Recombinant Human Tie-2 Fc Chimera Protein, CF

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When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin‑2 (Catalog # 623-AN) binds with an ED50 of 0.5‑4 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Tie-2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin‑2 (Catalog # 623-AN) binds with an ED50 of 0.5‑4 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Tie-2 protein
Human Tie-2
(Ala23-Lys745)
Accession # Q02763
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TEK
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
100 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
135 kDa, reducing conditions
Publications
Read Publications using
313-TI in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Tie-2 Fc Chimera Protein, CF

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
  3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.

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Publications for Tie-2 (313-TI)(13)

We have publications tested in 5 confirmed species: Human, Mouse, N/A, Porcine, Yeast - Saccharomyces cerevisiae.

We have publications tested in 5 applications: Binding Assay, Bioassay, ELISA, ELISA (Standard), Functional ELISA.


Filter By Application
Binding Assay
(1)
Bioassay
(7)
ELISA
(1)
ELISA (Standard)
(2)
Functional ELISA
(2)
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Filter By Species
Human
(8)
Mouse
(1)
N/A
(2)
Porcine
(1)
Yeast - Saccharomyces cerevisiae
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 313-TI Applications Species
H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina Elife, 2018;7(0):. 2018 [PMID: 30444491] (ELISA, Human) ELISA Human
T Shlamkovic, L Aharon, WA Barton, N Papo Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy Oncotarget, 2017;8(20):33571-33585. 2017 [PMID: 28422724] (Bioassay, Yeast - Saccharomyces cerevisiae) Bioassay Yeast - Saccharomyces cerevisiae
Wilson Chan Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells PLoS ONE, 2016;11(6):e0156994. 2016 [PMID: 27304216] (ELISA (Standard), Human) ELISA (Standard) Human
Yao J, Cui M, Li M, Liu Y, He Q, Xiao J, Bai Y Angiopoietin1 inhibits mast cell activation and protects against anaphylaxis. PLoS ONE, 2014;9(2):e89148. 2014 [PMID: 24586553] (Bioassay, Mouse) Bioassay Mouse
Buchanan A, Clementel V, Woods R, Harn N, Bowen M, Mo W, Popovic B, Bishop S, Dall'Acqua W, Minter R, Jermutus L, Bedian V Engineering a therapeutic IgG molecule to address cysteinylation, aggregation and enhance thermal stability and expression. MAbs, 2013;5(2):255-62. 2013 [PMID: 23412563] (Functional ELISA, Human) Functional ELISA Human
Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Nat. Cell Biol., 2008;10(5):527-37. 2008 [PMID: 18425119] (Bioassay, Human) Bioassay Human
Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2. Mol. Biol. Cell, 2006;17(12):5163-72. 2006 [PMID: 17035633] (Bioassay, Human) Bioassay Human
Red-Horse K, Rivera J, Schanz A, Zhou Y, Winn V, Kapidzic M, Maltepe E, Okazaki K, Kochman R, Vo KC, Giudice L, Erlebacher A, McCune JM, Stoddart CA, Fisher SJ Cytotrophoblast induction of arterial apoptosis and lymphangiogenesis in an in vivo model of human placentation. J. Clin. Invest., 2006;116(10):2643-52. 2006 [PMID: 16998586] (Bioassay, Human) Bioassay Human
Zhu T, Sennlaub F, Beauchamp MH, Fan L, Joyal JS, Checchin D, Nim S, Lachapelle P, Sirinyan M, Hou X, Bossolasco M, Rivard GE, Heveker N, Chemtob S Proangiogenic effects of protease-activated receptor 2 are tumor necrosis factor-alpha and consecutively Tie2 dependent. Arterioscler. Thromb. Vasc. Biol., 2006;26(4):744-50. 2006 [PMID: 16439712] (Bioassay, Porcine) Bioassay Porcine
Popkov M, Jendreyko N, McGavern DB, Rader C, Barbas CF Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody. Cancer Res., 2005;65(3):972-81. 2005 [PMID: 15705898] (Binding Assay, N/A) Binding Assay N/A
Show All 13 Publications.

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Bioinformatics

Gene Symbol TEK
Uniprot