sequence: Thr88
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: TACSTD2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TROP-2 Fc Chimera Protein, CF

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Publications for TROP-2 (650-T2)(4)

Publications using 650-T2

• Bangayan, NJ;Wang, L;Burton Sojo, G;Noguchi, M;Cheng, D;Ta, L;Gunn, D;Mao, Z;Liu, S;Yin, Q;Riedinger, M;Li, K;Wu, AM;Stoyanova, T;Witte, ON; Dual-inhibitory domain iCARs improve the efficiency of the AND-NOT gate CAR T strategy Proceedings of the National Academy of Sciences of the United States of America 2023-11-21 [PMID: 37963244] (Bioassay, Human)
• Guerra, E;Trerotola, M;Relli, V;Lattanzio, R;Ceci, M;Boujnah, K;Pantalone, L;Di Pietro, R;Iezzi, M;Tinari, N;Alberti, S; The 2EF Antibody Targets a Unique N-Terminal Epitope of Trop-2 and Enhances the In Vivo Activity of the Cancer-Selective 2G10 Antibody Cancers 2023-07-22 [PMID: 37509383] (ELISA Capture, Human)
• H Uemachi, Y Kasahara, K Tanaka, T Okuda, Y Yoneda, S Obika Hybrid-Type SELEX for the Selection of Artificial Nucleic Acid Aptamers Exhibiting Cell Internalization Activity Pharmaceutics, 2021-06-15;13(6):. 2021-06-15 [PMID: 34204006] (Bioassay, Human)
• Leitner , Judith, Rieger , Armin, Pickl , Winfried, Zlabinger , Gerhard, Grabmeier-Pfistershammer , Katharin, Steinberger , Peter TIM-3 does not act as a receptor for galectin-9. PLoS Pathog, 2013-03-21;9(3):e1003253. 2013-03-21 [PMID: 23555261] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • P09758.3()