Recombinant Human TRANCE/RANK L/TNFSF11 (E. coli-expressed)


Recombinant Human TRANCE/RANK L/TNFSF11 (Catalog # 6449-TEC) has a molecular weight (MW) of 55.4 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to more
Recombinant Human TRANCE/RANK L/TNFSF11 (6449-TEC) induces osteoclast differentiation of the RAW264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 2-12 ng/mL.

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Applications Bioactivity

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Recombinant Human TRANCE/RANK L/TNFSF11 (E. coli-expressed) Summary

Details of Functionality
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2-12 ng/mL
E. coli-derived human TRANCE/TNFSF11/RANK L protein
Arg85-Asp245, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
19 kDa, reducing conditions
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6449-TEC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MES and NaCl with BSA as a carrier protein.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TRANCE/RANK L/TNFSF11 (E. coli-expressed)

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11


RANK L (receptor activator of NF-kappa B ligand), also called TRANCE (TNF-related activation-induced cytokines), OPGL (osteoprotegerin ligand), or ODF (osteoclast differentiation factor), is a 39‑45 kDa type II transmembrane (TM) protein in the tumor necrosis factor family, designated TNFSF11 (1‑5). RANK L, produced by osteoblasts and bone marrow stromal cells, is required for differentiation of osteoclasts and stimulates bone resorption (4, 6). It is also produced by activated T cells and augments dendritic cell stimulation; RANK L-/- mice lack lymph nodes and have impaired thymocyte development (1‑3, 6). The human RANK L cDNA encodes 317 amino acids (aa), including a 47 aa cytoplasmic domain, a 21 aa TM region, and a 249 aa extracellular domain (ECD) with two potential N‑linked glycosylation sites (note: Arg85‑Asp245 of Accession # AAC51762 is identical to Arg157‑Asp317 of SwissProt # O14788. This aa range contains the ECD trimerization and receptor‑binding motifs, but not ECD proteolytic cleavage sites). Within the ECD, human RANK L shares 89%, 89%, 93% and 95% aa identity with mouse, rat, bovine and porcine RANK L, respectively. Mouse RANK L can stimulate human osteoclast differentiation (4). Like most TNF family members, RANK L can form trimers (1). Soluble 31, 25 and 24 kDa forms of RANK L can be created by usage of alternate start sites at aa 74 or 146, or proteolytic cleavage by osteoblast- or stromal cell‑derived ADAM10 (after aa 139) or MMP14 (aa 146), or bone metastatic prostate tumor-derived MT1-MMP (aa 146) (5, 7, 8). Both TM and soluble extracellular RANK L act by engaging RANK receptors and are antagonized by the decoy receptor, OPG (osteoprotegrin) (2, 5). In resting cells, the majority of RANK L is stored in secretory lysosomes (9). In mammary epithelia, RANK L is upregulated by pregnancy hormones and is essential for the formation of a lactating mammary gland (10). In the brain, astrocyte RANK L mediates body temperature regulation (11). Pathologically, RANK L is thought to mediate post-menopausal osteoporosis, vascular calcification, progestin-induced breast cancer, cancer-induced bone disease, and osteopetrosis (in RANK L deficiencies) (12‑16).

  1. Leibbrandt, A. and  J.M. Penninger 2008) Ann. N. Y. Acad. Sci. 1143:123.
  2. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  3. Anderson, D.M. et al. (1997) Nature 390:175.
  4. Lacey, D.L. et al. (1998) Cell 93:165.
  5. Hikita, A. et al. (2006) J. Biol. Chem. 281:36846.
  6. Kong, Y-Y. et al. (1999) Nature 397:315.
  7. Accession #NP_143026 and EAX08679.
  8. Sabbota, A.L. et al. (2010) Cancer Res. 70:5558.
  9. Aoki, S. et al. (2010) J. Bone Miner. Res. 25:1907.
  10. Fata, J.E. et al. (2000) Cell 103:41.
  11. Hanada, R. et al. (2009) Nature 426:505.    
  12. Osako, M.K. et al. (2010) Circ. Res. 107:466.
  13. Schramek, D. et al. (2010) Nature 468:98.
  14. Gonzalez-Suarez, E. et al. (2010) Nature 468:103.
  15. Dougall, W.C. and M. Chaisson (2006) Cancer Metastasis Rev. 25:541.
  16. Sobacchi, C. et al. (2007) Nat. Genet. 39:960.

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The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Gene Symbol TNFSF11