Recombinant Human Thrombopoietin Protein, CF

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1 µg/lane of Recombinant Human Thrombopoietin was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 43-60 kDa.
Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP/CF) stimulates pro­liferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.3-3 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant Human Thrombopoietin Protein, CF Summary

Additional Information
A New rhTpo is Available! NS0 expressed; Lower endotoxin spec; Competitively priced!
New Product 288-TPN
Details of Functionality
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.3-3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Thrombopoietin/Tpo protein
Ser22-Gly353
Accession #
N-terminal Sequence
Ser22
Protein/Peptide Type
Recombinant Proteins
Gene
THPO
Purity
>97%, by SDS-PAGE with silver staining
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
43-60 kDa, reducing conditions
Publications
Read Publications using
288-TP/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 50-200 µg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Thrombopoietin Protein, CF

  • Megakaryocyte colony-stimulating factor
  • Megakaryocyte growth and development factor
  • megakaryocyte stimulating factor
  • MGDF
  • MGDFC-mpl ligand
  • MK-CSF
  • ML
  • MPL ligand
  • MPLLGMGC163194
  • Myeloproliferative leukemia virus oncogene ligand
  • THPO
  • thrombopoietin nirs variant 1
  • Thrombopoietin
  • Tpo
  • TPOMKCSF

Background

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c‑mpl. Defects in the Tpo‑Tpo R signaling pathway are associated with a variety of platelet disorders (1‑3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80‑85 kDa protein that consists of an N‑terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N‑linked and O‑linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL‑3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10, 13). It is cleaved by platelet‑derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia‑sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).

  1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
  2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
  3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
  4. Bartley, T.D. et al. (1994) Cell 77:1117.
  5. de Sauvage, F.J. et al. (1994) Nature 369:533.
  6. Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
  7. Kaushansky, K. et al. (1994) Nature 369:568.
  8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
  9. Broudy, V.C. et al. (1995), Blood 85:1719.
  10. Lok, S.I. et al. (1994) Nature 369:565.
  11. Chen, J. et al. (1995) Blood 86:4054.
  12. Oda, A. et al. (1996) Blood 87:4664.
  13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
  14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
  15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
  16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.

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288-TP/CF
Species: Hu
Applications: Bioactivity

Publications for Thrombopoietin/Tpo (288-TP/CF)(50)

We have publications tested in 8 confirmed species: Human, Mouse, Canine, N/A, Primate - Baboon, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 4 applications: Bioassay, ELISA Ctrl, WB Ctrl, cell culture.


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Bioassay
(45)
ELISA Ctrl
(1)
WB Ctrl
(1)
cell culture
(2)
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Human
(36)
Mouse
(3)
Canine
(1)
N/A
(2)
Primate - Baboon
(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(4)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using 288-TP/CF Applications Species
M Cesana, MH Guo, D Cacchiarel, L Wahlster, J Barragan, S Doulatov, LT Vo, B Salvatori, C Trapnell, K Clement, P Cahan, KM Tsanov, PM Sousa, B Tazon-Vega, A Bolondi, FM Giorgi, A Califano, JL Rinn, A Meissner, JN Hirschhorn, GQ Daley A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development Cell Stem Cell, 2018;22(4):575-588.e7. 2018 [PMID: 29625070] (Bioassay, Human) Bioassay Human
A Palau, AK Garz, J Diesch, A Zwick, R Malinverni, V Valero, K Lappin, R Casquero, A Lennartsso, J Zuber, T Navarro, KI Mills, KS Götze, M Buschbeck Polycomb protein RING1A limits hematopoietic differentiation in myelodysplastic syndromes Oncotarget, 2017;8(70):115002-115017. 2017 [PMID: 29383137] (Bioassay, Human) Bioassay Human
NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis Cell Rep, 2017;20(7):1597-1608. 2017 [PMID: 28813672] (Bioassay, Human) Bioassay Human
JH Lee, KR Salci, JC Reid, L Orlando, B Tanasijevi, Z Shapovalov, M Bhatia Brief Report: Human Acute Myeloid Leukemia Reprograming to Pluripotency Is a Rare Event and Selects for Patient Hematopoietic Cells Devoid of Leukemic Mutations Stem Cells, 2017;0(0):. 2017 [PMID: 28758276] (Bioassay, Human) Bioassay Human
J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease Mol Ther Methods Clin Dev, 2017;5(0):241-258. 2017 [PMID: 28603745] (Bioassay, Human) Bioassay Human
R Sugimura, DK Jha, A Han, C Soria-Vall, EL da Rocha, YF Lu, JA Goettel, E Serrao, RG Rowe, M Malleshaia, I Wong, P Sousa, TN Zhu, A Ditadi, G Keller, AN Engelman, SB Snapper, S Doulatov, GQ Daley Haematopoietic stem and progenitor cells from human pluripotent stem cells Nature, 2017;545(7655):432-438. 2017 [PMID: 28514439] (Bioassay, Human) Bioassay Human
KL Shaw, E Garabedian, S Mishra, P Barman, A Davila, D Carbonaro, S Shupien, C Silvin, S Geiger, B Nowicki, EM Smogorzews, B Brown, X Wang, S de Oliveir, Y Choi, A Ikeda, D Terrazas, PY Fu, A Yu, BC Fernandez, AR Cooper, B Engel, G Podsakoff, A Balamuruga, S Anderson, L Muul, GJ Jagadeesh, N Kapoor, J Tse, TB Moore, K Purdy, R Rishi, K Mohan, S Skoda-Smit, D Buchbinder, RS Abraham, A Scharenber, OO Yang, K Cornetta, D Gjertson, M Hershfield, R Sokolic, F Candotti, DB Kohn Clinical efficacy of gene-modified stem cells in adenosine deaminase-deficient immunodeficiency J. Clin. Invest, 2017;0(0):. 2017 [PMID: 28346229] (Bioassay, Human) Bioassay Human
B Guo, X Huang, S Cooper, HE Broxmeyer Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment Nat. Med, 2017;0(0):. 2017 [PMID: 28263313] (Bioassay, Human) Bioassay Human
AG Kotini, CJ Chang, A Chow, H Yuan, TC Ho, T Wang, S Vora, A Solovyov, C Husser, M Olszewska, J Teruya-Fel, D Perumal, VM Klimek, A Spyridonid, RK Rampal, L Silverman, EP Reddy, E Papaemmanu, S Parekh, BD Greenbaum, CS Leslie, MG Kharas, EP Papapetrou Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28215825] (Bioassay, Human) Bioassay Human
I Baccelli, J Krosl, G Boucher, I Boivin, VP Lavallée, J Hébert, S Lemieux, A Marinier, G Sauvageau A novel approach for the identification of efficient combination therapies in primary human acute myeloid leukemia specimens Blood Cancer J, 2017;7(2):e529. 2017 [PMID: 28211886] (Bioassay, Human) Bioassay Human
Show All 50 Publications.

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Bioinformatics

Gene Symbol THPO
Entrez
Uniprot