Recombinant Human TrkC Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TrkC Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit NT-3-induced proliferation of BaF-TrkB-BD mouse pro-B cells transfected with TrkB. The ED 50 for this effect is 0.05-0.2 µg/mL in the presence of 40 ng/mL of rhNT-3.
• Source: Mouse myeloma cell line, NS0-derived human TrkC protein

  Human TrkC (Cys32 - Asp428) Accession # Q96CY4

  DIEGRMD

  Human IgG 1 (Pro100 - Lys330)

  6-His tag

  N-terminus

  C-terminus

• Accession #: Q96CY4
• N-terminal Sequence: Cys32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: NTRK3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 72 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TrkC Fc Chimera Protein, CF

• EC 2.7.10
• EC 2.7.10.1
• ETS related protein-neurotrophic receptor tyrosine kinase fusion protein
• ETV6-NTRK3 fusion
• gp145(trkC)
• GP145-TrkC
• Neurotrophic tyrosine kinase receptor type 3
• neurotrophic tyrosine kinase, receptor, type 3
• NT 3 receptor
• NT-3 growth factor receptor
• NTRK3
• TrkC tyrosine kinase
• TrkC
• trk-C
• TRKCneurotrophin 3 receptor
• tyrosine kinase receptor C

Background

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1 - 2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC's showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

1. Huang, E.J. and L.F. Reichardt. (2003) Annu. Rev. Biochem. 72:(epub ahead of print).
2. Dechant, G. (2001) Cell Tissue Res. 305:229.
3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
5. Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
6. Shelton, D. et al. (1995) J. Neurosci. 15:477.

Publications for TrkC (373-TC/CF)(6)

Publications using 373-TC/CF

• Y Guo, ZJ Su, YK Chen, Z Chai BDNF/NT3 Regulate AIS Location and Affect Neuronal Excitability in Cultured Hippocampal Neurons J. Neurochem., 2017-05-26;0(0):. 2017-05-26 [PMID: 28440877] (Bioassay, Rat)
• JN Sleigh, JM Dawes, SJ West, N Wei, EL Spaulding, A Gómez-Mart, Q Zhang, RW Burgess, MZ Cader, K Talbot, XL Yang, DL Bennett, G Schiavo Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations Proc. Natl. Acad. Sci. U.S.A, 2017-03-28;0(0):. 2017-03-28 [PMID: 28351971] (Bioassay)
• Vaegter CB, Jansen P, Fjorback AW Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling. Nat. Neurosci., 2010-11-21;14(1):54-61. 2010-11-21 [PMID: 21102451] (Surface Plasmon Resonance)
• Bouzas-Rodriguez J, Cabrera JR, Delloye-Bourgeois C, Ichim G, Delcros JG, Raquin MA, Rousseau R, Combaret V, Benard J, Tauszig-Delamasure S, Mehlen P Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis. J. Clin. Invest., 2010-02-15;120(3):850-8. 2010-02-15 [PMID: 20160348] (Bioassay, Human)
• Lu B, PereiraPerrin M A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008-06-09;76(8):3530-8. 2008-06-09 [PMID: 18541656] (PcIP, Bacteria)
• Esper RM, Loeb JA Rapid axoglial signaling mediated by neuregulin and neurotrophic factors. J. Neurosci., 2004-07-07;24(27):6218-27. 2004-07-07 [PMID: 15240814] (Bioassay, Chicken)

Bioinformatics

• Gene Symbol: NTRK3
• Uniprot:
  • Q96CY4()